Peptide Nucleic Acids in Saturation Transfer Difference Nuclear Magnetic Resonance Experiments: A Simple and Valuable Tool for Studying HuR–Small Molecule Complexes DOI Creative Commons

Irene Gado,

Martina Garbagnoli,

Francesca Alessandra Ambrosio

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(45), P. 45147 - 45158

Published: Oct. 30, 2024

Ribonucleic acid (RNA)-binding proteins (RBPs) play a key role in regulating RNA stability, fate, function, gene expression, post-transcriptional modifications, and cellular activities. Among the various RBPs identified to date, Hu have been most extensively studied. Specifically, HuR influences several processes, including cell proliferation, differentiation, stress response, it is frequently overexpressed solid tumors. Several ligands so far, highlighting druggability of such protein. To discover novel HuR–RNA interfering agents, biophysical assays represent promising approach. overcome limitations for manipulation, this work, we explored use PNA (peptide nucleic acid) as an analogue interaction studies. Molecular modeling simulation revealed ability aegPNA bind and, therefore, synthesis designed was conducted. The saturation transfer difference (STD) nuclear magnetic resonance (NMR) technique adopted evaluate interfere with HuR–PNA complex, comparing obtained results RNAs. Our evidenced that may be considered simple valuable tool analyze properties by STD-NMR, thus improving precision reliability

Language: Английский

Peptide Nucleic Acids in Saturation Transfer Difference Nuclear Magnetic Resonance Experiments: A Simple and Valuable Tool for Studying HuR–Small Molecule Complexes DOI Creative Commons

Irene Gado,

Martina Garbagnoli,

Francesca Alessandra Ambrosio

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(45), P. 45147 - 45158

Published: Oct. 30, 2024

Ribonucleic acid (RNA)-binding proteins (RBPs) play a key role in regulating RNA stability, fate, function, gene expression, post-transcriptional modifications, and cellular activities. Among the various RBPs identified to date, Hu have been most extensively studied. Specifically, HuR influences several processes, including cell proliferation, differentiation, stress response, it is frequently overexpressed solid tumors. Several ligands so far, highlighting druggability of such protein. To discover novel HuR–RNA interfering agents, biophysical assays represent promising approach. overcome limitations for manipulation, this work, we explored use PNA (peptide nucleic acid) as an analogue interaction studies. Molecular modeling simulation revealed ability aegPNA bind and, therefore, synthesis designed was conducted. The saturation transfer difference (STD) nuclear magnetic resonance (NMR) technique adopted evaluate interfere with HuR–PNA complex, comparing obtained results RNAs. Our evidenced that may be considered simple valuable tool analyze properties by STD-NMR, thus improving precision reliability

Language: Английский

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