Gut microbial metabolites in lung cancer development and immunotherapy: Novel insights into gut-lung axis

Cancer Letters, Journal Year: 2024, Volume and Issue: 598, P. 217096 - 217096

Published: July 4, 2024

Language: Английский

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An Advanced Multivalent Ligand‐Decorated Microsphere Enrichment System Efficiently Captures Circulating Tumor Cells In Vivo

Small, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

Abstract Capturing circulating tumor cells (CTCs) in vivo from the bloodstream lessens metastasis and recurrence risks. However, absence of CTC receptors due to epithelial‐mesenchymal transition (EMT), limited binding capacity a single ligand, complexity blood flow environment significantly reduce efficiency capture vivo. Herein, multivalent ligand‐decorated microsphere enrichment system (MLMES) is crafted that incorporates column replete with an immunosorbent precisely recognizes binds stably expressed cluster differentiation 44 (CD44) glucose transporter protein 1 (GLUT1) present on exterior CTCs. As peripheral flows through column, CTCs are efficiently captured, achieving rate up 64.2%, highest reported date. Moreover, MLMES demonstrates excellent biocompatibility, broad‐spectrum capture, storage stability. Importantly, it eliminates substantial quantity blood, reducing risk metastasis. This breakthrough method has broad clinical application potential preventing recurrence, bringing new possibilities for improving cancer treatment.

Language: Английский

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Ultrasensitive Electrochemical Screening of Circulating Tumor Cells Over Single‐Atom Nanozyme Integrated 3D Nanoarray

Small, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 9, 2025

Late-stage diagnosis is a major contributor to cancer mortality and thus leads increased fatality, making early detection crucial for improving survival rates. Circulating tumor cells (CTC), detectable before primary tumors become clinically apparent, have emerged as vital biomarkers the identification of aggressive cancers. Here, develop single-atom nanozyme integrated nanoarray 3D nano-biointerface ultrasensitive electrochemical screening CTCs from hepatocellular carcinoma. This cytosensor capable identifying CTC at single-cell level, achieving an impressive area under curve 0.96 in receiver operating characteristics, comparable simulated multi-indicator diagnostic strategies. strategy shows great potential non-invasive carcinoma promising be applied universally diagnosis.

Language: Английский

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Filter‐Assisted ICP‐MS Tumor Liquid Biopsy Enabled by Dual‐Target‐Regulated Functional DNA Nanospheres Cascade Amplification

Small Methods, Journal Year: 2025, Volume and Issue: unknown

Published: March 22, 2025

Abstract An ultrasensitive ICP‐MS aptasensor is developed utilizing a label‐free, simple filter membrane‐assisted separation technique combined with nucleic acid signal amplification for the analysis of circulating tumor cells (CTCs) in lung cancer clinical samples. The approach based on high‐affinity interaction between aptamers and PD‐L1 mucin 1, which are overexpressed cell surface, conjunction functional Y‐DNA nanospheres catalytic hairpin assembly amplifications, enabling simultaneous detection two proteins. Additionally, four‐armed nanostructure significant spatial site resistance self‐assembled by introducing streptavidin biotinylated‐hairpin structures, improving efficiency membrane. This structural design enables effective isolation biotin‐T‐Hg 2+ ‐T biotin‐C‐Ag + ‐C from free Hg Ag , facilitating highly sensitive dual‐protein via ICP‐MS. limits reached ag mL −1 levels proteins single‐cell A549 cells. CTCs extracted whole blood samples patients within 45 min through centrifugation procedure. Quantification performed 37 samples, demonstrating results consistent diagnoses. assay exhibits specificity 100% sensitivity 94.5%.

Language: Английский

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One Step Visual Homogeneous Immunoassay of a Rheumatoid Arthritis Biomarker in Serum via Target-Regulated Steric Hindrance and Competitive Recognition

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 26, 2025

Homogeneous analysis techniques offer several advantages as alternatives to heterogeneous immunoassays, such simplicity and rapidity. In this study, a visual homogeneous immunoassay without labeling process was developed based on target-induced steric hindrance regulate competitive recognition mechanism. Specifically, the analyte concentration varies, change of microenvironment could affect Cu2+ by signal probes. Herein, taking anticyclic citrullinated peptide antibody (anti-CCP) an example, method verified. can bind histidine (His), well Cyclic containing His served capture antigen, fluorescence both CdTe quantum dots calcein be quenched Cu2+. Then, quenching effect regulated hindrance, so that anti-CCP realized. The limit detection low 0.002 0.01 U/mL in mode red, green, blue (RGB) mode, respectively. Furthermore, clinical practicality validated through 46 samples, including rheumatoid arthritis patients (n = 28) healthy donors 18), with assay demonstrating sensitivity specificity 96.4% 88.9%, Indeed, results were consistent those electrochemiluminescence immunoassays digital radiography images. Overall, shows great potential for application offers universal template label-free immunoassay.

