Boosting High‐Fidelity Isotropic Super‐Resolution via Image Interference Structured Illumination Microscopy with Spatial‐Spectral Optimization DOI

Enxing He,

Yile Sun, Hongfei Zhu

et al.

Laser & Photonics Review, Journal Year: 2025, Volume and Issue: unknown

Published: April 30, 2025

Abstract Spatial resolution is crucial for imaging subcellular structures. The advent of three‐dimensional structured illumination microscopy (3D‐SIM) greatly benefits the biology community, providing a powerful tool organelles with twofold enhancement in all three dimensions. However, axial 3D‐SIM limited to around 300 nm, which inferior its lateral resolution. Here, novel method called image interference SIM () reported, utilizes two oppositely positioned objectives detect fluorescence emission under three‐beam excitation. By incorporating spectral modulation and spatial domain Frobenius‐Hessian optimization, achieves an approximately twice that 3D‐SIM, reaching 130 nm. Furthermore, potential structures demonstrated on various biological samples, including microtubules, actin filaments, mitochondrial outer membranes. enhanced optical sectioning capability can be utilized resolve are challenging discern using ordinary 3D‐SIM.

Language: Английский

Boosting High‐Fidelity Isotropic Super‐Resolution via Image Interference Structured Illumination Microscopy with Spatial‐Spectral Optimization DOI

Enxing He,

Yile Sun, Hongfei Zhu

et al.

Laser & Photonics Review, Journal Year: 2025, Volume and Issue: unknown

Published: April 30, 2025

Abstract Spatial resolution is crucial for imaging subcellular structures. The advent of three‐dimensional structured illumination microscopy (3D‐SIM) greatly benefits the biology community, providing a powerful tool organelles with twofold enhancement in all three dimensions. However, axial 3D‐SIM limited to around 300 nm, which inferior its lateral resolution. Here, novel method called image interference SIM () reported, utilizes two oppositely positioned objectives detect fluorescence emission under three‐beam excitation. By incorporating spectral modulation and spatial domain Frobenius‐Hessian optimization, achieves an approximately twice that 3D‐SIM, reaching 130 nm. Furthermore, potential structures demonstrated on various biological samples, including microtubules, actin filaments, mitochondrial outer membranes. enhanced optical sectioning capability can be utilized resolve are challenging discern using ordinary 3D‐SIM.

Language: Английский

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