Berkala Penelitian Hayati,
Journal Year:
2023,
Volume and Issue:
29(3), P. 106 - 112
Published: Dec. 30, 2023
Infectious
diseases,
including
urinary
tract
infections
(UTIs),
significantly
impact
global
health,
diminishing
the
quality
of
life
and
increasing
clinical
economic
burdens.
Concerns
arise
due
to
potential
development
antimicrobial
resistance
(AMR)
facilitated
by
biofilm
formation.
Nanotechnology,
specifically
silver
nanoparticles
(1–100
nm),
proves
effective
against
bacterial
strains,
making
them
valuable
in
biomedical
field.
Advances
microscopic
imaging
techniques,
such
as
Confocal
Laser
Scanning
Microscopy
(CLSM),
enhance
our
understanding
biofilms.
This
study
aims
assess
effectiveness
optimal
concentration
gold
combating
biofilms
formed
uropathogenic
Escherichia
coli
(UPEC)
through
CLSM
analysis.
The
research
comprises
following
stages:
rejuvenation
isolates
inoculum
preparation,
testing
anti-biofilm
activity
at
concentrations
25,
50,
75,
100
ppm
UPEC
using
findings
reveal
that
both
exhibit
ppm,
confirmed
for
is
identified
ppm.
Berkala Penelitian Hayati,
Journal Year:
2024,
Volume and Issue:
30(1), P. 8 - 14
Published: Feb. 6, 2024
Infectious
diseases,
including
urinary
tract
infections
(UTIs),
significantly
impact
global
health,
diminishing
the
quality
of
life
and
increasing
clinical
economic
burdens.
Concerns
arise
due
to
potential
development
antimicrobial
resistance
(AMR)
facilitated
by
biofilm
formation.
Nanotechnology,
specifically
silver
nanoparticles
(1–100
nm),
proves
effective
against
bacterial
strains,
making
them
valuable
in
biomedical
field.
Advances
microscopic
imaging
techniques,
such
as
Confocal
Laser
Scanning
Microscopy
(CLSM),
enhance
our
understanding
biofilms.
This
study
aims
assess
effectiveness
optimal
concentration
gold
combating
biofilms
formed
uropathogenic
Escherichia
coli
(UPEC)
through
CLSM
analysis.
The
research
comprises
following
stages:
rejuvenation
isolates
inoculum
preparation,
testing
anti-biofilm
activity
at
concentrations
25,
50,
75,
100
ppm
UPEC
using
findings
reveal
that
both
exhibit
ppm,
confirmed
for
is
identified
ppm.
Journal of Applied Phycology,
Journal Year:
2024,
Volume and Issue:
36(6), P. 3163 - 3173
Published: Aug. 26, 2024
Abstract
Benthic
phototrophic
communities
play
a
crucial
role
in
the
functioning
of
shallow
lake
ecosystems.
The
complexity
benthic
algal
poses
challenges
for
exploring
their
fine
structures
using
traditional
methods
such
as
light
microscopy.
However,
confocal
laser
scanning
microscopy
(CLSM)
offers
potential
microscale-level
structural
investigation
biofilms,
thereby
contributing
to
deeper
understanding
and
compositional
changes
within
these
communities.
primary
objective
this
study
was
develop
relatively
rapid,
semi-automated,
freely
available
image
processing
method
facilitate
tracking
spatio-temporal
alterations
intact
biofilms.
To
evaluate
our
method,
we
conducted
an
eight-week
long
outdoor
mesocosm
experiment,
manipulating
temperatures
based
on
two
global
warming
scenarios
(assuming
intermediate-
high
degree
greenhouse
gas
emission),
with
increases
3°C
5°C,
respectively,
compared
reference/unheated
mesocosms.
Eight
limestone
cubes
were
placed
bottom
each
provide
'natural'
surfaces
phytobenthos
colonization,
which
subsequently
analysed
by
We
hypothesized
that
increasing
water
temperature
might
have
significant
impact
taxonomic
composition
particularly
abundance
prokaryotes
eukaryotes.
designed
'FIJI
macro'
efficiently
recorded
images,
including
single
series
Z-stack
projection
images.
Our
effectively
discriminated
photosynthetic
eukaryotes
pigment
proved
suitable
high-throughput
analysis
Moreover,
confirmed
climate-induced
can
lead
dominance
cyanobacteria
at
expense
eukaryotic
algae.
The
monitoring
of
signaling
molecules
related
quorum
sensing
(QS)
in
bacterial
biofilms
was
crucial
for
the
better
understanding
how
QS
influenced
biofilm
formation.
In
this
paper,
a
SERS
microfluidic
chip
integrated
with
array
micro-chambers
and
Nano-silver
substrate
fabricated
Pseudomonas
aeruginosa
biofilms.
self-assembled
showed
good
homogeneity
stability
enhancement
factor
up
to
1.84×108,
which
guaranteed
detection
sensitivity
molecules.
dynamic
change
pyocyanine,
as
an
important
signal
molecule,
monitored
during
growth
by
comparing
peak
intensity
at
1350
cm-1.
By
using
same
chip,
inhibitory
effects
different
drugs
including
conventional
antibiotics
(ceftazidime),
well
natural
antibacterial
components
(curcumin
tannic
acid)
were
also
investigated.
order
inhibition
effect
on
pseudomonas
turns
ceftazidime
>
curcumin
acid.
secretion
period
pyocyanin
affected
treatment.
Furthermore,
pyocyanine
secreted
confirmed
mass
spectrometry
(MS)
concentration
relationship
obtained
MS/MS
consistent
detection.
proposed
method
based
had
advantages
in-situ
detection,
flexibility
efficiency.
Berkala Penelitian Hayati,
Journal Year:
2023,
Volume and Issue:
29(3), P. 106 - 112
Published: Dec. 30, 2023
Infectious
diseases,
including
urinary
tract
infections
(UTIs),
significantly
impact
global
health,
diminishing
the
quality
of
life
and
increasing
clinical
economic
burdens.
Concerns
arise
due
to
potential
development
antimicrobial
resistance
(AMR)
facilitated
by
biofilm
formation.
Nanotechnology,
specifically
silver
nanoparticles
(1–100
nm),
proves
effective
against
bacterial
strains,
making
them
valuable
in
biomedical
field.
Advances
microscopic
imaging
techniques,
such
as
Confocal
Laser
Scanning
Microscopy
(CLSM),
enhance
our
understanding
biofilms.
This
study
aims
assess
effectiveness
optimal
concentration
gold
combating
biofilms
formed
uropathogenic
Escherichia
coli
(UPEC)
through
CLSM
analysis.
The
research
comprises
following
stages:
rejuvenation
isolates
inoculum
preparation,
testing
anti-biofilm
activity
at
concentrations
25,
50,
75,
100
ppm
UPEC
using
findings
reveal
that
both
exhibit
ppm,
confirmed
for
is
identified
ppm.
