Insights into nanoparticle surface bonding and coating architecture via multinuclear NMR DOI

Jacob D. Aubrey,

James M. Gibson, John T. Leman

et al.

Published: May 29, 2025

Tantalum oxide nanoparticles (TaOx NPs) are promising as high-Z-contrast agents for computed tomography (CT) due to their profound imaging benefits relative those of clinical iodinated contrast media (ICM) at the X-ray tube voltages ≥100 kVp required most patients. Furthermore, TaOx NPs have prevailed through extensive non-GLP and GLP (good laboratory practice) preclinical development, including in vivo/vitro safety testing efficacy studies. This is part innovative structural engineering NPs’ core size coating, which has been shown provide favorable pharmacokinetics promote rapid renal clearance, with negligible organ retention. In this study, a carboxybetaine zwitterionic siloxane polymer (CZ) coating lead candidate NP thoroughly characterized using multinuclear/multidimensional nuclear magnetic resonance (NMR) spectroscopic techniques. 1H 1H/13C heteronuclear multiple bond correlation NMR spectroscopies used confirm CZ coating’s structure, combination 29Si NMR, architecture bound surface described. Of particular significance, spectra were identify T-region bonding modes show superiority diafiltration over dialysis purification NPs. Through spectral comparison, cyclic impurity product purified was found be absent diafiltration. Finally, Carr–Purcell–Meiboom–Gill (CPMG) pulse sequence novel manner probe distance-dependent interactions between spins surface.

Language: Английский

Ultrashort Peptides as Stabilizing Agents for Colloidal Nanogold DOI
Juan David Figueroa, Sergio D. García Schejtman,

Ryan Tu

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

Ultrashort peptides hold immense potential as structural tools for enhancing the colloidal stability of nanomaterials, such nanogold. However, applications have been largely unexplored in part due to inherent complexity designing, synthesizing, and testing short nanoparticle stabilizers. In this work, we use a motif-function-guided process peptide synthesis high throughput screening evaluate spherical nanogold solutions pentapeptides. We successfully built library capable stabilizing at concentrations ≤1.0 μM. This represents 50–100-fold reduction concentration required compared other small molecules used capping agents, which illustrates using sequences Our findings could significantly impact future development high-affinity surface modifiers custom engineering by providing deeper understanding complex interactions between nanoparticles peptides.

Language: Английский

Citations

0
Advances in Ultrasmall Inorganic Nanoparticles for Nanomedicine: From Diagnosis to Therapeutics DOI
Jun Yong Oh,

Kathleen Elizabeth Villaseñor,

Andrea C Kian

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: May 9, 2025

Inorganic nanoparticles possess unique physicochemical properties that make them attractive candidates for diverse applications in nanomedicine, including as contrast agents and therapeutics. However, many inorganic are composed of high-atomic-number elements, raising safety concerns due to potential long-term retention the body. ultrasmall (UINPs), i.e., those less than ∼5 nm diameter, can offer advantage rapid renal clearance from body, reducing toxicity risks associated with prolonged exposure thereby creating a path toward clinical translation. In this review, we discuss current knowledge on design functionalization UINPs, exploring their capabilities diagnosis therapeutics, examples radiosensitization, photothermal, anti-inflammatory catalytic therapies. addition, limitations, approaches taken solve progress UINPs Through discussion, aim provide comprehensive perspective how advancing field underscoring significantly improve bioimaging therapeutic outcomes.

Language: Английский

Citations

0
Insights into nanoparticle surface bonding and coating architecture via multinuclear NMR DOI

Jacob D. Aubrey,

James M. Gibson, John T. Leman

et al.

Published: May 29, 2025

Tantalum oxide nanoparticles (TaOx NPs) are promising as high-Z-contrast agents for computed tomography (CT) due to their profound imaging benefits relative those of clinical iodinated contrast media (ICM) at the X-ray tube voltages ≥100 kVp required most patients. Furthermore, TaOx NPs have prevailed through extensive non-GLP and GLP (good laboratory practice) preclinical development, including in vivo/vitro safety testing efficacy studies. This is part innovative structural engineering NPs’ core size coating, which has been shown provide favorable pharmacokinetics promote rapid renal clearance, with negligible organ retention. In this study, a carboxybetaine zwitterionic siloxane polymer (CZ) coating lead candidate NP thoroughly characterized using multinuclear/multidimensional nuclear magnetic resonance (NMR) spectroscopic techniques. 1H 1H/13C heteronuclear multiple bond correlation NMR spectroscopies used confirm CZ coating’s structure, combination 29Si NMR, architecture bound surface described. Of particular significance, spectra were identify T-region bonding modes show superiority diafiltration over dialysis purification NPs. Through spectral comparison, cyclic impurity product purified was found be absent diafiltration. Finally, Carr–Purcell–Meiboom–Gill (CPMG) pulse sequence novel manner probe distance-dependent interactions between spins surface.

Language: Английский

Citations

0