3D Printing and Electrospinning of Drug- and Graphene-Enhanced Polycaprolactone Scaffolds for Osteochondral Nasal Repair DOI Open Access
Izabella Rajzer, Anna Kurowska, Anna Nikodem

et al.

Materials, Journal Year: 2025, Volume and Issue: 18(8), P. 1826 - 1826

Published: April 16, 2025

A novel bi-layered scaffold, obtained via 3D printing and electrospinning, was designed to improve osteochondral region reconstruction. The upper electrospun membrane will act as a barrier against unwanted tissue infiltration, while the lower 3D-printed layer provide porous structure for ingrowth. Graphene integrated into scaffold its antibacterial properties, drug Osteogenon® (OST) added promote bone regeneration. composite scaffolds were subjected comprehensive physical, thermal, mechanical evaluations. Additionally, their biological functionality assessed by means of NHAC-kn cells. 0.5% graphene addition PCL significantly increased strain at break, enhancing material ductility. GNP also acted an effective nucleating agent, raising crystallization temperatures supporting mineralization. high surface area facilitated rapid apatite formation attracting calcium phosphate ions. This confirmed FTIR, µCT SEM analyses, which highlighted positive impact on mineral deposition. synergistic interaction between nanoplatelets created bioactive environment that enhanced cell adhesion proliferation, promoted superior formation. These findings highlight scaffold’s potential promising biomaterial repair regenerative medicine.

Language: Английский

Four-Dimensional Printing of β-Tricalcium Phosphate-Modified Shape Memory Polymers for Bone Scaffolds in Osteochondral Regeneration DOI Open Access
Izabella Rajzer, Anna Kurowska, Jarosław Janusz

et al.

Materials, Journal Year: 2025, Volume and Issue: 18(2), P. 306 - 306

Published: Jan. 11, 2025

The use of scaffolds for osteochondral tissue regeneration requires an appropriate selection materials and manufacturing techniques that provide the basis supporting both cartilage bone formation. As are designed to replicate a part replaced ensure cell growth differentiation, implantable have meet various biological requirements, e.g., biocompatibility, biodegradability, mechanical properties. Osteoconductive such as tricalcium phosphate ceramics some biodegradable polymers appear be perfect choice. present work evaluates structural, mechanical, thermal, functional properties shape memory terpolymer modified with β-tricalcium (β-TCP). A new approach is using developed 4D printing, particular focus on its applicability in medical implants. In this study, parameters scaffold components were developed. examined via scanning electron microscopy energy dispersive spectroscopy (SEM-EDS), Fourier-transform infrared (FTIR), differential calorimetry (DSC), testing. cytotoxicity result was obtained MTT assay, alkaline phosphatase (ALP) activity measured. structural microstructural investigations confirmed integration β-TCP into filament matrix scaffolds. Thermal stability enhanced delayed depolymerization polymer matrix. studies demonstrated effective recovery. vitro culture revealed significantly increased viability β-TCP-modified after 3 weeks. can tailored applications which partial recovery acceptable,

Language: Английский

Citations

1

3D Printing and Electrospinning of Drug- and Graphene-Enhanced Polycaprolactone Scaffolds for Osteochondral Nasal Repair DOI Open Access
Izabella Rajzer, Anna Kurowska, Anna Nikodem

et al.

Materials, Journal Year: 2025, Volume and Issue: 18(8), P. 1826 - 1826

Published: April 16, 2025

A novel bi-layered scaffold, obtained via 3D printing and electrospinning, was designed to improve osteochondral region reconstruction. The upper electrospun membrane will act as a barrier against unwanted tissue infiltration, while the lower 3D-printed layer provide porous structure for ingrowth. Graphene integrated into scaffold its antibacterial properties, drug Osteogenon® (OST) added promote bone regeneration. composite scaffolds were subjected comprehensive physical, thermal, mechanical evaluations. Additionally, their biological functionality assessed by means of NHAC-kn cells. 0.5% graphene addition PCL significantly increased strain at break, enhancing material ductility. GNP also acted an effective nucleating agent, raising crystallization temperatures supporting mineralization. high surface area facilitated rapid apatite formation attracting calcium phosphate ions. This confirmed FTIR, µCT SEM analyses, which highlighted positive impact on mineral deposition. synergistic interaction between nanoplatelets created bioactive environment that enhanced cell adhesion proliferation, promoted superior formation. These findings highlight scaffold’s potential promising biomaterial repair regenerative medicine.

Language: Английский

Citations

0