Redox Biology, Journal Year: 2025, Volume and Issue: unknown, P. 103689 - 103689
Published: May 1, 2025
Language: Английский
Cell Death and Disease, Journal Year: 2025, Volume and Issue: 16(1)
Published: March 6, 2025
Protein acetylation modification plays important roles in various aspects of tumor progression. Ferroptosis driven by lethal lipid peroxidation is closely related to development. Targeting ferroptosis has become a promising strategy. However, the crosstalk between protein and remains unclear. In present study, we found that acyl-CoA synthase long-chain family member 4 (ACSL4) enhances its stability double-edged sword regulation nasopharyngeal carcinoma (NPC). On one hand, ACSL4 could promote malignant progress tumors; on other it enhanced radiosensitivity endowing NPC cells with ferroptosis-sensitive properties vitro vivo. Mechanistically, histone acetyltransferase 1 (HAT1) directly promotes at lysine 383, deacetylase sirtuin 3 (SIRT3) mediates deacetylation ACSL4. Meanwhile, another 2 (HDAC2) through inhibiting transcription SIRT3. Acetylation inhibits F-box 10 (FBXO10)-mediated K48-linked ubiquitination, resulting This study reveals novel regulatory mechanism ferroptosis-related from perspective acetylation, provides method for NPC.
Language: Английский
Citations
1
Molecular Cancer, Journal Year: 2025, Volume and Issue: 24(1)
Published: March 6, 2025
Circular RNAs (circRNAs), characterized by their covalently closed-loop structures, constitute a distinct class of non-coding RNAs. They play pivotal regulatory roles within cells and are intricately associated with the progression malignant tumors. However, underlying mechanisms in nasopharyngeal carcinoma (NPC) have yet to be fully uncovered comprehensively understood. Employing RNA sequencing technology, high-abundance circular NPC were identified. Expression analysis circCLASP2 tissues was conducted using quantitative real-time polymerase chain reaction (qRT-PCR) situ hybridization experiments. Through vitro vivo functional assays, influence on proliferation metastasis investigated. LC-MS/MS technology analyzed binding partners circCLASP2, its differentially regulated targets, proteins PCMT1. Interactions among DHX9 protein, PCMT1 mRNA elucidated through immunoprecipitation pull-down techniques. The effects G-quadruplex (rG4) structures translation explored immunofluorescence (IF), ribosomal gradient separation, dual-luciferase reporter assays. Immunoprecipitation (IP) revealed downstream effector circCLASP2-DHX9-PCMT1 axis Phalloidin staining confirmed ultimate effect cytoskeleton. PDS treatment applied for interventions NPC, demonstrating potential therapeutic avenues. Our research that novel circRNA has not been reported tumors, is upregulated fosters cell both vivo. Mechanistically, acts as molecular scaffold, facilitating approximation mRNA. unwinds inhibitory rG4 structure near initiation site mRNA, increasing expression. binds upregulates cytoskeleton-associated proteins, modulating cytoskeleton strength dynamics ultimately driving metastasis. In experiments, significantly inhibits growth metastasis, showcasing promising potential. investigation pinpointed RNA, which augments cytoskeletal functions via DHX9-PCMT1 axis, contributing malignancy NPC. This pathway holds promise target Furthermore, these molecules could also serve biomarkers adjunct diagnosis prognosis assessment
Language: Английский
Citations
0
International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 308, P. 142650 - 142650
Published: March 30, 2025
Language: Английский
Citations
0
Redox Biology, Journal Year: 2025, Volume and Issue: unknown, P. 103689 - 103689
Published: May 1, 2025
Language: Английский
Citations
0