Membrane Fusion‐Based Drug Delivery Liposomes Transiently Modify the Material Properties of Synthetic and Biological Membranes

Small, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Abstract Many drug targets are located in intracellular compartments of cells but they often remain inaccessible to standard imaging and therapeutic agents. To aid delivery, carrier nanoparticles have been used overcome the barrier imposed by plasma membrane. The must entrap large amounts cargo, efficiently quickly deliver cargo cytosol or other compartments, be inert; should not induce cellular responses alter cell state course delivery. This study demonstrates that cationic liposomes with high charge density fuse synthetic membranes membrane living cells. Direct fusion delivers cell‐like vesicles within seconds, bypassing slow inefficient internalization‐based pathways. These effects depend on liposome density, concentration, helper lipid. However, fusion‐mediated delivery results incorporation foreign lipids, causing changes material properties these membranes, namely modifications packing fluidity, induction curvature, decrease surface tension, formation (short‐lived) pores. Importantly, transient removal allows recover their prior interaction.

Language: Английский

An Arginine-rich motif of the HIV-1 TAT protein promotes E. coli cellular entry and DNA delivery

Journal of Drug Delivery Science and Technology, Journal Year: 2025, Volume and Issue: unknown, P. 106864 - 106864

Published: March 1, 2025

Language: Английский