Multiplexed Detection of Pancreatic-Specific Nucleic Acids and Protein Biomarkers Using a Logic Nanofluidic Platform DOI
Lina Wang, Yuanyuan Jia, Jin Wang

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 19, 2025

Early detection of pancreatic cancer is vital for patient survival. However, current diagnostic approaches remain constrained by insufficient precision and specificity inherent to single-biomarker strategies. Herein, we develop a nanochannel biosensing platform implementing cooperative dual-signal pancreatic-specific biomarkers CA19-9 miRNA-196a. Using liquid-liquid interface self-assembly, constructed anodic aluminum oxide (AAO)-Au hybrid nanochannels integrated with surface-modified double-key DNA nanolock (DDN). The conformational switch DDN logic gating triggered miRNA-196a exposes the CA19-9-aptamer, enabling specific target recognition consequent ion signal attenuation. Simultaneously, released quantified catalytic hairpin assembly hybridization chain reaction-mediated cascade amplification. Experiments show that present DDN-based nanofluidic could achieve an ultralow limit 0.000027 U·mL-1 4.74 aM miRNA-196a, which 2-3 orders magnitude higher than traditional ELISA/qPCR methods. Finally, clinical sample analysis confirms high this in distinguishing acute pancreatitis from healthy individuals. This DDN-functionalized biosensor provides valuable insights into designing platforms cancer, highlighting its significant potential diagnostics.

Language: Английский

Multiplexed Detection of Pancreatic-Specific Nucleic Acids and Protein Biomarkers Using a Logic Nanofluidic Platform DOI
Lina Wang, Yuanyuan Jia, Jin Wang

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 19, 2025

Early detection of pancreatic cancer is vital for patient survival. However, current diagnostic approaches remain constrained by insufficient precision and specificity inherent to single-biomarker strategies. Herein, we develop a nanochannel biosensing platform implementing cooperative dual-signal pancreatic-specific biomarkers CA19-9 miRNA-196a. Using liquid-liquid interface self-assembly, constructed anodic aluminum oxide (AAO)-Au hybrid nanochannels integrated with surface-modified double-key DNA nanolock (DDN). The conformational switch DDN logic gating triggered miRNA-196a exposes the CA19-9-aptamer, enabling specific target recognition consequent ion signal attenuation. Simultaneously, released quantified catalytic hairpin assembly hybridization chain reaction-mediated cascade amplification. Experiments show that present DDN-based nanofluidic could achieve an ultralow limit 0.000027 U·mL-1 4.74 aM miRNA-196a, which 2-3 orders magnitude higher than traditional ELISA/qPCR methods. Finally, clinical sample analysis confirms high this in distinguishing acute pancreatitis from healthy individuals. This DDN-functionalized biosensor provides valuable insights into designing platforms cancer, highlighting its significant potential diagnostics.

Language: Английский

Citations

0