Towards Understanding the Role of the Glycosylation of Proteins Present in Extracellular Vesicles in Urinary Tract Diseases: Contributions to Cancer and Beyond DOI Creative Commons

Magdalena Wilczak,

Magdalena Surman, Małgorzata Przybyło

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(22), P. 5241 - 5241

Published: Nov. 6, 2024

Extracellular vesicles (EVs) are a population of nanoscale particles surrounded by phospholipid bilayer, enabling intercellular transfer bioactive molecules. Once released from the parental cell, EVs can be found in most biological fluids human body and isolated them. For this reason, have significant diagnostic potential serve as an excellent source circulating disease biomarkers. Protein glycosylation plays key role many processes, aberrant is hallmark various diseases. been shown to carry multiple glycoproteins, but little known about specific roles these glycoproteins context EVs. Moreover, changes EV described for several diseases, including cancers metabolic, cardiovascular, neurological or kidney Urine richest EVs, providing almost unlimited (in terms volume) opportunities non-invasive isolation. Recent studies also revealed pathological link between urinary urological cancers, well other pathologies tract. In review, we discuss recent research advances field diagnostic/prognostic glycosylation. addition, summarize common methods isolating urine techniques used study their

Language: Английский

Fingerprint Profiling of Glycans on Extracellular Vesicles via Lectin-Induced Aggregation Strategy for Precise Cancer Diagnostics DOI
Guihua Zhang, Xiaodan Huang,

Yanli Gong

et al.

Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 5, 2024

Extracellular vesicles (EVs) harbor abundant glycans that mediate various functions, such as intercellular communication and disease advancement, which play significant roles in progression. However, the presence of EV heterogeneity body fluids complex nature glycan structures have posed challenges for detection glycans. In this study, we provide a streamlined method integrated,

Language: Английский

Citations

8
Clinical Impact of Exosome Chemistry in Cancer DOI

Shrishti Madhan,

Rajib Dhar, Arikketh Devi

et al.

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

As we progress into the 21st century, cancer stands as one of most dreaded diseases. With approximately in every four individuals facing a lifetime risk developing cancer, remains serious health challenges worldwide. Its multifaceted nature makes it an arduous and tricky problem to diagnose treat. Over years, researchers have explored plenty approaches avenues improve management. One notable strategy includes study extracellular vesicles (EVs) potential biomarkers therapeutics. Among these EVs, exosomes emerged particularly promising candidates due their unique characteristic properties functions. They are small membrane-bound secreted by cells carrying cargo biomolecules such proteins, nucleic acids, lipids. These play crucial roles intercellular communication, facilitating transfer biological information between cell-to-cell communication. Exosomes transport cargoes DNA, RNA, lipids involved cellular reprogramming promoting cancer. In this review, explore molecular composition exosomes, significance chemistry development, its theranostic application well research complications solutions.

Language: Английский

Citations

1
Self-Localized Plasmonic Nanocavity Strategy for the Glycosylation Detection of Glioblastoma Extracellular Vesicles DOI

Peilin Wang,

Zihui Liang, Wenyan Li

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

The protein glycosylation of extracellular vesicles (EVs) is involved in cellular recognition and emerges as a promising biomarker for cancer diagnosis. However, the lack efficient labeling high-resolution detection strategies limits their clinical application. Herein, we developed self-localized plasmonic nanocavity strategy to analyze characteristics glioblastoma EVs. First, an engineered phospholipid bilayer structure with Au nanoring array was designed capture EVs induce membrane fusion. Relying on multifunctional proximity process, peroxidase-induced label sialic acid programmed cell death ligand 1 (PD-L1) Based identification process EVs, nanocubes achieved spontaneous location MoSe2 QDs. uniformly enhanced electromagnetic field resulted polarized luminescence signal QDs improving sensitivity resolution. This system demonstrated precise distinction sensitive quantification EV cerebrospinal fluid distinguish glioblastoma. research provided novel promoted application

Language: Английский

Citations

1
Multiplexed glycan immunofluorescence identification of pancreatic cancer cell subpopulations in both tumor and blood samples DOI Creative Commons

Braelyn Binkowski,

Zachary Klamer, Chong Gao

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(10)

Published: March 7, 2025

Pancreatic ductal adenocarcinoma (PDAC) tumor heterogeneity impedes the development of biomarker assays for early disease detection. We hypothesized that PDAC cell subpopulations could be identified by aberrant glycan signatures in both tissue and blood samples. used multiplexed immunofluorescence to distinguish between noncancer within tissue, we developed hybrid sandwich determine whether detected found cells were glycans four glycan-binding proteins (VVL, CA19-9, sTRA, GM2) there are three types glycan-defined tumors: sTRA type, CA19-9 intermixed. In patient-matched samples, type determined blood. As a result, combined more sensitive specific than any individual assay. Our results demonstrate methodology detect stratify PDAC.

