
Heliyon, Journal Year: 2024, Volume and Issue: 10(18), P. e38057 - e38057
Published: Sept. 1, 2024
Language: Английский
Heliyon, Journal Year: 2024, Volume and Issue: 10(18), P. e38057 - e38057
Published: Sept. 1, 2024
Language: Английский
Chemistry of Materials, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 23, 2025
Language: Английский
Citations
2Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(35), P. 14283 - 14290
Published: Aug. 23, 2024
Innovative signal amplification and transduction play pivotal roles in bioanalysis. Herein, cascading CRISPR/Cas the nanozyme are integrated with electronic an organic photoelectrochemical transistor (OPECT) to enable triple amplification, which is exemplified by miRNA-triggered CRISPR/Cas13a system polyoxometalate for OPECT detection of miRNA-21. The CRISPR/Cas13a-enabled release glucose oxidase could synergize peroxidase-like SiW12 induce catalytic precipitation on photogate, inhibiting interfacial mass transfer thus significant suppression channel current. as-developed sensor demonstrates good sensitivity selectivity miRNA-21 detection, a linear range from 1 fM 10 nM ultralow limit 0.53 fM. This study features integration bio- nanoenzyme cascade detection.
Language: Английский
Citations
9ACS Materials Au, Journal Year: 2025, Volume and Issue: 5(2), P. 377 - 384
Published: Jan. 31, 2025
Single-atom nanozymes (SANs) are a class of with metal centers that mimic the structure metalloenzymes. Herein, we report synthesis Zn–N–C SAN, which mimics action natural carbonic anhydrase enzyme. The two-step annealing technique led to content more than 18 wt %. Since act as active sites, this high loading resulted in superior catalytic activity. Zn-SAN showed CO2 uptake 2.3 mmol/g and final conversion bicarbonate 91%. was converted via biomimetic process by allowing its adsorption catalyst, followed addition catalyst HEPES buffer (pH = 8) start into HCO3–. Afterward, CaCl2 added form white CaCO3 precipitate, then filtered, dried, weighed. Active carbon MCM-41 were used controls under same reaction conditions. According findings, sequestration capacity 42 mg CaCO3/mg Zn-SAN. Some amino acids (AAs) binding affinity for Zn able suppress enzymatic activity blocking centers. This strategy detection His, Cys, Glu, Asp limits 0.011, 0.031, 0.029, 0.062 μM, respectively, hence utilized quantifying these AAs commercial dietary supplements.
Language: Английский
Citations
1Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: March 19, 2025
The wash-free method represents a promising strategy for enhancing the detection efficiency of automated chemiluminescent (CL) immunoassay analyzers. Herein, novel separation-free and was developed first time based on distance-driven CL technique. In immunoassay, well-designed Au–Co metal nanoclusters (NCs) exhibited excellent peroxidase-like activity good stability, allowing efficient catalysis luminol or its analogue (ABEI)-H2O2 system even at low concentrations. Furthermore, large specific surface area NCs facilitated accommodation greater number antibodies, thereby capture antigens achieving dual amplification signal. technique relied formation sandwich-type immunocomplexes. Upon generation hydroxyl radicals superoxide anion through catalytic decomposition H2O2 by NCs, ABEI within immunocomplexes could efficiently react with these radicals, leading to significant enhancement in signals. C-reactive protein (CRP) chosen as model analyte evaluate practicability proposed immunoassay. Notably, presented high sensitivity, selectivity, reproducibility, successfully determining CRP serum samples recoveries 96.55–106.29%. Accordingly, advantage separation-free, wash-free, reliable characteristics drastically simplify operation steps enhance efficiency, which would make advances revolution traditional
Language: Английский
Citations
1Nano Today, Journal Year: 2025, Volume and Issue: 62, P. 102739 - 102739
Published: April 1, 2025
Language: Английский
Citations
1Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown
Published: Oct. 10, 2024
The World Health Organization has warned that without effective action, deaths from drug-resistant bacteria can exceed 10 million annually, making it the leading cause of death. Conventional antibiotics are becoming less due to rapid bacterial drug resistance and slowed new antibiotic development, necessitating strategies. Recently, materials with catalytic/enzymatic properties, known as nanozymes, have been developed, inspired by natural enzymes essential for eradication. Unlike recent literature reviews broadly cover nanozyme design biomedical applications, this review focuses on latest advancements in nanozymes combating resistance, emphasizing their design, structural characteristics, applications combination therapy, future prospects. This approach aims promote development especially towards clinical translation.
Language: Английский
Citations
5Accounts of Materials Research, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 22, 2024
Language: Английский
Citations
3IntechOpen eBooks, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 27, 2025
Protein-based biomaterials are increasingly recognized for their pivotal role in tissue engineering and regenerative medicine due to superior biocompatibility, biodegradability, bioresorbability. This chapter explores the development application of protein-based that enhance cell proliferation, adhesion, integration with native tissues support regeneration. The present book outlines different types proteins used as elucidates mechanisms action By utilizing advanced fabrication techniques such electrospinning, 3D bioprinting, encapsulation adsorption methods, biomimetic scaffolds replicate extracellular matrix (ECM) can be created delivery systems therapeutic stability efficacy developed. In addition, addresses critical challenges associated extensive defects, offering promising advancements fields medicine.
Language: Английский
Citations
0Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2025, Volume and Issue: 333, P. 125894 - 125894
Published: Feb. 11, 2025
Language: Английский
Citations
0Microchimica Acta, Journal Year: 2025, Volume and Issue: 192(3)
Published: March 1, 2025
Language: Английский
Citations
0