Dynamic non-coding RNA biomarker reveals lung injury and repair induced by polystyrene nanoplastics

Environment International, Journal Year: 2025, Volume and Issue: 195, P. 109266 - 109266

Published: Jan. 1, 2025

Polystyrene nanoplastics (PS-NPs) are omnipresent in the air and can be inhaled by humans. However, their long-term adverse implications toxicological mechanisms for human respiratory health unclear. Therefore, this study aims to provide new insights into pulmonary toxicity of PS-NPs using mice organoid models. After subacute subchronic inhalation PS-NPs, showed pronounced lung injury characterized rate changes, altered hematology, histological evidence tissue damage oxidative stress. Similarly, repeated exposure also restricted growth cause damage. Notably, through BisqueRNA analysis a single-cell dataset canonical markers verification, it was found that induced emergence accumulation transitional cells, suggesting impaired alveolar epithelial repair processes. Sequencing analyses revealed dynamic alterations non-coding RNA (ncRNA) profiles, including circRNAs lncRNAs, response exposure. Moreover, temporal profiling highlighted distinct sets ncRNAs as early progression-associated biomarkers PS-NP-induced injury. These correlated with aberrant implicating roles disrupted cellular differen tiation mechanisms. Overall, observed multifaceted responses system, emphasizing critical involvement mediating PS-NP-induce which crucial elucidating pathophysiology nanoplastic-induced developing targeted therapeutic strategies.

Language: Английский

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HIF-1α/HO-1-Mediated Ferroptosis Participates in Polystyrene Nanoplastics-Induced Intergenerational Cardiotoxicity

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

To explore the intergenerational cardiotoxicity of nanoplastics, maternal mice were exposed to 60 nm polystyrene nanoplastics (PS-NP) during pregnancy and lactation. The results showed that PS-NP can enter hearts offspring induce myocardial fiber arrangement disorder, acidophilic degeneration cardiomyocytes, elevated creatine kinase isoenzymes (CK-MB) lactate dehydrogenase (LDH) levels after exposure at 100 mg/kg Mechanistically, KEGG analysis RNA sequencing participation hypoxia-inducible factor-1 (HIF-1) ferroptosis in PS-NP-induced cardiotoxicity. Key features ferroptosis, including Fe2+ accumulation, mitochondrial injury, oxidative stress, GPX4 downregulation, FTH1, ACSL4, SLC7A11 upregulation, detected. Furthermore, treatment upregulated expressions HIF-1α HO-1, be alleviated by inhibition using si-HIF-1α. This study provided an insightful reference for assessment PS-NP.

Language: Английский

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Intratracheal Administration of Nanoplastics With Varying Surface Hydrophobicity Results in Coarsely Vacuolated Alveolar Macrophages, Transient Respiratory Inflammation, and Mild Collagen Deposition

Nano Select, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

ABSTRACT Inhalation of biopersistent nanoplastics may have adverse effects on lung health. By varying the acetate content poly(vinyl acetate‐co‐alcohol) (PVAc), model with different surface hydrophobicity can be produced to study nanoplastic in lung. PVAc a high hydrophobicity, administered by oropharyngeal aspiration C57BL/6j mice (300 µg; ∼10 mg/kg), show transient pulmonary inflammation which peaks at 24 h post‐administration and resolves day 7. Hydrophilic induces no inflammatory same dose. Pulmonary administration hydrophilic hydrophobic increases prevalence (∼30%–35%) distinctive coarsely vacuolated alveolar macrophages over 28 days. Hydrophobic silica nanoparticles (control) induce minor collagen deposition, but do not stimulate tissue remodeling extent as bleomycin fibrosis. Longitudinal micro‐CT imaging is explored non‐invasive methodology for detection A bespoke image analysis method quantify density signal volume correlates moderately well histopathology‐derived deposition data ( R 2 = 0.73). In summary, inhaled following single 300 µg, an increase mild deposition.

Language: Английский

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Organ-Specific Effects of Polystyrene Nanoplastics on Deltamethrin-Induced Toxicity in Mice: Mitigated Hepatorenal Oxidative Damage But Increased Enteric Toxicity

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: 73(9), P. 5092 - 5101

Published: Feb. 24, 2025

This study investigates the combined effects of polystyrene nanoplastics (PS-NPs) and deltamethrin (DEL) on mice, focusing their different impacts among organs. Mice were exposed to PS-NPs and/or DEL for 30 days. Results showed that alleviated DEL-induced oxidative damage in liver kidney by reducing its accumulation due decreased bioaccessibility. Conversely, increased intestine, leading enhanced susceptibility enteric infections caused bacteria, viruses, parasites, as indicated transcriptomic analysis. delayed excretion gastrointestinal motility, evidenced altered neurotransmitter levels, thereby contributing greater intestinal DEL. Moreover, 16S rDNA sequencing revealed tended decrease beneficial bacteria increase pathogenic gut microbiota, further heightening upon coexposure. The findings this shed new light complex health risks associated with coexposure pesticides.

