Next Generation Non-Invasive Biomarker Testing in Saliva: The Organic Electrolyte Gated Field Effect Transistor Aptasensor System DOI Open Access
Roslyn S. Massey

Published: Jan. 1, 2023

Diagnostic biomolecule quantification in healthcare relies on effective but centralized laboratory testing procedures. However, as the need for rapidly grows, current process is struggling to meet demand. Rapid point-of-contact analysis devices are not capable of non-invasive small, dilute molecules such steroid hormones saliva. Current literature bioquantification suffer from drawbacks short shelf life, high intra-device variability, sample matrix effects, material incompatibilities, high-cost fabrication and data collection that rely conditions processes. This body work took our organic electrolyte gated field effect transistor (OEGFET), a novel biosensor architecture, developed it into feasible platform real-world applications. Iterative development through architecture prototyping, optimization selection has solved key challenges scale biosensors whilst adding functionalities. Expensive, specialized requirements gradually replaced with wet lab-based solution processing techniques facile manufacturing, making cost effective, flexible, suitable scale-up manufacturing. The OEGFET improved electrical characteristics: on/off ratio (from 140 up 103); reduced leakage currents 90nA down 5.8nA); threshold voltage 4V 0.23V). Real world needs biosensing applications life 3 days 1 month), translation dopamine cortisol α-synuclein been demonstrated selectivity broad, physiologically relevant detection range (over 1mM–100fM, limit 10fM) buffer solutions complex samples spiked saliva supernatant. Key additional functionalities integration bespoke small footprint printed circuit board low power (<300mW) thus removing reliance equipment, integrated microfluidic channels consistent plate separation handling. resulting handling fluidics, low-power operation, simple method, broadly applicable. Presently, neurotransmitters, proteins, hormones. During course author’s doctoral program was transformed potential rapid, quantification.

Language: Английский

Biosensors for Early Detection of Parkinson’s Disease: Principles, Applications, and Future Prospects DOI Creative Commons
Panpan Jiang, Nan Gao, Gang Chang

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(5), P. 280 - 280

Published: April 29, 2025

Parkinson’s disease (PD), a neurodegenerative disorder marked by the progressive loss of dopaminergic neurons in substantia nigra, imposes substantial economic burdens, including both direct and indirect costs. The medical community currently lacks definitive cure for disease, early detection is crucial timely intervention management. As innovative diagnostic tools, biosensors have shown great potential detecting PD at its stages. This review comprehensively summarizes recent advances PD, with particular focus on two key biomarkers: dopamine (DA) α-synuclein (α-syn). Furthermore, it illustrates variety nanotechnology-based biosensors, optical, electrochemical, transistor detailing their underlying principles, advantages, limitations, applications detection. Moreover, explores challenges prospects advancing diagnosis.

Language: Английский

Citations

0
Is this the Simplest Possible Biosensor? A Surface Imprinted Polymer EIS Sensor for Detecting Alpha-synuclein, a Parkinson’s Disease Biomarker DOI Open Access
Roslyn S. Massey,

Rishabh Ramesh Appadurai,

Ravi Prakash

et al.

Published: Jan. 25, 2024

Parkinson&rsquo;s Disease (PD) is a debilitating neurodegenerative disease, causing loss of motor function, and in some instances, cognitive decline dementia, those affected. The quality life can be improved, disease progression delayed through early interventions, however, current methods confirming PD diagnosis are extremely invasive. This prevents their use as screening tool for the onset stages PD. We propose surface imprinted polymer (SIP) electroimpedance spectroscopy (EIS) biosensor detecting &alpha;-Synuclein (&alpha;Syn) its aggregates, biomarker that appears saliva blood during brain barrier degrades. stamp fabricated by low temperature melt stamping polycaprolactone (PCL) on interdigitated EIS electrodes. result low-cost, small footprint highly suitable non-invasive monitoring biomarker. sensors were tested with &alpha;Syn dilutions deionized water, constant ionic concentration matrix solutions decreasing concentrations to remove background effects concentration. device response confirmed specificity these devices target protein monomeric &alpha;Syn. sensor limit detection was measured 5 pg/L linear range &ndash; &micro;g/L. covers physiological makes this promising method quantifying monomers patients future. SIP regenerated, reused demonstrate capability repeat sensing potential continuous

Language: Английский

Citations

2
Selection of DNA aptamers that prevent the fibrillization of α-synuclein protein in cellular and mouse models DOI Creative Commons
Erin M. McConnell, Dennis Chan,

Katelyn Ventura

et al.

