Describing the complex chemistry of benthic seawater: from exometabolite sampling strategies to MS-based metabolomics
Natural Product Reports,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
This
review
highlights
advances
in
characterizing
exometabolites
(EMs)
from
benthic
organisms,
starting
with
situ
sampling
methods,
then
discussing
how
marine
MS-based
(exo)metabolomics
benefits
various
fields
while
addressing
ongoing
challenges.
Language: Английский
Circadian migrations of cave-dwelling crustaceans guided by their home chemical seascape
Frontiers in Marine Science,
Journal Year:
2024,
Volume and Issue:
11
Published: Sept. 17, 2024
Organisms
release
and
detect
molecules
for
defense,
reproduction,
feeding
strategies
finding
suitable
habitats.
For
some
migratory
species,
homing
behavior
could
be
related
to
the
recognition
of
their
home
chemical
fingerprint
made
an
assemblage
from
habitat.
In
marine
realm,
functioning
ecosystems
such
as
underwater
caves
largely
depends
on
trophic
interactions
between
outside
environment.
A
key
feature
these
relies
circadian
migration
small
crustaceans
(Mysida)
cave
habitat
open
sea.
Recently,
it
has
been
hypothesized
that
migrations
involve
mediation.
Behavioral
experiments
using
a
two-choice
system
have
shown
mysids
significantly
seawater
rather
than
control
water
Here,
we
used
same
experimental
investigate
by
two
populations
mysid
Hemimysis
margalefi
.
Both
were
submitted
choice
three
distinct
seawaters
vs.
seawater.
Additionally,
tested
preference
non-cave
species
(
Leptomysis
sp.)
seawaters.
To
evaluate
whether
was
influenced
cues
conspecifics,
complementary
experiment
H.
conducted.
Results
demonstrated
each
studied
population
recognizes
its
own
habitat,
this
is
not
occurrence
’s
exudates.
Mass
spectrometry-based
metabolomic
analyses
revealed
had
specific
with
only
few
reproducibly
detected
signals
belonging
different
classes:
peptides,
alkaloids,
fatty
acids,
steroids
but
also
inorganic
molecules.
Organic
pollutants
detected.
Among
compounds,
one
oxylipin
derivative
peptide
considered
markers
ecosystem.
Therefore,
postulate
seascape
participates
which
are
analogous
daily-based
behavior.
Language: Английский
Metabolomic signatures of pathogen suppression effect of Baltic eelgrass meadows in surrounding seawater
Caroline Utermann-Thüsing,
No information about this author
Daniel Méndez,
No information about this author
Paolo Stincone
No information about this author
et al.
The Science of The Total Environment,
Journal Year:
2025,
Volume and Issue:
979, P. 179518 - 179518
Published: April 27, 2025
Organic
molecules
exuded
into
water
column
by
marine
organisms
represent
a
significant
portion
of
dissolved
organic
matter
(DOM)
that
modulates
biochemical
interactions.
Secreted
allelochemicals
have
been
suggested
to
be
involved
in
regulation
pathogen
abundance
seagrass
meadows,
however,
exometabolome
has
remained
unstudied.
We
aimed
identify
exometabolites,
within
and
outside
explore
their
potential
involvement
suppression
under
varying
environmental
conditions.
collected
seawater
(SW)
samples
from
eelgrass
(Zostera
marina)-vegetated
(V)
non-vegetated
(NV)
areas
across
5
locations
spanning
270
km
coastline
along
the
German
Baltic
Sea.
Comparative
LC-MS/MS-based
untargeted
computational
metabolomics
combined
with
statistical
analyses
machine
learning
tools
were
employed
pinpoint
(exo)metabolomic
signatures
leaves.
Simultaneously,
we
measured
abiotic
parameters
three
common
pathogenic
taxa
seawater,
investigated
spatiotemporal
variations.
Here
show
correlation
biomass
reduction
effect
increasing
temperature,
eutrophication
anthropogenic
influences.
Exometabolomics
studies
revealed
exudates
contributed
significantly
overall
DOM
at
molecular
level,
while
SW
overlying
meadows
contained
many
chemical
features
unique
leaf
metabolome.
identified
four
flavone
aglycones
as
key
biomarkers
distinguishing
SW-V
SW-NV
samples.
Their
drastically
increased
concentrations
correlated
lowest
biomass,
suggesting
role
regulation.
These
analytical
microbiological
approaches
indicate
flavones
are
defensive
released
upon
stress
serve
bioindicators
eelgrass'
sanitation
effect.
Language: Английский
In Situ Capture and Real-Time Enrichment of Marine Chemical Diversity
ACS Central Science,
Journal Year:
2023,
Volume and Issue:
9(11), P. 2084 - 2095
Published: Nov. 8, 2023
Analyzing
the
chemical
composition
of
seawater
to
understand
its
influence
on
ecosystem
functions
is
a
long-lasting
challenge
due
inherent
complexity
and
dynamic
nature
marine
environments.
Describing
intricate
chemistry
requires
optimal
in
situ
sampling.
Here
presented
novel
underwater
hand-held
solid-phase
extraction
device,
I-SMEL
(In
Situ
Marine
moleculE
Logger),
which
aims
concentrate
diluted
molecules
from
large
volumes
delimited
zone
targeting
keystone
benthic
species.
holobionts,
such
as
sponges,
can
impact
their
surroundings
possibly
through
production
release
specialized
metabolites,
hence
termed
exometabolites
(EMs).
was
deployed
sponge-dominated
Mediterranean
at
15
m
depth.
Untargeted
MS-based
metabolomics
performed
enriched
EM
extracts
showed
(1)
diversity
metabolites
(2)
reproducible
recovery
enrichment
sponge
EMs
aerothionin,
demethylfurospongin-4,
longamide
B
methyl
ester.
These
constitute
identity
each
targeted
species:
Aplysina
cavernicola,
Spongia
officinalis,
Agelas
oroides,
respectively.
concentrated
10
L
water
min
sampling
time.
The
present
proof
concept
with
opens
new
research
perspectives
ecology
sets
stage
for
further
sustainable
efforts
natural
product
chemistry.
Language: Английский