Next Generation Non-Invasive Biomarker Testing in Saliva: The Organic Electrolyte Gated Field Effect Transistor Aptasensor System DOI Open Access
Roslyn S. Massey

Published: Jan. 1, 2023

Diagnostic biomolecule quantification in healthcare relies on effective but centralized laboratory testing procedures. However, as the need for rapidly grows, current process is struggling to meet demand. Rapid point-of-contact analysis devices are not capable of non-invasive small, dilute molecules such steroid hormones saliva. Current literature bioquantification suffer from drawbacks short shelf life, high intra-device variability, sample matrix effects, material incompatibilities, high-cost fabrication and data collection that rely conditions processes. This body work took our organic electrolyte gated field effect transistor (OEGFET), a novel biosensor architecture, developed it into feasible platform real-world applications. Iterative development through architecture prototyping, optimization selection has solved key challenges scale biosensors whilst adding functionalities. Expensive, specialized requirements gradually replaced with wet lab-based solution processing techniques facile manufacturing, making cost effective, flexible, suitable scale-up manufacturing. The OEGFET improved electrical characteristics: on/off ratio (from 140 up 103); reduced leakage currents 90nA down 5.8nA); threshold voltage 4V 0.23V). Real world needs biosensing applications life 3 days 1 month), translation dopamine cortisol α-synuclein been demonstrated selectivity broad, physiologically relevant detection range (over 1mM–100fM, limit 10fM) buffer solutions complex samples spiked saliva supernatant. Key additional functionalities integration bespoke small footprint printed circuit board low power (<300mW) thus removing reliance equipment, integrated microfluidic channels consistent plate separation handling. resulting handling fluidics, low-power operation, simple method, broadly applicable. Presently, neurotransmitters, proteins, hormones. During course author’s doctoral program was transformed potential rapid, quantification.

Language: Английский

Trends in Aptasensing and the Enhancement of Diagnostic Efficiency and Accuracy DOI
Mohammad A. Ansari, Damini Verma,

Mohd-Akmal Hamizan

et al.

ACS Synthetic Biology, Journal Year: 2025, Volume and Issue: 14(1), P. 21 - 40

Published: Jan. 6, 2025

The field of healthcare diagnostics is navigating complex challenges driven by evolving patient demographics and the rapid advancement new technologies worldwide. In response to these challenges, biosensors offer distinctive advantages over traditional diagnostic methods, such as cost-effectiveness, enhanced specificity, adaptability, making their integration with point-of-care (POC) platforms more feasible. recent years, aptasensors have significantly evolved in capabilities through emerging microfluidics, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) systems, wearable devices, machine learning (ML), driving progress precision medicine global solutions. Moreover, advancements not only improve accuracy but also hold potential revolutionize early detection, reduce costs, outcomes, especially resource-limited settings. This Account examines key advancements, focusing on how scientific breakthroughs, including artificial intelligence (AI), improved sensitivity precision. Additionally, has enabled real-time monitoring data analysis, fostering advances personalized healthcare. Furthermore, commercialization aptasensor could increase availability clinical settings support use widespread solutions for health challenges. Hence, this review discusses technological improvements, practical uses, prospects while surrounding standardization, validation, interdisciplinary collaboration application. Finally, ongoing efforts address are ensure that can be effectively implemented diverse systems.

Language: Английский

Citations

2
Aptamer selection via versatile microfluidic platforms and their diverse applications DOI

Yi-Da Chung,

Yi-Cheng Tsai,

C.W. Wang

et al.

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

This review examines contributions of microfluidic technology to SELEX-based aptamer identification, with alternative methods such as in vivo -like SELEX and Non-SELEX for selecting aptamers discussed critical steps over the past decade.

Language: Английский

Citations

2
Pain-Free Alpha-Synuclein Detection by Low-Cost Hierarchical Nanowire Based Electrode DOI Creative Commons
Gisella Maria Di Mari, M. Scuderi, Giuseppe Lanza

et al.

Nanomaterials, Journal Year: 2024, Volume and Issue: 14(2), P. 170 - 170

Published: Jan. 12, 2024

Analytical methods for the early detection of neurodegenerative biomarker Parkinson’s disease (PD), α-synuclein, are time-consuming and invasive, require skilled personnel sophisticated expensive equipment. Thus, a pain-free, prompt simple α-synuclein biosensor in plasma is highly demanded. In this paper, an electrochemical based on hierarchical polyglutamic acid/ZnO nanowires decorated by gold nanoparticles, assembled as nanostars (NSs), determination human proposed. ZnO NSs were prepared chemical bath deposition (CBD) with electrodeposited Au nanoparticles (Au NPs). Then, electro-polymerized glutamic acid was grown functionalized anti-α-synuclein. A synergistic enhancement electrode sensitivity observed when NPs embedded into NSs. The analytical performance evaluated cyclic voltammetry (CV) impedance spectroscopy (EIS), using Fe(II)(CN)64−/Fe(III)(CN)63− probe. charge transfer resistance after recognition found to be linear, concentration range 0.5 pg·mL−1 10 pg·mL−1, limit 0.08 good reproducibility (5% variation) stability (90%). also shown reliably discriminate between healthy PD plasma. These results suggest that proposed provides rapid, quantitative high-sensitivity result content plasma, represents feasible tool capable accelerating non-invasive identification disease.

Language: Английский

Citations

13
Trends in ready-to-use portable electrochemical sensing devices for healthcare diagnosis DOI

Anjana Sajeevan,

Reshmi A. Sukumaran,

Lakshmi R. Panicker

et al.

