Monitoring Electrochemical Dynamics through Single-Molecule Imaging of hBN Surface Emitters in Organic Solvents DOI Creative Commons

Eveline Mayner,

Nathan Ronceray, Martina Lihter

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 25, 2024

Electrochemical techniques conventionally lack spatial resolution and average local information over an entire electrode. While advancements in have been made through scanning probe methods, monitoring dynamics large areas is still challenging, it would be beneficial to able decouple the from electrode itself. In this work, we leverage single molecule microscopy spatiotemporally monitor analyte surface concentrations a wide area using unmodified hexagonal boron nitride (hBN) organic solvents. Through sensing scheme based on redox-active species interactions with fluorescent emitters at of hBN, observe region linear decrease number against increasingly positive potentials applied nearby We find consistent trends reaction kinetics vs overpotentials between potentiostat-reported currents optically read emitter dynamics, showing Tafel slopes greater than 290 mV·decade

Language: Английский

Real-time label-free imaging of living crystallization-driven self-assembly DOI Creative Commons
Yujie Guo, Tianlai Xia, Vivien Walter

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 18, 2025

Abstract Living crystallization-driven self-assembly (CDSA) of semicrystalline block copolymers is a powerful method for the bottom-up construction uniform polymer microstructures with complex hierarchies. Improving our ability to engineer such particles demands better understanding how precisely control process. Here, we apply interferometric scattering (iSCAT) microscopy observe real-time growth individual poly( ε -caprolactone)-based fibers and platelets. This label-free enables us map role key reaction parameters on platelet rate, size, morphology. Furthermore, iSCAT provides contrast mechanism studying multi-annulus platelets formed via sequential addition different unimers, offering insights into spatial distribution compositions within single platelet.

Language: Английский

Citations

0
Interferometric scattering microscopy DOI
Naomi S. Ginsberg, Chia‐Lung Hsieh, Philipp Kukura

et al.

Nature Reviews Methods Primers, Journal Year: 2025, Volume and Issue: 5(1)

Published: April 10, 2025

Citations

0
Two-color interferometric scattering (iSCAT) microscopy reveals structural dynamics in discrete plasmonic molecules DOI Creative Commons

Leslie Velasco,

Aniqa N. Islam,

Koustav Kundu

et al.

Nanoscale, Journal Year: 2024, Volume and Issue: 16(24), P. 11696 - 11704

Published: Jan. 1, 2024

Plasmonic molecules are discrete assemblies of noble metal nanoparticles (NPs) that interest as transducers in optical nanosensors. So far, NPs with diameters ∼40 nm have been the preferred building blocks for plasmonic intended single molecule sensors due to difficulties associated detecting smaller through elastic scattering conventional darkfield microscopy. Here, we apply 405 nm, 445 two-color interferometric (iSCAT) microscopy characterize polyethylene glycol (PEG) tethered dimers 10 and 20 Ag their monomers. Dimers both NP sizes can be discerned from respective monomers changes average iSCAT contrast. In case NPs, dimer formation induces a change sign contrast, providing characteristic signal binding events. 0.4 kDa 3.4 spacers show contrast distributions significantly different averages on wavelength channels. The measured individual PEG-tethered or function time shows fluctuations indicative rich structural dynamics assembled molecules, which provides an additional metric discern paves path new class plasmon rulers.

Language: Английский

Citations

2
Quantum sensing with optically accessible spin defects in van der Waals layered materials DOI Creative Commons
Hong‐Hua Fang, Xiaojie Wang, X. Marie

et al.

Light Science & Applications, Journal Year: 2024, Volume and Issue: 13(1)

Published: Nov. 5, 2024

Abstract Quantum sensing has emerged as a powerful technique to detect and measure physical chemical parameters with exceptional precision. One of the methods is use optically active spin defects within solid-state materials. These act sensors have made significant progress in recent years, particularly realm two-dimensional (2D) defects. In this article, we focus on latest trends quantum that van der Waals (vdW) We discuss benefits combining addressable 2D vdW materials while highlighting challenges opportunities these To make practical applicable, article identifies some areas worth further exploration. include identifying properties suitable for sensing, generating demand control their spatial localization, understanding impact layer thickness interface integrating photonic structures new functionalities higher emission rates. The explores potential applications several fields, such superconductivity, ferromagnetism, nanoelectronics, biology. For instance, nanoscale microfluidic technology nanopore may lead platform DNA sequencing. As continues evolve, advancement defect engineering techniques, are expected play vital role sensing.

Language: Английский

Citations

2
Surface passivation and functionalisation for mass photometry DOI Creative Commons
Jenny Sülzle, Laila Elfeky, Suliana Manley

et al.