Language: Английский

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Occurrence, analysis, and toxicity of polyethylene terephthalate microplastics: a review

Environmental Chemistry Letters, Journal Year: 2025, Volume and Issue: unknown

Published: April 5, 2025

Language: Английский

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IL-11-Engineered Macrophage Membrane-Coated Reactive Oxygen Species-Responsive Nanoparticles for Targeted Delivery of Doxorubicin to Osteosarcoma

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 3, 2024

Osteosarcoma (OS) is a lethal malignant orthotopic bone tumor that primarily affects children and adolescents. Biomimetic nanocarriers have attracted wide attention as new strategy for delivering chemotherapy agents to the OS. However, challenges such rapid clearance limited targeting hinder effectiveness of OS chemotherapy. In this study, we designed reactive oxygen species (ROS)-responsive nanoparticles (NPs) coated with an interleukin (IL)11-engineered macrophage membrane (MM). The camouflage by MMs prevents IL-11-engineered MM-coated NPs loaded doxorubicin (IL-11/MM@NPs/Dox) immune system. Moreover, combined surface-expressed IL-11 not only directed IL-11/MM@NPs/Dox tissues but also selectively identified receptor alpha (IL-11Rα)-enriched cells. Within these cells, elevated levels ROS triggered controlled release Dox from ROS-responsive NPs. synergistic modification targeted ligand conjugation cell coating on enhanced drug availability reduced toxic side effects, thereby boosting efficacy summary, our findings suggest represents promising approach improving while ensuring excellent biocompatibility.

Language: Английский

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Contribution of Cancer-Specific Protein Coronas to the Pro-Tumor Effects of Nanoplastics through Enhanced Cellular Interactions

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(47), P. 65540 - 65552

Published: Nov. 14, 2024

The potential impact of nanoplastics (NPs) on human carcinogenic processes is a matter growing concern, particularly in light the global plastic pollution crisis. Although effects NPs health have been well investigated, many uncertainties remain regarding their role tumor behavior. Upon exposure, can enter bloodstream and are prone to interacting with plasma proteins form protein corona (PC), which influence interactions cancer cells. However, how PCs adsorbed affect particle-to-tumor cell interaction effect biological behavior unclear. To better understand formation following exposure under various clinical conditions, we investigated PC compositions derived from thyroid (TC) patients healthy volunteers. Our data revealed significant enrichment fibrinogen formed TC patient plasma, turn accelerated endocytosis into In addition, uptake pathway cells differed substantially between two groups studied due different individuals. Moreover, intriguingly alterations induced by pathology status were also found promote engulfment macrophages, resulting potent pro-inflammatory effects, exerting pro-tumor effects. These findings emphasize importance considering significance realistic identity pinpoint implications carcinogenesis outcomes humans.

Language: Английский

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THE EFFECT OF NANOPLASTICS AND MICROPLASTICS ON LUNG MORPHOLOGY AND PHYSIOLOGY: A SYSTEMATIC REVIEW

The Medical and Ecological Problems, Journal Year: 2024, Volume and Issue: 28(3), P. 42 - 60

Published: Dec. 30, 2024

Airborne microplastic (NP) and nanoplastic (MP) pollution has emerged as a pressing environmental concern with significant implications for human health. While MPs are present both indoors outdoors, indoor concentrations generally higher due to the abrasion of household materials, furniture, other domestic sources. NPs, when inhaled, accumulate in various lung regions, exerting toxic effects potentially leading respiratory diseases. This systematic review, conducted according PRISMA guidelines, evaluates recent experimental studies on pathogenic impact nanomicroplastics (NMPs) tissues using vivo vitro models. The review included 10 analyzing MP accumulation 90 examining pathogenetic mechanisms exposure. Research data indicates predominance polymeric fibers such polypropylene, polyethylene terephthalate, polytetrafluoroethylene, accounting nearly half structures. NMPs exhibit size- composition-dependent accumulation, smaller positively charged particles showing translocation potential systemic circulation organs. highlights synergistic heavy metals pollutants, interaction pulmonary surfactant, role exacerbating Current analysis underscores growing interest NMP-related health risks identifies knowledge gaps, including need standardization NMP toxicity testing further exploration biological systems. findings emphasize importance mitigating exposure safeguard pave way future research long-term impacts airborne

Language: Английский

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