Hsa-miR-7974 Suppresses Epstein-Barr Virus Reactivation by Directly Targeting BZLF1 and BRLF1

Viruses, Journal Year: 2025, Volume and Issue: 17(5), P. 594 - 594

Published: April 23, 2025

Epstein-Barr virus (EBV) reactivation, a key factor in (EBV)-associated malignancies, is regulated by specific cellular microRNAs (miRNAs). This study investigated the role of Hsa-miR-7974 (miR-7974) this process. miRNA sequencing revealed significant downregulation miR-7974 reactivated EBV-positive cell lines (Raji and C666-1). Bioinformatics prediction dual-luciferase assays confirmed direct targeting EBV immediate-early gene BRLF1 miR-7974. Furthermore, mimics suppressed, whereas inhibitors increased, expression lytic genes (BZLF1, BRLF1, BMRF1) viral load, as validated RT-qPCR. analyses involvement pathways such membrane dynamics signal transduction (MAPK, NF-κB, IL-10), its association with Hodgkin’s lymphoma, leukemia, nasopharyngeal neoplasms. These findings establish that functions crucial negative regulator reactivation directly highlighting potential significance pathogenesis EBV-associated malignancies.

Language: Английский

Designing molecular hybrids as novel breast cancer therapeutics

Future Medicinal Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 3

Published: March 18, 2025

Language: Английский