Journal of Microscopy, Journal Year: 2024, Volume and Issue: 295(1), P. 14 - 20

Published: April 12, 2024

Abstract Interferometric scattering (iSCAT) microscopy enables the label‐free observation of biomolecules. Consequently, single‐particle imaging and tracking with iSCAT‐based method known as mass photometry (MP) is a growing area study. However, establishing reliable cover glass passivation functionalisation methods crucial to reduce nonspecific binding prepare surfaces for in vitro single‐molecule experiments. Existing protocols fluorescence can contain strongly or mobile components, which make them impractical MP‐based microscopy. In this study, we characterise several different surface coatings using MP. We present approaches 3‐aminopropyltriethoxysilane (APTES) polyethylene glycol (PEG, 2k) along via maleimide‐thiol linker. These are compatible water salt buffers, show low background scattering; thus, able measure proteins small 60 kDa. technical note, offer preparation suitable experiments

Language: Английский

Citations

1
Site-Specific Integration of Hexagonal Boron Nitride Quantum Emitters on 2D DNA Origami Nanopores DOI Creative Commons
Yabin Wang, Ze Yu, Carlas Smith

et al.

Nano Letters, Journal Year: 2024, Volume and Issue: 24(28), P. 8510 - 8517

Published: June 10, 2024

Optical emitters in hexagonal boron nitride (hBN) are promising probes for single-molecule sensing platforms. When engineered nanoparticle form, they can be integrated as detectors nanodevices, yet positional control at the nanoscale is lacking. Here we demonstrate functionalization of DNA origami nanopores with optically active hBN nanoparticles (NPs) nanometer precision. The NPs under three wavelengths visible illumination and display both stable blinking emission, enabling their accurate localization by using wide-field optical nanoscopy. Correlative opto-structural characterization reveals deterministic binding bright, multicolor pore rim due to π–π stacking interactions site-specific locations on origami. Our work provides a scalable, bottom-up approach toward assembly solid-state arbitrary structural elements based Such arrangement components advance development platforms, including nanochannel sensors.

Language: Английский

Citations

1
Diffusion of DNA on Atomically Flat 2D Material Surfaces DOI Creative Commons
Chirlmin Joo, Dong Hoon Shin, Sung Hyun Kim

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 12, 2024

Abstract Accurate localization and delivery of biomolecules is pivotal for building tools to understand biology. The interactions with atomically flat 2D surfaces offer a means realize both the delivery, yet experimental utilization such has remained elusive. By combining single-molecule detection methods computational approaches, we have comprehensively characterized individual DNA molecules hexagonal boron nitride (hBN) surfaces. Our experiments directly show that, upon binding hBN surface, molecule retains its ability diffuse along surface. Further, that magnitude direction diffusion can be controlled by length, surface topography, atomic defects. fabricating narrow ribbon structure, achieved pseudo-1D confinement, demonstrating potential nanofluidic guiding biomolecules. work sets stage engineering materials high-throughput manipulation single their applications in nanobiotechnology.

Language: Английский

Citations

0
Surface passivation and functionalization for interferometric scattering microscopy DOI Open Access
Jenny Sülzle, Laila Elfeky, Suliana Manley

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 1, 2024

Abstract Interferometric scattering (iSCAT) microscopy enables the label-free observation of biomolecule interactions with surfaces. Consequently, single-particle imaging and tracking iSCAT is a growing area study. However, establishing reliable cover glass passivation functionalization methods are crucial to reduce non-specific binding prepare surfaces for in vitro single-molecule experiments. Existing protocols fluorescence can contain strongly or mobile components, which make them impractical iSCAT-based microscopy. In this study, we characterize several different surface coatings using iSCAT. We present approaches 3-aminopropyltriethoxysilane (APTES) polyethylene glycol (PEG, 2k) along via maleimide-thiol linker. These compatible water salt buffers, show low background scattering; thus, able measure proteins as small 60 kDa. technical note, offer preparation suitable experiments

Language: Английский

Citations

0
Monitoring Electrochemical Dynamics through Single-Molecule Imaging of hBN Surface Emitters in Organic Solvents DOI Creative Commons

Eveline Mayner,

Nathan Ronceray, Martina Lihter

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 25, 2024

Electrochemical techniques conventionally lack spatial resolution and average local information over an entire electrode. While advancements in have been made through scanning probe methods, monitoring dynamics large areas is still challenging, it would be beneficial to able decouple the from electrode itself. In this work, we leverage single molecule microscopy spatiotemporally monitor analyte surface concentrations a wide area using unmodified hexagonal boron nitride (hBN) organic solvents. Through sensing scheme based on redox-active species interactions with fluorescent emitters at of hBN, observe region linear decrease number against increasingly positive potentials applied nearby We find consistent trends reaction kinetics vs overpotentials between potentiostat-reported currents optically read emitter dynamics, showing Tafel slopes greater than 290 mV·decade

Language: Английский

Citations

0