Language: Английский

Citations

1
Biosensor-based methods for exosome detection with applications to disease diagnosis DOI

Weikang Ge,

Zheying Mu,

Shiao Yang

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: unknown, P. 117362 - 117362

Published: March 1, 2025

Language: Английский

Citations

1
Multiplexed Glycan Immunofluorescence Identification of Pancreatic Cancer Cell Subpopulations in Both Tumor and Blood Samples DOI Creative Commons

Braelyn Binkowski,

Zachary Klamer, Chong Gao

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 23, 2024

Pancreatic ductal adenocarcinoma (PDAC) tumor heterogeneity impedes the development of biomarker assays suitable for early disease detection that would improve patient outcomes. The CA19-9 glycan is currently used as a standalone PDAC. Furthermore, previous studies have shown cancer cells may display aberrant membrane-associated glycans. We therefore hypothesized PDAC cell subpopulations could be distinguished by signatures. multiplexed immunofluorescence combined with pathologist annotation and automated image processing to distinguish between within tissue. Using training-set/test-set approach, we found identified signatures comprising 4 glycans (VVL, CA19-9, sTRA, GM2) there are three glycan-defined types: sTRA type, intermixed. To determine whether detected in blood samples, developed hybrid sandwich In both patient-matched proportion was consistent. our immunofluorescent approach proved more sensitive specific than alone. Our results provide proof concept novel methodology

Language: Английский

Citations

3
Comparative evaluation of methods for isolating extracellular vesicles from ICC cell culture supernatants: Insights into proteomic and glycomic analysis DOI Creative Commons
Linlin Wu, Jiao Wei, Yueping Zhan

et al.

Cell Communication and Signaling, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 29, 2025

Extracellular vesicles (EVs) are nanoscale structures involved in intercellular communication and play a key role cancer pathology. Intrahepatic cholangiocarcinoma (ICC) is highly invasive malignancy marked by abnormal sialylated glycosylation. Analyzing proteins glycans EVs provides insights into ICC molecular subtyping mechanisms. Optimizing EV isolation methods for ICC-derived enables comprehensive proteomic glycomic analysis. We systematically evaluated five methods-Ultracentrifugation (UC), exoEasy, Total Exosome Isolation (TEI), EVtrap, ÄKTA-by analyzing the biophysical properties, profiles, of EVs. Subsequently, we applied TMT-based quantitative proteome light/heavy methylamine labeling quantification N-glycan linkage isomers to investigate alterations N-glycans within secreted HuCCT1 HCCC-9810 cells with overexpressing ST6 β‑galactoside α2,6‑sialyltransferase 1 (ST6GAL1). By evaluating proteome, N-glycome extracted using different methods, UC was identified as optimal approach this study, it offered balance between operational complexity, cost-effectiveness, preservation activity. In total 1,928 high-confidence over 84 were quantified. ST6GAL1 exhibited consistent upregulation 16 proteins, downregulation 10 well 3 glycans. Quantitative analysis revealed that overexpression led significant cell adhesion glycosylation pathways, along specific changes structures. Notably, these modifications extended beyond α2,6-sialylation, suggesting interactions glycosyltransferases may drive alterations.

Language: Английский

Citations

0
Role of cancer cell-derived exosomal glycoproteins in macrophage polarization DOI
Khandu Wadhonkar,

Yashi Singh,

Aurelia Rughetti

et al.

Molecular Biology Reports, Journal Year: 2025, Volume and Issue: 52(1)

Published: May 10, 2025

Language: Английский

Citations

0
ECM1 and ANXA1 in urinary extracellular vesicles serve as biomarkers for breast cancer DOI Creative Commons
Hai Huang,

Jingyu Wan,

Xudong Ao

et al.

Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14

Published: July 8, 2024

Objective Although urinary extracellular vesicles (uEVs) have been extensively studied in various cancers, their involvement breast cancer (BC) remains largely unexplored. The non-invasive nature of urine as a biofluid and its abundant protein content offer considerable potential for the early detection cancer. Methods This study analyzed proteomic profiles uEVs from BC patients healthy controls (HC). dysregulation ECM1 ANXA1 was validated larger cohort 128 patients, 25 HC benign nodules (BBN) by chemiluminescence assay (CLIA). expression levels were also confirmed MMTV-PyMT transgenic mouse models. Results LC-MS/MS analysis identified 571 dysregulated proteins patients. selected validation BBN using CLIA, fold change showed significant difference more than 10 with p -value<0.05. Protein significantly increased In addition, mice observed to increase progressively progression Conclusion We developed simple purification-free platform isolate quantitatively detect WGA-coupled magnetic beads CLIA. Our results suggest that could potentially serve diagnostic biomarkers

Language: Английский

Citations

1
Infrared Spectrometry Enables Detection of Changes Induced by Altered N-Glycosylation in Overall Molecular Composition, Lipid Saturation, Protein Structure and Other Protein-Related Parameters in Melanoma Cells and Their Extracellular Vesicles DOI

Magdalena Wilczak,

Andrzej Wróbel, Magdalena Surman

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0