The Binding of the SARS-CoV-2 Spike Protein to Platelet Factor 4: A Proposed Mechanism for the Generation of Pathogenic Antibodies DOI Creative Commons
Thi‐Huong Nguyen, Liyu Chen,

Nida Zaman Khan

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(3), P. 245 - 245

Published: Feb. 20, 2024

Pathogenic platelet factor 4 (PF4) antibodies contributed to the abnormal coagulation profiles in COVID-19 and vaccinated patients. However, mechanism of what triggers body produce these has not yet been clarified. Similar patterns many comparable features between virus heparin-induced thrombocytopenia (HIT) have reported. Previously, we identified a new autoimmunity HIT which PF4-antibodies self-clustered PF4 exposed binding epitopes for other pathogenic PF4/eparin antibodies. Here, first proved that SARS-CoV-2 spike protein (SP) also binds PF4. The was evidenced by increase mass optical intensity as observed through quartz crystal microbalance immunosorbent assay, while switching surface zeta potential caused interactions affinity PF4-SP were evaluated dynamic light scattering isothermal spectral shift analysis. Based on our results, proposed generation We further validated changes interaction SP found their differs from ACE2–SP binding. Importantly, PF4/SP complexes facilitate anti-PF4/Heparin Our findings offer fresh perspective engagement with SP, illuminating role severe thrombotic events.

Language: Английский

Basic implications on three pathways associated with SARS-CoV-2 DOI Creative Commons
Jong‐Hoon Lee, Consolato Sergi, Richard E. Kast

et al.

Biomedical Journal, Journal Year: 2024, Volume and Issue: unknown, P. 100766 - 100766

Published: July 1, 2024

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) interacts between the host and virus govern induction, resulting in multiorgan impacts. Its pathophysiology involves followings: 1) angiotensin-converting enzyme (ACE2) Toll-like receptor (TLR) pathways: 2) neuropilin (NRP) pathway: 3) spike protein pathway. Therefore, it is necessary to block pathological course with modulating innate lymphoid cells against diverse corona variants future.

Language: Английский

Citations

4
The Binding of the SARS-CoV-2 Spike Protein to Platelet Factor 4: A Proposed Mechanism for the Generation of Pathogenic Antibodies DOI Creative Commons
Thi‐Huong Nguyen, Liyu Chen,

Nida Zaman Khan

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(3), P. 245 - 245

Published: Feb. 20, 2024

Pathogenic platelet factor 4 (PF4) antibodies contributed to the abnormal coagulation profiles in COVID-19 and vaccinated patients. However, mechanism of what triggers body produce these has not yet been clarified. Similar patterns many comparable features between virus heparin-induced thrombocytopenia (HIT) have reported. Previously, we identified a new autoimmunity HIT which PF4-antibodies self-clustered PF4 exposed binding epitopes for other pathogenic PF4/eparin antibodies. Here, first proved that SARS-CoV-2 spike protein (SP) also binds PF4. The was evidenced by increase mass optical intensity as observed through quartz crystal microbalance immunosorbent assay, while switching surface zeta potential caused interactions affinity PF4-SP were evaluated dynamic light scattering isothermal spectral shift analysis. Based on our results, proposed generation We further validated changes interaction SP found their differs from ACE2–SP binding. Importantly, PF4/SP complexes facilitate anti-PF4/Heparin Our findings offer fresh perspective engagement with SP, illuminating role severe thrombotic events.

Language: Английский

Citations

0