Combined Effect of Nanoparticles of Silver and Silica to HeLa Cells: Synergistic Internalization and Toxicity

Environmental Toxicology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

ABSTRACT The wide range of applications and the enormous production nanomaterials have raised possibility that humans may simultaneously contact with various through multiple routes. Although numerous toxicity studies been conducted on nanomaterials, knowledge combined remains limited. Herein, toxic effects two types most widely used silver silica, were studied HeLa cells. In addition, considering there possible interplay between nanoparticles different sizes, different‐sized silica (SNPs) used. results indicate compared individual exposure, exposure to 35 nm (Ag35) 40 or 120 SNPs (SNP40 SNP120) at non‐toxic concentrations causes more severe cytotoxicity, manifested by ROS overgeneration, decreased mitochondrial membrane potential ATP level, increased apoptosis/necrosis. internalized Ag35 its dissolved Ag ions are delivered into cells adsorbing identified as primary contributors toxicity. cytotoxicity mixed SNP40 is comparable SNP120, noticeable differences in their intracellular contents subcellular locations due size effects. This study provides in‐depth insights inorganic highlights importance effect nanotoxicity assessment.

Language: Английский

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Passing the Parcels: Intercellular Nanoplastics Transfer in Mussels Perna viridis with Activated Immunomodulation

Environmental Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Nanoplastics (NPs) are generally considered to have a defined intracellular fate, being difficult excrete or transport due their stability. This study provides the first evidence of NPs intercellular transfer in hemocytes green mussels (Perna viridis), which subsequently activated immunomodulation process. were predominantly internalized by granulocytes, with portion translocated and deposited lysosomes, whereas those retained endosomes transferred new (mainly granulocytes). The direction was driven NP concentration gradients. Transfer kinetics size-dependent, smaller-sized exhibiting greater potential but lower rate, primarily specific extracellular vesicle-mediated pathway. Tunneling nanotubes provided most efficient pathway for NPs, as continuous membrane structure allowed direct substance exchange. Crucially, redistribution accompanied gradient-driven mitochondria injured hemocytes. process alleviated stress on overburdened regulated reactive oxygen species production, enhancing phagocytic activity promoting immune responses. These findings underscore that exhibit far more active behavior system than previously understood provide insights into how cells maintain health marine organisms face challenges.

Language: Английский

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Spatiotemporal Mapping of the Evolution of Silver Nanoparticles in Living Cells

ACS Nano, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

Bioaccumulated silver nanoparticles (AgNPs) can undergo transformation and release toxic Ag

Language: Английский

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Enhancement of Triple-Negative Breast Cancer-Specific Induction of Cell Death by Silver Nanoparticles by Combined Treatment with Proteotoxic Stress Response Inhibitors

Nanomaterials, Journal Year: 2024, Volume and Issue: 14(19), P. 1564 - 1564

Published: Sept. 27, 2024

Metal nanoparticles have been tested for therapeutic and imaging applications in pre-clinical models of cancer, but fears toxicity limited their translation. An emerging concept nanomedicine is to exploit the inherent drug-like properties unmodified nanomaterials cancer therapy. To be useful clinically, there must a window between nanomaterial normal cells. This necessitates identification specific vulnerabilities cancers that can targeted using without inducing off-target toxicity. Previous studies point proteotoxic stress as driver silver nanoparticle (AgNPs) Two key cell responses involved mitigating proteotoxicity are heat shock response (HSR) integrated (ISR). Here, we examine role these play AgNP-induced cytotoxicity triple-negative breast (TNBC) immortalized mammary epithelial Furthermore, investigate HSR ISR inhibitors potential drug partners increase anti-cancer efficacy AgNPs increasing We showed did not strongly induce at transcriptional level, instead decreased expression proteins (HSPs) protein possibly due degradation AgNP-treated TNBC further inhibitor, KRIBB11, synergized with cells, also increased In contrast, found salubrinal, sustain pro-death signaling, enhanced death cells Subsequent co-culture demonstrated combination salubrinal selectively eliminated TNBCs affecting grown same well. Our findings provide additional support mechanism by which kill will help guide future efforts identify would beneficial use

Language: Английский

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Manipulating Silver Nanoparticles with Biomolecular Corona Secreted from Vertebrates to Improve the Loading Capacity and Biocompatibility

ACS Nano, Journal Year: 2024, Volume and Issue: 18(42), P. 28782 - 28792

Published: Oct. 7, 2024

Silver nanoparticles (AgNPs) are widely used as nanoagents in biomedical fields, while it is still challenging to improve their loading capacity and biocompatibility microcarrier delivering systems. Herein, the physicochemical properties of AgNPs were manipulated by forming biomolecular corona derived from bovine serum albumin (AC), three organisms at various trophic levels:

Language: Английский

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