Motor protein-assisted glycan translocation and sequencing using nanopores DOI Open Access
Zhaobing Gao, Wenjun Ke, Bingqing Xia

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 29, 2024

The glycan sequencing remains a significant bottleneck in glycoscience. While nanopore platforms have achieved substantial progress single-molecule nucleic acid sequencing, their application to has faced considerable challenges, with limited advancements date. In this study, we propose novel strategy for controlling translocation through the MspA as an initial step toward sequencing. By conjugating target helicase-controlled single-stranded DNA, successfully reads of up eleven glycans. For first time, isolated glycan-associated electrical signals, enabling translocation, stretching, and controlled speed neutral glycans nanopore. This method provides platform obtaining read lengths identifying different modifications, demonstrating capability resolve monosaccharide composition glycosidic linkages. To further improve resolution, engineered M2-MspA reduce pore constriction size enhance precision by minimizing random thermal motion translocating glycan. These modifications are expected increase accuracy reliability. work represents proof-of-concept demonstration chain lays promising foundation development fingerprinting technologies. We anticipate that approach will significantly advance commercialization nanopore-based techniques.

Language: Английский

Pioneering Role of Nanopore Single-Molecule Sensing in Environmental and Food Surveillance DOI Creative Commons

W. W. Tian,

Xu Wang, Yan Zhang

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(1), P. 41 - 41

Published: Jan. 13, 2025

In recent years, environmental and food safety have garnered substantial focus due to their intimate connection with human health. Numerous biosensors been developed for identifying deleterious compounds; however, these reveal certain limitations. Nanopore sensors, featuring nano-scaled pore size, demonstrated outstanding performance in terms of rapidity, sensitivity, selectivity as a single-molecule technique surveillance. this review, we present comprehensive overview nanopore applications two fields. To elucidate the pioneering roles nanopores, analytes are categorized into three distinct groups, including metal ions, synthetic contaminants, biotoxins. Moreover, variety strategies involved, such coalescence ligand probes, implementation chemical reactions, functionalization etc. These scientific studies showcase versatility diversity technique, paving way further developments technology safety.

Language: Английский

Citations

1
Enhancing RBP4 Protein Detection in Clinical Urine Samples with Solid-State Nanopores through Optimized Sandwich Immunoassay Techniques DOI
Daqi Chen,

Zhuobin Zhu,

Wenjie Guo

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 278, P. 117318 - 117318

Published: Feb. 27, 2025

Language: Английский

Citations

0
Nanopore toward Genuine Single-Molecule Sensing: Molecular Ping-Pong Technology DOI
Xinjia Zhao, Yahui Zhang, Guangyan Qing

et al.

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 26, 2025

Nanopore sensing is a so-called label-free, single-molecule technology; however, multiple events of different molecules are recorded to obtain statistically robust data, which can limit both efficiency and sample use. To overcome these challenges, nanopore molecular ping-pong technology enables precise manipulation, reducing systematic stochastic errors by repeatedly measuring the same molecule. This review introduces fundamentals advancements technology, highlighting recent breakthrough achieving over 10,000 recaptures single dsDNA molecule within minutes. innovation not only minimizes requirements, critical for nonamplifiable samples, but also significantly enhances experimental precision. While current applications focus on dsDNA, extending this protein glycan analysis could transform research. Just as revolutionized DNA sequencing, it holds potential drive development nanopore-based sequencers, paving way groundbreaking in biology biomedicine.

Language: Английский

Citations

0
Probing Nanopores: Molecular Dynamics Insights into the Mechanisms of DNA and Protein Translocation through Solid-State and Biological Nanopores DOI

Yuan‐Shuo Zhang,

Mingming Ding

Soft Matter, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Nanopore sequencing technology has revolutionized single-molecule analysis through its unique capability to detect and characterize individual biomolecules with unprecedented precision.

Language: Английский

Citations

0
Motor protein-assisted glycan translocation and sequencing using nanopores DOI Open Access
Zhaobing Gao, Wenjun Ke, Bingqing Xia

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 29, 2024

The glycan sequencing remains a significant bottleneck in glycoscience. While nanopore platforms have achieved substantial progress single-molecule nucleic acid sequencing, their application to has faced considerable challenges, with limited advancements date. In this study, we propose novel strategy for controlling translocation through the MspA as an initial step toward sequencing. By conjugating target helicase-controlled single-stranded DNA, successfully reads of up eleven glycans. For first time, isolated glycan-associated electrical signals, enabling translocation, stretching, and controlled speed neutral glycans nanopore. This method provides platform obtaining read lengths identifying different modifications, demonstrating capability resolve monosaccharide composition glycosidic linkages. To further improve resolution, engineered M2-MspA reduce pore constriction size enhance precision by minimizing random thermal motion translocating glycan. These modifications are expected increase accuracy reliability. work represents proof-of-concept demonstration chain lays promising foundation development fingerprinting technologies. We anticipate that approach will significantly advance commercialization nanopore-based techniques.

Language: Английский

Citations

0