Deleted Journal,
Journal Year:
2023,
Volume and Issue:
1(1), P. 10002 - 10002
Published: Jan. 1, 2023
Unique
structural
features
and
wide
applications
of
gold
nanoparticles
(GNPs)
are
inspiring
researchers
to
develop
biocompatible,
reliable
cost-effective
methods
for
their
synthesis.
Herein,
a
clean,
eco-friendly
non-toxic
method
obtain
GNPs
was
developed
by
reducing
capping
the
liquid
extract
stem
Lilium
longiflorum
highlights
catalytic
reduction
4-nitrophenol
(4-NP)
methylene
blue
(MB).
The
formation
confirmed
through
absorption
peak
at
535
nm
in
UV-Vis
spectra.
TEM
HRTEM
analyses
reveal
spherical
morphology
with
an
average
size
4.97
nm.
SEM
EDX
further
elucidate
nature
elemental
composition.
FTIR
spectroscopy
analysis
demonstrates
that
were
coated
organic
compounds,
which
prevent
nanoparticle
from
aggregation.
exhibit
remarkable
efficiency
4-NP
MB.
efficacy
synthesized
demonstrated
enhanced
rates
MB,
rate
constants
1.50
min−1
1.29
min−1,
respectively.
This
study
develops
novel
technique
synthesis
opens
possibilities
green
other
metal
nanoparticles.
activity
holds
promise
range
industrial
environmental
sustainability.
Probiotics and Antimicrobial Proteins,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 26, 2025
Abstract
This
study
aimed
to
synthesize
silver
nanoparticles
(AgNPs)
using
the
postbiotic
of
Ligilactobacillus
salivarius
KC27L
strain
and
evaluate
their
multifunctional
biological
properties.
The
use
L.
,
a
probiotic
bacterium
known
for
its
ability
produce
wide
range
metabolites,
plays
crucial
role
in
this
process
by
acting
as
natural,
eco-friendly
reducing,
stabilizing
agent
during
AgNP
synthesis.
approach
not
only
eliminates
need
hazardous
chemicals
typically
used
nanoparticle
synthesis
but
also
enhances
biocompatibility
efficacy
resulting
nanoparticles.
Synthesized
AgNPs
were
analyzed
Fourier
transform
infrared
spectroscopy,
FTIR
(metabolites
postbiotic);
UV–vis
(peak
435
nm);
scanning
electron
microscope,
SEM;
transmission
microscopy,
TEM
(spherical
shapes,
sizes
<
50
nm),
energy-dispersive
spectrometry,
EDS
at
3
keV);
zeta
potential
(−
18.6
mV).
These
(0.156–40
mg/mL)
evaluated
antimicrobial
anti-biofilm
activities
against
Escherichia
coli
ATCC
11229,
Pseudomonas
aeruginosa
27853,
Staphylococcus
aureus
25923,
epidermidis
35984,
Streptococcus
mutans
25175,
antioxidant
four
different
methods
(2,2-diphenyl-1-picrylhydrazyl
free
radical
scavenging,
metal
ion
chelating,
hydroxyl
superoxide
anion
scavenging
activities).
Also,
cytotoxic
activity
was
investigated
normal
cell
line
(L929)
24,
48,
72
h.
At
concentration
40
mg/mL,
demonstrated
highest
efficacy,
with
inhibition
zones
measured
14.9
mm
P.
9.5
E.
15.7
S.
12.9
.
exhibited
all
Gram-positive
Gram-negative
bacteria
strains
studied.
According
DPPH
method,
determined
mg/mL
(80.93%).
found
have
no
toxic
effect
low
concentrations
(0.39–25
µg/mL).
Biogenic
synthesized
could
be
biotechnological
applications
(biomaterials,
health,
environmental,
etc.)
antibacterial,
anti-biofilm,
antioxidant,
nontoxic
However,
further
research
is
needed
understand
mechanisms
action
particles
fully.
Microbiology Spectrum,
Journal Year:
2024,
Volume and Issue:
12(2)
Published: Jan. 3, 2024
The
emergence
and
international
dissemination
of
multi-drug
resistant
Staphylococcus
aureus
(S.
aureus)
strains
challenge
current
antibiotic-based
therapies,
representing
an
urgent
threat
to
public
health
worldwide.
In
the
U.S.
alone,
S.
infections
are
responsible
for
11,000
deaths
500,000
hospitalizations
annually.
Biofilm
formation
is
a
major
contributor
antibiotic
tolerance
resistance-induced
delays
in
empirical
therapy
with
increased
infection
severity,
frequency,
treatment
failure,
mortality.
Developing
novel
strategies
prevent
disrupt
biofilm
imperative.
this
article,
we
test
Secretion
Modification
Region
(SMR)
peptides
inhibitory
effects
on
biofilm-forming
capacity
by
targeting
molecular
chaperone
DnaK.
dose
effect
SMR
was
assessed
using
microtiter
plate
methods
confocal
microscopy.
Interaction
between
antagonist
DnaK
determined
immune
precipitation
anti-Flag
M2
Affinity
Western
blot
analysis.
Increasing
peptide
concentrations
exhibited
increasing
blockade
significant
inhibition
found
at
18
µM,
36
72
µM.
This
work
supports
potential
therapeutic
benefit
reducing
viability
could
improve
susceptibility
antimicrobial
agents.IMPORTANCEThe
development
anti-biofilm
agents
critical
restoring
bacterial
sensitivity,
directly
combating
evolution
resistance,
overall
clinical
burden
related
pervasive
biofilm-mediated
infections.
Thus,
study,
peptide,
small
molecule
derived
from
HIV
Nef
protein,
preliminarily
explored
properties.
shown
effectively
target
inhibit
dose-dependent
manner.
These
results
support
further
investigation
into
mechanism
peptide-mediated
rational
drug
design
identification
targets.
Deleted Journal,
Journal Year:
2023,
Volume and Issue:
1(1), P. 10002 - 10002
Published: Jan. 1, 2023
Unique
structural
features
and
wide
applications
of
gold
nanoparticles
(GNPs)
are
inspiring
researchers
to
develop
biocompatible,
reliable
cost-effective
methods
for
their
synthesis.
Herein,
a
clean,
eco-friendly
non-toxic
method
obtain
GNPs
was
developed
by
reducing
capping
the
liquid
extract
stem
Lilium
longiflorum
highlights
catalytic
reduction
4-nitrophenol
(4-NP)
methylene
blue
(MB).
The
formation
confirmed
through
absorption
peak
at
535
nm
in
UV-Vis
spectra.
TEM
HRTEM
analyses
reveal
spherical
morphology
with
an
average
size
4.97
nm.
SEM
EDX
further
elucidate
nature
elemental
composition.
FTIR
spectroscopy
analysis
demonstrates
that
were
coated
organic
compounds,
which
prevent
nanoparticle
from
aggregation.
exhibit
remarkable
efficiency
4-NP
MB.
efficacy
synthesized
demonstrated
enhanced
rates
MB,
rate
constants
1.50
min−1
1.29
min−1,
respectively.
This
study
develops
novel
technique
synthesis
opens
possibilities
green
other
metal
nanoparticles.
activity
holds
promise
range
industrial
environmental
sustainability.