Molecular Therapy — Nucleic Acids, Journal Year: 2024, Volume and Issue: 35(3), P. 102251 - 102251

Published: June 15, 2024

Language: Английский

Citations

2
Biosensors for Parkinson’s Disease: Where Are We Now, and Where Do We Need to Go? DOI

Grace M. Maddocks,

Miriam Eisenstein, H. Tom Soh

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(9), P. 4307 - 4327

Published: Aug. 27, 2024

Parkinson's Disease is the second most common neurological disease in United States, yet there no cure, pinpointed cause, and definitive diagnostic procedure. typically diagnosed when patients present with motor symptoms such as slowness of movement tremors. However, none these are specific to Parkinson's, a confident diagnosis only achieved 60-80% dopaminergic neurons longer functioning, at which point much damage brain irreversible. This Perspective details ongoing efforts accomplishments biosensor research goal overcoming issues for care, focus on potential impact early associated opportunities pinpoint cause cure. We critically analyze strengths shortcomings current technologies discuss ideal characteristics technology toolbox guide future decisions this space. Finally, we assess what role biosensors can play facilitating precision medicine patients.

Language: Английский

Citations

2
Biosensing systems for the detection of biomarkers of neurodegenerative diseases: a review DOI
Z Zielińska, Łukasz Ołdak, Ewa Gorodkiewicz

et al.

Talanta, Journal Year: 2024, Volume and Issue: 284, P. 127247 - 127247

Published: Nov. 19, 2024

Language: Английский

Citations

1
Saliva Analysis Based on Microfluidics: Focusing the Wide Spectrum of Target Analyte DOI
Xinjun Liu, Xinyue Zhou, Xiaojia Li

et al.

Critical Reviews in Analytical Chemistry, Journal Year: 2023, Volume and Issue: unknown, P. 1 - 23

Published: Dec. 1, 2023

AbstractSaliva is one of the most critical human body fluids that can reflect state body. The detection saliva great significance for disease diagnosis and health monitoring. Microfluidics, characterized by microscale size high integration, an ideal platform development rapid low-cost diagnostic techniques devices. Microfluidic-based testing methods have aroused considerable interest due to increasing need noninvasive frequent or long-term testing. This review briefly described analysis generally classified targets in into pathogenic microorganisms, inorganic substances, organic substances. By using this classification as a benchmark, state-of-the-art research results on microfluidic various substances were summarized. work also put forward challenges future directions saliva.Keywords: Analysismicrofluidicssaliva Disclosure statementThe authors declare they no known competing financial interests personal relationships could appeared influence reported paper.Data availability statementNo data was used article.Author contributionsX.L., XT.S. HZ.Y. conceptualized idea review. X.L. wrote initial drafts manuscript. XY.Z., XJ.L., YX.W. TL.W. contributed collection graph making. S.L. writing manuscript improvement. All reviewing gave approval final version manuscript.Additional informationFundingThis funded project from China Post-doctoral Science Foundation (No. 2021MD703911), Natural Liaoning Province 2020-MS-166), Education Department QN2019035), National 81500897).

