Membrane-Active Amphiphilic EGCG Derivatives and Their Silver Nanoparticles-Formulation as Broad-Spectrum Antibacterial and Antibiofilm Agents DOI

Shabin N. Chathangad,

Vishnu N. Vijayan,

Nissy A. Bovas

et al.

ACS Applied Nano Materials, Journal Year: 2024, Volume and Issue: 7(16), P. 18920 - 18931

Published: Aug. 6, 2024

Eight hydroxyl groups in (−)-epigallocatechin-3-gallate (EGCG) make it difficult to access the hydrophobic domain of bacterial cell membranes and display strong antibacterial activity at physiologically attainable concentrations. Herein, we strategically modified EGCG into an amphiphilic molecule that can surpass its limitations transform a potent agent. Seven alkyl ether derivatives (4″-Cn EGCG, n = 6, 8, 10, 12, 14, 16, 18) have been designed, synthesized assessed for their efficacy mode action. 4″-C14 4″-C16 were found be most effective compounds exhibiting >99% inhibition against Escherichia coli Bacillus subtilis colony numbers concentration as low 1 μg/mL. They able induce intracellular ROS generation disrupt membrane integrity. Further, enhanced water solubility achieved by synthesizing silver nanoparticles (AgNPs) EGCG. The AgNPs characterized UV–vis, FT-IR, TEM, DLS. Compared exhibited large reduction minimum inhibitory (MIC) with significantly improved water-solubility. Notably, could eliminate drug-resistant strains such methicillin-resistant Staphylococcus aureus (>99% 100 μg/mL), Acinetobacter baumannii 10 μg/mL) Pseudomonas aeruginosa 50 indicating broad-spectrum high potential combat antimicrobial resistance. Furthermore, showed biofilm formed subtilis. MTT assay on 3T3-L1 mouse fibroblast cells revealed no significant cytotoxicity or up 150 μg/mL, selectivity toward cells. Taken together, this work demonstrates rational development exhibit antibiofilm via multimodal mechanisms.

Language: Английский

Membrane-Active Amphiphilic EGCG Derivatives and Their Silver Nanoparticles-Formulation as Broad-Spectrum Antibacterial and Antibiofilm Agents DOI

Shabin N. Chathangad,

Vishnu N. Vijayan,

Nissy A. Bovas

et al.

ACS Applied Nano Materials, Journal Year: 2024, Volume and Issue: 7(16), P. 18920 - 18931

Published: Aug. 6, 2024

Eight hydroxyl groups in (−)-epigallocatechin-3-gallate (EGCG) make it difficult to access the hydrophobic domain of bacterial cell membranes and display strong antibacterial activity at physiologically attainable concentrations. Herein, we strategically modified EGCG into an amphiphilic molecule that can surpass its limitations transform a potent agent. Seven alkyl ether derivatives (4″-Cn EGCG, n = 6, 8, 10, 12, 14, 16, 18) have been designed, synthesized assessed for their efficacy mode action. 4″-C14 4″-C16 were found be most effective compounds exhibiting >99% inhibition against Escherichia coli Bacillus subtilis colony numbers concentration as low 1 μg/mL. They able induce intracellular ROS generation disrupt membrane integrity. Further, enhanced water solubility achieved by synthesizing silver nanoparticles (AgNPs) EGCG. The AgNPs characterized UV–vis, FT-IR, TEM, DLS. Compared exhibited large reduction minimum inhibitory (MIC) with significantly improved water-solubility. Notably, could eliminate drug-resistant strains such methicillin-resistant Staphylococcus aureus (>99% 100 μg/mL), Acinetobacter baumannii 10 μg/mL) Pseudomonas aeruginosa 50 indicating broad-spectrum high potential combat antimicrobial resistance. Furthermore, showed biofilm formed subtilis. MTT assay on 3T3-L1 mouse fibroblast cells revealed no significant cytotoxicity or up 150 μg/mL, selectivity toward cells. Taken together, this work demonstrates rational development exhibit antibiofilm via multimodal mechanisms.

Language: Английский

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