Langmuir,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 23, 2024
This
study
investigates
the
effect
of
different
linkers
and
solvents
on
immobilization
DNA
probes
graphene
surfaces,
which
are
crucial
for
developing
high-performance
biosensors.
Quartz
crystal
microbalance
with
dissipation
(QCM-D)
measurements
were
used
to
characterize
in
situ
real-time
ssDNA
hybridization
efficiency
model
surfaces.
The
kinetics
thermodynamics
systematically
investigated
all
pairings
between
three
bifunctional
linkers─1-pyrenebutyric
acid
succinimidyl
ester
(PBSE),
Fluorenylmethylsuccinimidyl
carbonate
(FSC),
Acridine
Orange
(AO)
ester─and
organic
(DMF,
DMSO,
10%
DMF/ethanol).
linker's
spatial
orientation
effective
surface
modification
probe
attachment
also
evaluated
based
footprints
coverage.
Graphene
surfaces
functionalized
PBSE
DMF
achieved
highest
density
(up
1.31
×
10
ACS Nano,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 26, 2025
Breast
cancer
has
become
the
most
prevalent
form
of
among
women
on
a
global
scale.
The
early
and
timely
diagnosis
breast
is
utmost
importance
for
improving
survival
rate
patients
with
this
disease.
occurrence
typically
accompanied
by
dysregulation
multiple
microRNA
(miRNA)
expression
profiles.
Consequently,
simultaneous
detection
miRNAs
vital
accurate
cancer.
In
study,
bipolar
self-powered
sensor
was
developed
miRNA-451
miRNA-145
biomarkers
based
specific
catalytic
properties
enzymes.
Selenides
microporous
hollow
cubic
structure
were
designed
prepared,
which
can
markedly
enhance
enzyme
load
activity,
as
well
sensitivity,
due
to
their
extensive
surface
area
three-dimensional
porous
channel.
platform
integrated
into
commercial
chip,
signal
presented
in
smartphone
interface,
thereby
enabling
real-time
continuous
monitoring.
Furthermore,
machine
learning
utilized
predict
miRNA
detection,
encompasses
numerous
stages,
including
data
collection,
feature
extraction,
model
training,
validation.
comparison
limited
sensing
efficiency
biosensors
driven
biofuel
cells,
our
achieved
quantitative
analysis
targets,
providing
robust
tool
more
comprehensive
understanding
function
its
association
cancers.
Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 30, 2025
The
development
of
highly
sensitive
detection
methods
for
bioanalysis
is
crucial
early
disease
diagnosis.
Electrochemical
biosensing
technology
offers
unique
advantages
in
this
area
due
to
its
rapid
response,
high
sensitivity,
and
low
cost.
However,
achieving
efficient
transfer
signaling
molecules
the
electrode
interface
facilitate
effective
interaction
between
signal
sensing
surface
remains
a
critical
challenge
ultrasensitive
electrochemical
detection.
In
study,
we
discovered
that
single-stranded
DNA-modified
magnetic
nanoprobes
(signal
probe
A)
subjected
cryogenic
treatment
can
rapidly
form
an
orderly
monolayer
at
under
external
field,
while
phenomenon
was
not
observed
with
double-stranded
B).
Building
on
finding,
developed
protective
complementary
strand
B)
that,
upon
target
molecules,
converted
into
A.
This
transformation,
combined
treatment,
enables
molecules.
Using
miRNA-21
carcinoembryonic
antigen
(CEA)
as
model
targets,
optimized
conditions,
limit
3.4
aM
0.28
fg/mL
CEA
excellent
versatility.
summary,
study
introduces
efficient,
rapid,
enzyme-free,
environmentally
friendly
amplification
strategy.
approach
only
provides
innovative
solution
but
also
new
insights
enhancing
molecule–sensor
interactions
biosensors.
Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 22, 2025
Creating
biosensors
capable
of
facilely
and
entirely
excluding
the
influence
interfering
biomolecules
in
complex
samples
holds
profound
significance
for
advancing
detection
technology
diagnostics.
Here,
we
develop
a
wireless
portable
biosensor
(WPB)
that
prevents
interference
from
abundant
serum
through
homogeneous
hybridization
S9.6
antibody-mediated
multivalent
capture.
By
transferring
environment
heterogeneous
chip
surface
to
solution,
maintains
consistent
efficiency
as
buffer.
Additionally,
use
capture
ensures
nearly
unchanged
binding
affinity
compared
On
basis
multiple
enhancements,
WPB
can
achieve
ultrasensitive
nucleic
acid
50%
human
serum.
Specifically,
subtle
blocker
is
designed
eliminate
competitive
monovalent
antibody-heteroduplex
binding,
ensuring
interactions.
The
also
enables
single-step
detection.
Moreover,
sensing
platform
utilizes
interferents
situ
antifouling
prevent
nonspecific
adsorption.
As
result,
proposed
achieves
similar
limit
acids
(95
aM)
buffer
(86
aM).
This
approach
inspires
new
idea
elimination
usage
exhibits
comprehensive
performance
with
potential
future
diagnostic
applications.
Critical Reviews in Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown, P. 1 - 12
Published: April 29, 2025
Apolipoprotein-A4
(Apo-A4)
is
a
plasma
protein
that
plays
role
in
various
physiological
and
behavioral-emotional
reactions
when
faced
with
stress.
Studies
have
shown
close
relationship
between
Apo-A4
the
onset
of
depression
its
symptoms.
However,
there
currently
no
reliable
laboratory
approach
to
confirm
diagnosis
depression.
Therefore,
development
precise
effective
technique
assess
might
help
early
detection
screening
other
related
psychiatric
diseases,
as
well
tracking
managing
course
treatment.
As
technology
advances,
biosensors
become
quick,
accurate,
sensitive
tools
for
personal
care
illness
diagnosis.
Biosensors
measuring
detecting
levels
recently
been
designed.
These
studies
emphasized
accurate
diagnostic
measurement
techniques.
This
review
attempts
give
general
overview
introduce
established
measurement.
