Journal of Translational Medicine,
Journal Year:
2024,
Volume and Issue:
22(1)
Published: Nov. 14, 2024
To
investigate
the
potential
of
DNA
nanoparticles
(DNPs)
as
carriers
and
adjuvants
for
mRNA
vaccines.
Customized
oligonucleotides
were
assembled
into
tetrahedra
(DNA-TH),
which
subsequently
complexed
with
streptavidin
encoding
green
fluorescent
protein
(GFP).
Various
assays
conducted
to
evaluat
stability
DNPs,
their
cellular
uptake,
immune
activation
potential,
GFP
transcription
efficiency.
P53-mutant
HSC-3
cells
used
establish
a
subcutaneous
xenograft
tumor
model
explore
effects
DNPs
in
disease
model.
The
remained
stable
extracellularly
rapidly
taken
up
by
antigen-presenting
cells.
Compared
naked
mRNA,
statistically
significantly
activated
responses
facilitated
expression
both
vitro
vivo.
Immunization
DNP-GFP
complexes
induced
higher
antibody
titers
compared
mRNA.
demonstrated
good
biocompatibility.
DNP-p53
inhibited
growth
tumors
mice
p53-mutant
cells,
outperforming
p53
blank
control
groups,
significant
difference
(P
<
0.05).
show
promise
improving
vaccine
delivery
efficacy.
Further
optimization
these
could
lead
highly
effective
broad
applications.
Biomolecules,
Journal Year:
2024,
Volume and Issue:
14(12), P. 1485 - 1485
Published: Nov. 22, 2024
Hedgehog
(Hh)
signaling
is
a
well-established
developmental
pathway;
it
crucial
for
early
embryogenesis,
cell
differentiation,
and
damage-driven
regeneration.
It
being
increasingly
recognized
that
dysregulated
Hh
also
involved
in
fibrotic
diseases,
which
are
characterized
by
excessive
extracellular
matrix
deposition
compromises
tissue
architecture
function.
As
in-depth
insights
into
the
mechanisms
of
obtained,
its
complex
involvement
fibrosis
gradually
illuminated.
Notably,
some
Hh-targeted
inhibitors
currently
under
exploration
preclinical
clinical
trials
as
means
to
prevent
progression.
In
this
review,
we
provide
concise
overview
biological
signaling.
We
summarize
latest
advances
our
understanding
roles
fibrogenesis
across
liver,
kidneys,
airways,
lungs,
well
other
tissues
organs,
with
an
emphasis
on
both
shared
features
and,
more
critically,
distinct
functional
variations
observed
these
organs.
thus
highlight
context
dependence
signaling,
discuss
current
status
challenges
therapies
fibrosis.
Antioxidants,
Journal Year:
2024,
Volume and Issue:
13(12), P. 1584 - 1584
Published: Dec. 23, 2024
In
regenerative
medicine,
mesenchymal
stem
cells
(MSCs)
have
shown
their
importance
and
potential
in
tissue
reconstruction
immune
system
modification.
However,
such
cells’
is
often
diminished
by
factors
as
oxidative
stress,
rejection,
inadequate
engraftment.
This
review
highlights
the
role
of
molecular
hydrogen
(H2)
cold
atmospheric
plasma
(CAP)
adjunct
therapies
to
improve
effectiveness
MSC
therapy.
H2
has
strong
antioxidative
anti-inflammatory
actions
it
quenches
reactive
oxygen
species
positively
stimulates
Nrf2
pathway
that
promotes
survival
life.
CAP,
being
a
modulated
source
ROS
RNS,
also
assists
MSCs
altering
cellular
redox
balance,
thus
facilitating
adaptation,
migration,
differentiation.
CAP
conjunction
with
each
other
assist
establishing
an
ambience
favorable
for
promoting
MSCs’
growth
abilities,
reduce
healing
time
various
pathways
wound,
neuroprotection,
ischemia.
Besides
these
concerns,
this
covers
best
administration
routes
doses
together
study
informs
on
novel
dual
method
aimed
at
improving
outcome
therapy
while
adding
several
targets
relevant
clinical
uses
concerning
therapies.
Research
future
deal
bettering
protocols
so
therapeutic
benefits
can
be
maximized
without
long-term
implications
applications.
