Multidimensional exploration of hydrogels as biological scaffolds for spinal cord regeneration: mechanisms and future perspectives DOI Creative Commons
Cheng Han, Jiao Jiao, Chan Gong

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: April 23, 2025

Spinal cord injury (SCI) is a severe condition that frequently leads to permanent disabilities and neurological dysfunction. Its progression driven by multifaceted pathophysiology, encompassing direct trauma, secondary cascades, intricate cellular molecular responses. While current therapies focus on alleviating symptoms restoring functionality, achieving effective neural regeneration in the spinal continues be significant challenge. Hydrogels, recognized for their exceptional biocompatibility, conductivity, injectability, have shown great potential as advanced scaffolds support neuronal axonal regeneration. Recently, these materials attracted interest field of SCI rehabilitation research. This review concludes recent progress hydrogel-based strategies rehabilitation, emphasizing distinct properties, underlying mechanisms, integration with bioactive molecules, stem cells, complementary biomaterials. Hydrogels foster providing tailored microenvironment, while features such self-repair, electrical controlled drug release significantly enhance therapeutic experimental models. explores hydrogel technologies applications, underscoring address challenges treatment paving way future clinical implementation.

Language: Английский

Phase-adapted Metal Ion Supply for Spinal Cord Repair with a Mg-Zn Incorporated Chimeric Microsphere DOI
Xiangyu Liu,

Biao Ma,

S L Hu

et al.

Biomaterials, Journal Year: 2025, Volume and Issue: 320, P. 123253 - 123253

Published: March 11, 2025

Language: Английский

Citations

0
Single-Cell RNA Sequencing Revealed Functional Conjunctival Keratinocytes Loss via TGF-β-Wnt/β-Catenin Signaling in Sjögren’s Syndrome Related Dry Eye DOI Creative Commons
Bowen Wang, Lihong Liang, Hao Zeng

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2025, Volume and Issue: 66(4), P. 43 - 43

Published: April 16, 2025

The role of the conjunctiva in pathophysiology Sjögren's syndrome (SS) related dry eye disease (DED) remains obscure especially view functional conjunctival epithelia. In order to illustrate effects SS, we investigated interactions between parenchymal cells and immune with single-cell RNA sequencing technique. Freshly collected from a canonical SS model was prepared for 10 × Genomics T cell receptor (TCR) sequencing. Conjunctiva Western blot, immunofluorescence, multiplex immunohistochemical (mIHC), flow cytometry. Phenol red thread test, lissamine staining, qRT-PCR were applied evaluate signs DED. DED phenotype validated model. Loss water-secreting keratinocyte projected scRNA-seq data proved by mIHC test mice. proportion Lgr4+ basal epithelial poor ability differentiate into mature increased, Wnt/β-catenin signaling upregulated it under regulation TGF-β derived macrophages. Such macrophages promoted angiogenesis through secretion VEGFA activate endothelial cells. Immuno-fibroblasts had an increased population, which implicated specifically activated chemotaxis. conjunctiva, TGF-β-Wnt/β-catenin axis downregulated formation keratinocytes accompanied infiltration pro-angiogenetic pro-fibrotic macrophage pro-inflammatory cell.

Language: Английский

Citations

0
Regulation of Neuroimmune Microenvironment by PLA/GO/Anti-TNF-α Composite to Enhance Neurological Repair After Spinal Cord Injury DOI Creative Commons
Yishu Liu, Jingsong Liu, Yangyang Wang

et al.

International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 4919 - 4942

Published: April 1, 2025

Spinal cord injury (SCI) is a severe neurological condition with limited treatment options. Polylactic acid (PLA)+graphene oxide (GO)+anti-TNF-α (Ab) composites have shown potential in regulating immune responses and promoting neural repair. Electrospinning PLA+GO+Ab materials were characterized using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), X-ray photoelectron (XPS), Raman spectroscopy, diffraction (XRD). Their effects on stem cells (NSCs) macrophage polarization evaluated through vitro assays, including proliferation, migration, differentiation, flow cytometry. A rat SCI model was used to assess motor function recovery histological changes. promoted NSC differentiation while inducing toward the M2 phenotype, reducing inflammation. In model, enhanced recovery, reduced spinal damage, axonal regeneration oligodendrocyte maturation. RNA sequencing identified activation of Rap1 signaling pathway, contributing these effects. effectively modulate neuroimmune microenvironment, supporting by repair regulation. These findings suggest its as therapeutic biomaterial for treatment.

Language: Английский

Citations

0
Multidimensional exploration of hydrogels as biological scaffolds for spinal cord regeneration: mechanisms and future perspectives DOI Creative Commons
Cheng Han, Jiao Jiao, Chan Gong

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: April 23, 2025

Spinal cord injury (SCI) is a severe condition that frequently leads to permanent disabilities and neurological dysfunction. Its progression driven by multifaceted pathophysiology, encompassing direct trauma, secondary cascades, intricate cellular molecular responses. While current therapies focus on alleviating symptoms restoring functionality, achieving effective neural regeneration in the spinal continues be significant challenge. Hydrogels, recognized for their exceptional biocompatibility, conductivity, injectability, have shown great potential as advanced scaffolds support neuronal axonal regeneration. Recently, these materials attracted interest field of SCI rehabilitation research. This review concludes recent progress hydrogel-based strategies rehabilitation, emphasizing distinct properties, underlying mechanisms, integration with bioactive molecules, stem cells, complementary biomaterials. Hydrogels foster providing tailored microenvironment, while features such self-repair, electrical controlled drug release significantly enhance therapeutic experimental models. explores hydrogel technologies applications, underscoring address challenges treatment paving way future clinical implementation.

Language: Английский

Citations

0