Dynamic and Diverse Coacervate Architectures by Controlled Demembranization DOI
Yang Zhou, Manfred F. Maitz, Kehu Zhang

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

The dynamics of membranes are integral to regulating biological pathways in living systems, particularly mediating intra- and extracellular communication between membraneless membranized nano- microcompartments. Mimicking these using biomimetic cell structures deepens our understanding biologically driven processes, including morphological transformations, communication, molecular sequestration within distinct environments (e.g., (membraneless) organelles, cytoplasm, cells, the matrix). In this context, demembranization coacervates represents a promising approach endow them with additional functionalities dynamic reconfiguration capabilities response external or stimuli. This versatility broadens their applicability synthetic biology, systems biotechnology. Here, we present strategy for controlled coacervate droplets. created by coating terpolymer-based nanoparticles form solid-like membrane. addition an anionic polysaccharide then triggers process arising from electrostatic competition membrane components, resulting polysaccharide-containing demembranized membranization/demembranization not only allows structural entities but also varies permeability toward (biological) (macro)molecules microscale objects. Additionally, integrating polymersome layer facilitates creation bilayer ″Janus-like″ coacervates, advancing development protocells hierarchical asymmetric structures. Our work highlights control over both membranization processes protocells, establishing platform creating advanced protein-containing diverse (membrane(less)) architectures.

Language: Английский

Cross-Linked Graphene Oxide Nanosheets with Enhanced Compensatory Charges for Ion-Exclusion Nanochannel Membranes DOI
Wenlong Liu, Qianqian Lan,

Leyao Liu

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Dynamic and Diverse Coacervate Architectures by Controlled Demembranization DOI
Yang Zhou, Manfred F. Maitz, Kehu Zhang

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

The dynamics of membranes are integral to regulating biological pathways in living systems, particularly mediating intra- and extracellular communication between membraneless membranized nano- microcompartments. Mimicking these using biomimetic cell structures deepens our understanding biologically driven processes, including morphological transformations, communication, molecular sequestration within distinct environments (e.g., (membraneless) organelles, cytoplasm, cells, the matrix). In this context, demembranization coacervates represents a promising approach endow them with additional functionalities dynamic reconfiguration capabilities response external or stimuli. This versatility broadens their applicability synthetic biology, systems biotechnology. Here, we present strategy for controlled coacervate droplets. created by coating terpolymer-based nanoparticles form solid-like membrane. addition an anionic polysaccharide then triggers process arising from electrostatic competition membrane components, resulting polysaccharide-containing demembranized membranization/demembranization not only allows structural entities but also varies permeability toward (biological) (macro)molecules microscale objects. Additionally, integrating polymersome layer facilitates creation bilayer ″Janus-like″ coacervates, advancing development protocells hierarchical asymmetric structures. Our work highlights control over both membranization processes protocells, establishing platform creating advanced protein-containing diverse (membrane(less)) architectures.

Language: Английский

Citations

0