Language: Английский

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Cu2+-encapsulated DNA nanosphere and filter enable sensitive and rapid analysis of miRNA-155

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 274, P. 117203 - 117203

Published: Jan. 28, 2025

Language: Английский

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A Cascaded Chip for the High-Purity Capture and Distinguishing Detection of Phenotypic Circulating Tumor Cells in Colon Cancer

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 11, 2025

The low abundance, complex phenotypes, and need for sophisticated blood preprocessing pose substantial obstacles to the clinical implementation of circulating tumor cells (CTCs). Herein, we constructed a cascaded PMMA chip-based platform separation CTCs from other within samples, as well distinguishing detection epithelial mesenchymal CTCs. primary physical chip (PS-Chip) focused sorted whole via Dean flow fractionation (DFF) according size differences between cells, being capable eliminating approximately 93.7% red (RBCs) 68.4% white (WBCs) while maintaining CTC recovery rate around 90%. Subsequently, further purify isolated in upstream, partitioned immunoaffinity capture (PICD-Chip) featuring with two independent chambers (Zone 1, Zone 2) was designed, each which premodified Gel-GO/E/V-Apt complexes that specifically recognize distinct enabling residual upstream isolation. Upon subsequent introduction probes, namely EpCAM vimentin aptamer-modified mesoporous Pt nanoparticles (mPtNPs/E/V-Apt), into 1 2, respectively, heterogeneous ranging 5 200/mL captured were distinguished quantified utilizing exceptional peroxidase activity mPtNPs. integrated approach efficient enrichment differentiation phenotypic under requirement high purity has enabled successful application diagnosis colon cancer patients at different stages.

Language: Английский

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Ag⁺-Mediated DNA Nanomachine Cascade Nanomaterial Amplification Enable One-Pot Electrochemical Analysis of Circulating Tumor DNA

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 18, 2025

Circular tumor DNA (ctDNA) is a trace nucleic acid that functions as an essential marker. In this context, the present study proposes one-pot electrochemical analysis of ctDNA EGFR L858R in lung cancer leveraging Ag+-mediated nanosphere (I amplification) and cation exchange reaction (II amplification), Cu2+ acts signal molecule. Once target exists, it specifically destroys structure nanosphere@Ag+, large amounts Ag+ are released. After addition copper sulfide nanoparticles, can be replaced by reaction. Eventually, elevated. The analytical performance method satisfactory, detected linear range 1 aM-1 fM with detection limit 0.3 aM. Furthermore, system exhibits notable selectivity differentiating base mismatch targets other sequences. recovery rate blood samples between 95.5 105%. results from 42 clinical consistent those quantitative real-time polymerase chain reaction, computed tomography, pathology results. summary, novel strategy utilizes preprepared functional nanomaterials cascade amplification, which expected to contribute sensitive expeditious acids.

Language: Английский

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Target-Triggered Enzymatic Cascade LF-NMR Biosensor for the Detection of Circulating Tumor Cells

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

A target-triggered, enzymatic cascade-amplified low-field nuclear magnetic resonance (LF-NMR) sensor was developed for the detection of circulating tumor cell (CTC) A549. multifunctional two-dimensional bionanomaterial GDA@GOX&DNA1 designed as initiator, with Fe3O4@DNA2/Apt recognition unit and CaO2@MnO2 signal unit. When A549 present, aptamer (Apt) detached from unit, allowing formation GDA@GOX&DNA1-DNA2@Fe3O4 triggering following reactions: (1) glucose oxidase (GOX) catalyzed reaction between substrate oxygen (O2) to produce gluconic acid hydrogen peroxide (H2O2); (2) generated H2O2 reacted MnO2, producing probes Mn2+ O2; (3) CaO2 acid, generating H2O2. These cyclic reactions brought generation massive a decrease transverse relaxation time (T2), resulting in LF-NMR biosensing CTCs. Under optimal experimental conditions, linear range limit (LOD) were 10–1.0 × 106 6 cells/mL, respectively. The feasibility reliability practical applications verified by using spiked whole blood samples containing cells. This study represents first successful demonstration an biosensor intact CTCs, providing new tool clinical testing diagnosis.

Language: Английский

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Dual-mode photoelectrochemical/colorimetric sensor for CTCs detection based on seaurchin-like LaNiCo/Pd nanozymes and Exo-DNAzyme cascade program

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137837 - 137837

Published: April 1, 2025

Language: Английский

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