In Vivo Screening of Barcoded Gold Nanoparticles Elucidates the Influence of Shapes for Tumor Targeting DOI
Xu-Dong Huang, Zhicheng Le, M. Ba

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 24, 2024

Abstract Gold nanoparticles (NPs) are promising for cancer therapy due to their versatile shapes, optical properties, adjustable sizes, and facile functionalization. However, diverse physicochemical complex in vivo environments, cellular heterogeneity lead markedly different interactions with cells, impacting reproducibility application selection. To address this, we implemented a DNA barcoding system label gold NPs varying surface modifications tumor delivery optimization. Six NP species, comprising three shapes (sphere, rod, triangle) two sizes (40 nm, 80 nm), were tagged unique barcodes. Barcodes demonstrated minimal detachment, no interference cell interactions, resistance DNase digestion. Next‐generation sequencing revealed that sphere exhibited poor vitro uptake but superior targeting, likely enhanced endothelial reduced macrophage clearance. Additionally, nm nanotriangles displayed excellent targeting both vivo. Supporting experiments validated these findings. demonstrate practical utility, the applied siRNA photothermal breast model. This study pioneers integration of systematically explore shape, size, delivery.

Language: Английский

Nano-Encapsulation and Conjugation Applied in the Development of Lipid Nanoparticles Delivering Nucleic Acid Materials to Enable Gene Therapies DOI Creative Commons
Linh Đinh, Lanesa Mahon, Bingfang Yan

et al.

Applied Nano, Journal Year: 2024, Volume and Issue: 5(3), P. 143 - 161

Published: Aug. 29, 2024

Nano-encapsulation and conjugation are the main strategies employed for drug delivery. Nanoparticles help improve encapsulation targeting efficiency, thus optimizing therapeutic efficacy. Through nanoparticle technology, replacement of a defective gene or delivery new into patient’s genome has become possible. Lipid nanoparticles (LNPs) loaded with genetic materials designed to be delivered specific target sites enable therapy. The lipid shells protect fragile from degradation, then successfully release payload inside cells, where it can integrate subsequently express protein interest. This review focuses on development LNPs nano-pharmaceutical techniques improving potency therapies, reducing toxicities, releasing achieve effects. In addition, we discuss preparation techniques, effects efficacy in delivering nucleic acid materials.

Language: Английский

Citations

8
Lipid nanoparticles for nucleic acid delivery beyond the liver DOI

Nadine Saber,

Mariona Estapé Sentí,

Raymond M. Schiffelers

et al.

Human Gene Therapy, Journal Year: 2024, Volume and Issue: 35(17-18), P. 617 - 627

Published: Aug. 14, 2024

Lipid nanoparticles (LNPs) are the most clinically advanced drug delivery system for nucleic acid therapeutics, exemplified by success of COVID-19 mRNA vaccines. However, their clinical use is currently limited to hepatic diseases and vaccines due tendency accumulate in liver upon intravenous administration. To fully leverage potential, it essential understand address tropism, while also developing strategies enhance tissues beyond liver. Ensuring that these therapeutics reach target cells avoiding off-target both efficacy safety. There three potential targeting strategies-passive, active, endogenous-which can be used individually or combination nonhepatic tissues. In this review, we delve into recent advancements LNP engineering delivering

Language: Английский

Citations

6
Peptide-Functionalized Lipid Nanoparticles for Targeted Systemic mRNA Delivery to the Brain DOI
Emily L. Han, Sophia Tang, Dongyoon Kim

et al.

Nano Letters, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Systemic delivery of large nucleic acids, such as mRNA, to the brain remains challenging in part due blood-brain barrier (BBB) and tendency vehicles accumulate liver. Here, we design a peptide-functionalized lipid nanoparticle (LNP) platform for targeted mRNA brain. We utilize click chemistry functionalize LNPs with peptides that target receptors overexpressed on endothelial cells neurons, namely RVG29, T7, AP2, mApoE peptides. evaluate effect LNP targeting neuronal cell transfection vitro, investigating factors serum protein adsorption, intracellular trafficking, transcytosis, exosome secretion. Finally, show peptide functionalization enhances mouse reduces hepatic after systemic administration. Specifically, RVG29 improved vivo, establishing its potential nonviral delivering

Language: Английский

Citations

6
Improving the Efficacy of Cancer mRNA Vaccines DOI
Ameya R. Kirtane, Giovanni Traverso

The Cancer Journal, Journal Year: 2025, Volume and Issue: 31(2)

