Berkala Penelitian Hayati,
Journal Year:
2023,
Volume and Issue:
29(3), P. 106 - 112
Published: Dec. 30, 2023
Infectious
diseases,
including
urinary
tract
infections
(UTIs),
significantly
impact
global
health,
diminishing
the
quality
of
life
and
increasing
clinical
economic
burdens.
Concerns
arise
due
to
potential
development
antimicrobial
resistance
(AMR)
facilitated
by
biofilm
formation.
Nanotechnology,
specifically
silver
nanoparticles
(1–100
nm),
proves
effective
against
bacterial
strains,
making
them
valuable
in
biomedical
field.
Advances
microscopic
imaging
techniques,
such
as
Confocal
Laser
Scanning
Microscopy
(CLSM),
enhance
our
understanding
biofilms.
This
study
aims
assess
effectiveness
optimal
concentration
gold
combating
biofilms
formed
uropathogenic
Escherichia
coli
(UPEC)
through
CLSM
analysis.
The
research
comprises
following
stages:
rejuvenation
isolates
inoculum
preparation,
testing
anti-biofilm
activity
at
concentrations
25,
50,
75,
100
ppm
UPEC
using
findings
reveal
that
both
exhibit
ppm,
confirmed
for
is
identified
ppm.
Antibiotics,
Journal Year:
2024,
Volume and Issue:
13(4), P. 343 - 343
Published: April 9, 2024
Microbial
biofilm
formation
creates
a
persistent
and
resistant
environment
in
which
microorganisms
can
survive,
contributing
to
antibiotic
resistance
chronic
inflammatory
diseases.
Increasingly,
biofilms
are
caused
by
multi-drug
microorganisms,
which,
coupled
with
diminishing
supply
of
effective
antibiotics,
is
driving
the
search
for
new
therapies.
In
this
respect,
antimicrobial
peptides
(AMPs)
short,
hydrophobic,
amphipathic
that
show
activity
against
multidrug-resistant
bacteria
formation.
They
also
possess
broad-spectrum
diverse
mechanisms
action.
comprehensive
review,
150
publications
(from
January
2020
September
2023)
were
collected
categorized
using
terms
‘polypeptide
agent’,
‘antimicrobial
peptide’,
‘biofilm’.
During
period,
wide
range
natural
synthetic
AMPs
studied,
LL-37,
polymyxin
B,
GH12,
Nisin
most
frequently
cited.
Furthermore,
although
many
microbes
Staphylococcus
aureus
Pseudomonas
aeruginosa
popular.
Publications
considered
AMP
combinations
potential
role
delivery
systems
increasing
efficacy
AMPs,
including
nanoparticle
delivery.
Relatively
few
focused
on
resistance.
This
review
informs
guides
researchers
about
latest
developments
research,
presenting
promising
evidence
as
agents.
Biofilm,
Journal Year:
2024,
Volume and Issue:
7, P. 100187 - 100187
Published: Feb. 27, 2024
The
potential
uses
for
antibiofilm
surfaces
reach
across
different
sectors
with
significant
resultant
economic,
societal
and
health
impact.
For
those
interested
in
using
the
built
environment,
it
is
important
that
efficacy
testing
methods
are
relevant,
reproducible
standardised
where
possible,
to
ensure
data
outputs
applicable
end-use,
comparable
literature.
Using
pre-defined
keywords,
a
review
of
literature
reporting
on
antimicrobial
(78
articles),
within
which
application
was
described
as
non-submerged/non-medical
surface
or
coating
action,
undertaken.
most
used
utilized
growth
biofilm
submerged
static
systems.
Quantification
varied
(from
least
commonly
used)
colony
forming
unit
counts,
non-microscopy
fluorescence
spectroscopy,
microscopy
analysis,
direct
agar-contact,
sequencing,
ELISA.
Selection
media,
microbial
species,
incubation
temperature
also
varied.
In
many
cases,
definitions
attempts
quantify
activity
were
absent
vague.
Assessing
after
recovery
assessing
regrowth
initial
analysis
almost
entirely
absent.
It
clear
field
would
benefit
from
widely
agreed
adopted
approaches
guidance
how
select
incorporate
end-use
specific
conditions,
alongside
minimum
guidelines
may
Engineering in Life Sciences,
Journal Year:
2025,
Volume and Issue:
25(2)
Published: Feb. 1, 2025
The
enhanced
surface
cylindrical
flask
(ESCF)
consists
of
an
eight-striped
inner
arrangement
holding
16
standard
microscopic
slides
placed
inside
a
vessel.
specially
designed
spatula-accessible
can
be
withdrawn
from
the
vessel
during
cultivation
without
disturbing
biofilm
formation
through
innovative
window-flap
accessibility
mechanism.
and
its
accessories
were
three-dimensional
(3D)
printed
by
applying
fused
deposition
modeling
technique
utilizing
biodegradable
polylactic
acid.
Biofilms
clinically
relevant
bacteria
namely
Klebsiella
pneumoniae,
Pseudomonas
aeruginosa,
Staphylococcus
aureus,
Escherichia
coli
successfully
grown
in
ESCF
observed
confocal
laser
scanning
microscopy.
Advantages
device
include
area
for
formation,
ease
insertion
removal
slides,
convenient
fitting
into
rotary
shaker
platforms,
creation
anoxic/microaerophilic
environment
as
well
feasibility
pH,
dissolved
gases,
metabolite
measurements
liquid
surrounding
biofilm.
will
find
widespread
application
medical,
industrial,
environmental
disciplines.
The Analyst,
Journal Year:
2024,
Volume and Issue:
150(2), P. 318 - 328
Published: Nov. 22, 2024
A
SERS
chip
integrated
with
a
micro-chamber
array
and
nano-silver
substrate
was
fabricated
for
the
monitoring
of
pyocyanine,
overcoming
detection
shortcomings
such
as
discontinuity,
invasion,
complex
pretreatment.
