In Situ Capture and Real-Time Enrichment of Marine Chemical Diversity DOI Creative Commons
Morgane Mauduit, Marie Derrien, Marie Grenier

et al.

ACS Central Science, Journal Year: 2023, Volume and Issue: 9(11), P. 2084 - 2095

Published: Nov. 8, 2023

Analyzing the chemical composition of seawater to understand its influence on ecosystem functions is a long-lasting challenge due inherent complexity and dynamic nature marine environments. Describing intricate chemistry requires optimal in situ sampling. Here presented novel underwater hand-held solid-phase extraction device, I-SMEL (In Situ Marine moleculE Logger), which aims concentrate diluted molecules from large volumes delimited zone targeting keystone benthic species. holobionts, such as sponges, can impact their surroundings possibly through production release specialized metabolites, hence termed exometabolites (EMs). was deployed sponge-dominated Mediterranean at 15 m depth. Untargeted MS-based metabolomics performed enriched EM extracts showed (1) diversity metabolites (2) reproducible recovery enrichment sponge EMs aerothionin, demethylfurospongin-4, longamide B methyl ester. These constitute identity each targeted species: Aplysina cavernicola, Spongia officinalis, Agelas oroides, respectively. concentrated 10 L water min sampling time. The present proof concept with opens new research perspectives ecology sets stage for further sustainable efforts natural product chemistry.

Language: Английский

Describing the complex chemistry of benthic seawater: from exometabolite sampling strategies to MS-based metabolomics DOI Creative Commons
Morgane Mauduit, Stéphane Greff, Marie Derrien

et al.

Natural Product Reports, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

This review highlights advances in characterizing exometabolites (EMs) from benthic organisms, starting with situ sampling methods, then discussing how marine MS-based (exo)metabolomics benefits various fields while addressing ongoing challenges.

Language: Английский

Citations

1
Circadian migrations of cave-dwelling crustaceans guided by their home chemical seascape DOI Creative Commons
Marie Derrien, Mathieu Santonja, Stéphane Greff

et al.

Frontiers in Marine Science, Journal Year: 2024, Volume and Issue: 11

Published: Sept. 17, 2024

Organisms release and detect molecules for defense, reproduction, feeding strategies finding suitable habitats. For some migratory species, homing behavior could be related to the recognition of their home chemical fingerprint made an assemblage from habitat. In marine realm, functioning ecosystems such as underwater caves largely depends on trophic interactions between outside environment. A key feature these relies circadian migration small crustaceans (Mysida) cave habitat open sea. Recently, it has been hypothesized that migrations involve mediation. Behavioral experiments using a two-choice system have shown mysids significantly seawater rather than control water Here, we used same experimental investigate by two populations mysid Hemimysis margalefi . Both were submitted choice three distinct seawaters vs. seawater. Additionally, tested preference non-cave species ( Leptomysis sp.) seawaters. To evaluate whether was influenced cues conspecifics, complementary experiment H. conducted. Results demonstrated each studied population recognizes its own habitat, this is not occurrence ’s exudates. Mass spectrometry-based metabolomic analyses revealed had specific with only few reproducibly detected signals belonging different classes: peptides, alkaloids, fatty acids, steroids but also inorganic molecules. Organic pollutants detected. Among compounds, one oxylipin derivative peptide considered markers ecosystem. Therefore, postulate seascape participates which are analogous daily-based behavior.

Language: Английский

Citations

5
Metabolomic signatures of pathogen suppression effect of Baltic eelgrass meadows in surrounding seawater DOI Creative Commons

Caroline Utermann-Thüsing,

Daniel Méndez,

Paolo Stincone

et al.

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 979, P. 179518 - 179518

Published: April 27, 2025

Organic molecules exuded into water column by marine organisms represent a significant portion of dissolved organic matter (DOM) that modulates biochemical interactions. Secreted allelochemicals have been suggested to be involved in regulation pathogen abundance seagrass meadows, however, exometabolome has remained unstudied. We aimed identify exometabolites, within and outside explore their potential involvement suppression under varying environmental conditions. collected seawater (SW) samples from eelgrass (Zostera marina)-vegetated (V) non-vegetated (NV) areas across 5 locations spanning 270 km coastline along the German Baltic Sea. Comparative LC-MS/MS-based untargeted computational metabolomics combined with statistical analyses machine learning tools were employed pinpoint (exo)metabolomic signatures leaves. Simultaneously, we measured abiotic parameters three common pathogenic taxa seawater, investigated spatiotemporal variations. Here show correlation biomass reduction effect increasing temperature, eutrophication anthropogenic influences. Exometabolomics studies revealed exudates contributed significantly overall DOM at molecular level, while SW overlying meadows contained many chemical features unique leaf metabolome. identified four flavone aglycones as key biomarkers distinguishing SW-V SW-NV samples. Their drastically increased concentrations correlated lowest biomass, suggesting role regulation. These analytical microbiological approaches indicate flavones are defensive released upon stress serve bioindicators eelgrass' sanitation effect.

Language: Английский

Citations

0
In Situ Capture and Real-Time Enrichment of Marine Chemical Diversity DOI Creative Commons
Morgane Mauduit, Marie Derrien, Marie Grenier

et al.

ACS Central Science, Journal Year: 2023, Volume and Issue: 9(11), P. 2084 - 2095

Published: Nov. 8, 2023

Analyzing the chemical composition of seawater to understand its influence on ecosystem functions is a long-lasting challenge due inherent complexity and dynamic nature marine environments. Describing intricate chemistry requires optimal in situ sampling. Here presented novel underwater hand-held solid-phase extraction device, I-SMEL (In Situ Marine moleculE Logger), which aims concentrate diluted molecules from large volumes delimited zone targeting keystone benthic species. holobionts, such as sponges, can impact their surroundings possibly through production release specialized metabolites, hence termed exometabolites (EMs). was deployed sponge-dominated Mediterranean at 15 m depth. Untargeted MS-based metabolomics performed enriched EM extracts showed (1) diversity metabolites (2) reproducible recovery enrichment sponge EMs aerothionin, demethylfurospongin-4, longamide B methyl ester. These constitute identity each targeted species: Aplysina cavernicola, Spongia officinalis, Agelas oroides, respectively. concentrated 10 L water min sampling time. The present proof concept with opens new research perspectives ecology sets stage for further sustainable efforts natural product chemistry.

Language: Английский

Citations

7