A novel strategy for bone defect repair: Stromal cell-derived factor 1α sustained-release acellular fish scale scaffolds combined with injection of bone marrow mesenchymal stem cells promote bone regeneration
Materials Today Bio,
Journal Year:
2025,
Volume and Issue:
unknown, P. 101759 - 101759
Published: April 1, 2025
Patients
with
bone
defects
often
have
weak
cell
vitality
and
differentiation
ability
of
endogenous
marrow
mesenchymal
stem
cells
(BMSCs),
which
makes
regeneration
face
challenges.
At
present,
the
tissue
engineering
strategies
are
mainly
to
build
grafts
by
loading
on
scaffolds
in
vitro.
These
many
difficulties
that
limit
their
clinical
application.
To
this
end,
we
developed
a
new
strategy
for
defect
repair,
namely
chemotactic
cell-free
combined
BMSCs
injection.
We
first
prepared
polydopamine-functionalized
acellular
fish
scale
scaffold
can
continuously
release
stromal
cell-derived
factor
1α
(SDF-1α)
(termed
as
SDF-1α/PAFS)
vivo
at
least
10
days.
The
study
results
showed
not
only
has
excellent
mechanical
properties
good
biocompatibility
but
also
reactive
oxygen
scavenging
activity,
immunomodulation,
angiogenesis,
osteogenesis.
More
importantly,
SDF-1α/PAFS
recruit
postoperatively
injected
into
repair.
constructed
mouse
cranial
model,
experimental
confirmed
combining
injection
effectively
promote
Overall,
provides
promising
better
convenience
operability.
Language: Английский
Polydopamine-functionalized acellular fish scale scaffolds for accelerated bone tissue regeneration
RSC Advances,
Journal Year:
2025,
Volume and Issue:
15(18), P. 13857 - 13873
Published: Jan. 1, 2025
The
complex
microenvironment
changes
in
the
bone
defect
site
are
main
reason
for
its
refractory
treatment,
including
significant
increase
level
of
reactive
oxygen
species
(ROS)
and
inflammatory
dysregulation.
There
is
an
urgent
need
to
develop
some
bioactive
materials
that
can
regulate
promote
regeneration.
This
study
proposed
a
new
strategy
designing
tissue
engineering
scaffolds
based
on
fish
scales
developed
polydopamine-functionalized
acellular
scale
scaffold
(PDA-AFS).
results
showed
PDA-AFS
had
excellent
mechanical
properties,
special
three-dimensional
surface
topography,
biodegradability.
In
vitro
good
biocompatibility
cell
adhesion
ability,
could
effectively
reduce
ROS
levels,
immunomodulatory
activity.
More
importantly,
enhance
osteogenic
differentiation
marrow
mesenchymal
stem
cells
endogenous
regeneration
critical-sized
calvarial
defects.
addition,
transcriptome
analysis
also
provided
clues
possible
mechanism.
Overall,
we
provide
treatment
Language: Английский