Published: March 1, 2025

mRNA vaccines consist of antigen-encoding mRNA, which produces the antigenic protein upon translation. Coupling antigen production with innate immune activation can generate a potent, antigen-specific T-cell response. Clinical reports have demonstrated ability to elicit an anticancer response against various tumor types. Here, we discuss strategies enhance potency vaccines. We provide overview existing knowledge regarding and trafficking mechanisms share optimization boost mRNA-mediated production. In addition, address methods target dendritic cells lymph nodes, key initiators Finally, review for enhancing using adjuvants compatible offer unique advantages that be utilized oncology applications. However, significant work is needed understand their underlying develop technologies improve effectiveness.

Language: Английский

Citations

0
Dimethylamino-based synthetic lipidoid nanoparticles for selective mRNA delivery to splenic antigen-presenting cells DOI
Liang Xue, Chenchen Zhang, Qi Yin

et al.

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: unknown, P. 113737 - 113737

Published: April 1, 2025

Language: Английский

Citations

0
Design Considerations for Organ-Selective Nanoparticles DOI
Min‐Jun Baek, Won Hur, Satoshi Kashiwagi

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

Nanoparticles (NPs) have been extensively researched for targeted diagnostic imaging and drug delivery, yet their clinical translation remains limited, with only a few achieving Food Drug Administration approval. This limited success is primarily due to challenges in precise organ- or tissue-specific targeting, which arise from off-target tissue accumulation suboptimal clearance profiles. Herein we examine the critical role of physicochemical properties, including size, surface charge, shape, elasticity, hardness, density, governing biodistribution, targetability, NPs. We highlight recent advancements engineering NPs showcasing both significant progress remaining field nanomedicine. Additionally, discuss emerging tools technologies that are being developed address these challenges. Based on insights materials science, biomedical engineering, computational biology, research, propose key design considerations next-generation nanomedicines enhanced organ selectivity.

Language: Английский

Citations

0
Innovation of Lipid‐Based Nanoparticles: the Design and Applications in the Cardiovascular Disease Management DOI Open Access
Zhengying Gu, Tianbao Liu,

Wutian Rao

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 35(2)

Published: Oct. 9, 2024

Abstract Cardiovascular diseases (CVDs) represent a prominent cause of global mortality, posing considerable challenges for traditional therapies due to their limited bioavailability and notable side effects. Similarly, imaging diagnostic modalities have inherent limitations. Lipid‐based nanoparticles (LNPs) emerged as promising tools precise diagnosis treatment CVDs, owing exceptional biocompatibility, modifiability, compatibility with payloads, showcasing extensive potential diverse applications. LNPs excel in encapsulating drug compounds, protecting against degradation, enabling targeted delivery at lesion sites. Moreover, conjunction multimodal techniques, enhance the accuracy diagnosing treating CVDs. Nevertheless, this field lacks comprehensive up‐to‐date reviews. Therefore, review delineates foundational principles governing nano‐strategies employing LNP systems addressing It explores recent advancements utilizing these strategies different cardiovascular conditions while also pointing out This aims provide insights future research endeavors.

Language: Английский

Citations

1
In Vivo Screening of Barcoded Gold Nanoparticles Elucidates the Influence of Shapes for Tumor Targeting DOI
Xu-Dong Huang, Zhicheng Le, M. Ba

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 24, 2024

Abstract Gold nanoparticles (NPs) are promising for cancer therapy due to their versatile shapes, optical properties, adjustable sizes, and facile functionalization. However, diverse physicochemical complex in vivo environments, cellular heterogeneity lead markedly different interactions with cells, impacting reproducibility application selection. To address this, we implemented a DNA barcoding system label gold NPs varying surface modifications tumor delivery optimization. Six NP species, comprising three shapes (sphere, rod, triangle) two sizes (40 nm, 80 nm), were tagged unique barcodes. Barcodes demonstrated minimal detachment, no interference cell interactions, resistance DNase digestion. Next‐generation sequencing revealed that sphere exhibited poor vitro uptake but superior targeting, likely enhanced endothelial reduced macrophage clearance. Additionally, nm nanotriangles displayed excellent targeting both vivo. Supporting experiments validated these findings. demonstrate practical utility, the applied siRNA photothermal breast model. This study pioneers integration of systematically explore shape, size, delivery.

Language: Английский

Citations

1