Microchimica Acta, Journal Year: 2025, Volume and Issue: 192(2)

Published: Jan. 14, 2025

Language: Английский

Citations

1
A Surface Imprinted Polymer EIS Sensor for Detecting Alpha-Synuclein, a Parkinson’s Disease Biomarker DOI Creative Commons
Roslyn S. Massey,

Rishabh Ramesh Appadurai,

Ravi Prakash

et al.

Micromachines, Journal Year: 2024, Volume and Issue: 15(2), P. 273 - 273

Published: Feb. 15, 2024

Parkinson’s Disease (PD) is a debilitating neurodegenerative disease, causing loss of motor function and, in some instances, cognitive decline and dementia those affected. The quality life can be improved, disease progression delayed through early interventions. However, current methods confirming PD diagnosis are extremely invasive. This prevents their use as screening tool for the onset stages PD. We propose surface imprinted polymer (SIP) electroimpedance spectroscopy (EIS) biosensor detecting α-Synuclein (αSyn) its aggregates, biomarker that appears saliva blood during blood-brain barrier degrades. stamp fabricated by low-temperature melt stamping polycaprolactone (PCL) on interdigitated EIS electrodes. result low-cost, small-footprint highly suitable non-invasive monitoring biomarker. sensors were tested with αSyn dilutions deionized water constant ionic concentration matrix solutions decreasing concentrations to remove background effects concentration. device response confirmed specificity these devices target protein monomeric αSyn. sensor limit detection was measured 5 pg/L, linear range pg/L–5 µg/L. covers physiological makes this promising method quantifying monomers patients future. SIP regenerated, reused demonstrate capability repeat sensing potential continuous

Language: Английский

Citations

5
High pH stability and detection of α-synuclein using an EGFET biosensor with an HfO2 gate deposited by high-power pulsed magnetron sputtering DOI
Chia‐Ming Yang,

Chaohui Wei,

Fuad Ughi

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 416, P. 136006 - 136006

Published: May 28, 2024

Language: Английский

Citations

5
Recent Advances in Electrochemical Biosensors for Neurodegenerative Disease Biomarkers DOI Creative Commons

Minkun Bae,

Nayoung Kim, Euni Cho

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(3), P. 151 - 151

Published: Feb. 28, 2025

Neurodegenerative diseases, such as Parkinson’s disease (PD) and Alzheimer’s (AD), represent a growing global health challenge with overlapping biomarkers. Key biomarkers, including α-synucleins, amyloid-β, Tau proteins, are critical for accurate detection but often assessed using conventional methods like enzyme-linked immunosorbent assay (ELISA) polymerase chain reaction (PCR), which invasive, costly, time-intensive. Electrochemical biosensors have emerged promising tools biomarker due to their high sensitivity, rapid response, potential miniaturization. The integration of nanomaterials has further enhanced performance, improving specificity, practical application. To this end, review provides comprehensive overview recent advances in electrochemical detecting neurodegenerative highlighting strengths, limitations, future opportunities. By addressing the challenges early diagnosis, work aims stimulate interdisciplinary innovation improve clinical outcomes patients.

Language: Английский

Citations

0
Stamp-Imprinted Polymer EIS Biosensor for Amyloid-Beta Detection: A Novel Approach Towards Alzheimer’s Screening DOI Creative Commons

Chloé E. D. Davidson,

Ravi Prakash

Biosensors, Journal Year: 2025, Volume and Issue: 15(4), P. 228 - 228

Published: April 3, 2025

Surface-imprinted polymers (SIPs) represent an exciting and cost-effective alternative to antibodies for electrochemical impedance spectroscopy (EIS)-based biosensing. They can be produced using simple printing techniques have shown high efficacy in detecting large biomolecules microorganisms. Stamp imprinting, a novel SIP method, creates the target analyte’s imprint soft lithography mask of analyte matrix, thereby reducing material complexities eliminating need cross-linking, which makes process more scalable than conventional SIPs. In this work, we demonstrate stamp-imprinted EIS biosensor biocompatible polymer, polycaprolactone (PCL), quantifying amyloid beta-42 (Aβ-42), small peptide involved pathophysiology Alzheimer’s disease. The evaluated SIP-EIS biosensors showed detection limit close 10 fg/mL, range covering physiologically relevant concentration blood serum (from fg/mL μg/mL). device sensitivity, is found comparable antibody-based devices, demonstrates potential as diagnostic approaches. We also evaluate viability analyzing these proteins complex media, notably presence albumin proteins, cause biofouling non-specific interactions. combination selectivity, ease fabrication particularly suited portable point-of-care applications.

Language: Английский

Citations

0
Biosensors for Early Detection of Parkinson’s Disease: Principles, Applications, and Future Prospects DOI Creative Commons
Panpan Jiang, Nan Gao, Gang Chang

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(5), P. 280 - 280

Published: April 29, 2025

Parkinson’s disease (PD), a neurodegenerative disorder marked by the progressive loss of dopaminergic neurons in substantia nigra, imposes substantial economic burdens, including both direct and indirect costs. The medical community currently lacks definitive cure for disease, early detection is crucial timely intervention management. As innovative diagnostic tools, biosensors have shown great potential detecting PD at its stages. This review comprehensively summarizes recent advances PD, with particular focus on two key biomarkers: dopamine (DA) α-synuclein (α-syn). Furthermore, it illustrates variety nanotechnology-based biosensors, optical, electrochemical, transistor detailing their underlying principles, advantages, limitations, applications detection. Moreover, explores challenges prospects advancing diagnosis.

Language: Английский

Citations

0
Biosensors based on organic transistors for intraoral biomarker detection DOI

R. Mai,

Yongsheng Zhou, Kangning Zhao

et al.

Microchimica Acta, Journal Year: 2025, Volume and Issue: 192(6)

Published: May 5, 2025

Language: Английский

Citations

0