Language: Английский

Citations

1
Electrolyte-gated FET Biosensors DOI
Benoı̂t Piro

Royal Society of Chemistry eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 28 - 72

Published: May 3, 2024

After a general introduction, the opening section of this chapter presents broad introduction to electrolyte-gated field-effect transistors (EGFETs). This encompasses fundamental concepts surrounding electrical double layers, charge transport in conductive and semiconductive materials, polarization, interfacial capacitance, underlying operating principles. The following examines active including organic inorganic semiconductors, nanostructured conductors such as nanowires, carbon nanotubes graphene. Furthermore, explores techniques utilized produce EGFETs, vacuum thermal deposition inkjet printing. fourth fabrication methods, while fifth reviews common biological probes DNA aptamers, antibodies, nanobodies, proteins, enzymes or synthetic ligands. Additionally, it discusses their application EGFETs anticipated transduction mechanisms. concluding provides various exemplary instances extracted from relevant literature. does not include discussion electrochemical (OECTs) ion-sensitive (ISFETs).

Language: Английский

Citations

0
ß-amyloid as a Novel Target Biomarker for the OEGFET Biosensor, Revolutionizing Non-Invasive Alzheimer's Screening DOI
S. Johri, Roslyn S. Massey, Dennis Chan

et al.

IEEE Sensors Letters, Journal Year: 2024, Volume and Issue: 8(6), P. 1 - 4

Published: May 20, 2024

Early detection of neurodegenerative diseases has the potential to slow disease progression by timely interventions and effective management. Alzheimer's Disease (AD), most common form dementia globally, a preclinical phase that lasts decades prior prodromal stage. Preclinical stage neurological changes are accompanied in biomarker concentrations such as Amyloid Beta peptides (Aß), however, at present there no cost-effective non-invasive quantification methods suitable for population screening. To meet this need, we have tailored our proven Organic Electrolyte Gated Field Effect Transistor (OEGFET) biosensor Aß serum saliva samples. This was achieved incorporating covalently bound antibody (AB) within soft-fluidic microchannel biosensor, significant advancement demonstrates robustness sensor system towards different types bioreceptors target biomarkers. Furthermore, AB-OEGFET created using flexible substrates polymers due their impressive biocompatibility. We observed characteristic OEGFET device current concentration correlation behavior all tested media, including specimens. The response buffer recorded from 10μg/ml 100fg/ml, limit (LOD) range 100 ng/ml spiking tests specificity investigated samples spiked with non-binding protein analyte, α-synuclein. predictable targeting solutions crowded numerous other proteomic background ionic higher promote some non-specific binding bioreceptor adding competing matrix effect. Irrespective these exceptions, first demonstration integration receptors electronics, explored study, holds substantial promise disposable, biosensors AD.

Language: Английский

Citations

0
Comparison of Aptamer and Antibody Bioreceptors in the OEGFET Biosensor Platform for Detecting α-Synuclein, a Parkinson's Biomarker DOI
Roslyn S. Massey, S. Johri, Dennis Chan

et al.

IEEE Sensors Letters, Journal Year: 2024, Volume and Issue: 8(7), P. 1 - 4

Published: June 12, 2024

Language: Английский

Citations

0
High‐Performance Flexible and Printed Graphene‐Field Effect Biosensor for Ferritin Detection DOI Open Access
Kamalesh Tripathy, Mitradip Bhattacharjee

Advanced Materials Technologies, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 27, 2024

Abstract A flexible and printed field‐effect biosensor with a graphene channel (Gr‐FET biosensor) is designed characterized to detect ferritin. The offers an onboard Ag/AgCl gate give stable transfer characteristics significantly low leakage current of 0.06% the minimum drain current. Further, proposed Gr‐FET shows 1.5–3 times higher transconductance ( g m ) value m,hole up 400 µS m,electron 250 µS, compared reported electrolyte‐gated on substrates. operation also optimized have minimal hysteresis effect for reliable sensing operation. fairly linear at ferritin concentration (0.05–0.5 µg L −1 sensitivity as high ≈230 mV/(µg very limit detection (LOD) ≈27 ng . Given cost‐effective fabrication process scalability, can be deployed large scale early diagnosis iron deficiency anemia.

Language: Английский

Citations

0