A Label‐Free Fluorescence Aptasensor for the Detection of Ciprofloxacin in Actual Samples

ChemistrySelect, Journal Year: 2025, Volume and Issue: 10(7)

Published: Feb. 1, 2025

Abstract Ciprofloxacin (CIP) is a third‐generation fluoroquinolone antibiotic widely used in veterinary and clinical settings. However, overuse can result the development of quinolone‐resistant pathogens, posing risks to ecosystems human health. In this work, we developed fluorescence method for detecting CIP based on FRET (Förster Resonance Energy Transfer) between fluorescein isothiocyanate (FITC) fluorescent dye graphene oxide (GO). The aptamer was immobilized GO surface through π–π stacking interaction preventing effectively FITC from coming into contact strong fluorescence. Upon addition CIP, combined preferentially leaded release GO, which made be close leading significant quenching due FRET. intensity exhibited consistent reduction proportion concentrations with 2.99–800 nM. Under same conditions, limit detection calculated 2.99 sensor serum, urine, milk spiked recoveries ranged 99.65%–100.97% relative standard deviations ranging 0.17%–1.49%, provided tool food safety control standards.

Language: Английский

From Traditional to Modern: Nanotechnology-Driven Innovation in Mycotoxin Sensing for Chinese Herbal Medicines

Talanta, Journal Year: 2025, Volume and Issue: 288, P. 127681 - 127681

Published: Feb. 2, 2025

Language: Английский

Development of terbium-based fluorescence probe for Vericiguat quantitation in tablets and human plasma through advanced computational approaches

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113782 - 113782

Published: April 1, 2025

Language: Английский