Published: Jan. 1, 2023

Diagnostic biomolecule quantification in healthcare relies on effective but centralized laboratory testing procedures. However, as the need for rapidly grows, current process is struggling to meet demand. Rapid point-of-contact analysis devices are not capable of non-invasive small, dilute molecules such steroid hormones saliva. Current literature bioquantification suffer from drawbacks short shelf life, high intra-device variability, sample matrix effects, material incompatibilities, high-cost fabrication and data collection that rely conditions processes. This body work took our organic electrolyte gated field effect transistor (OEGFET), a novel biosensor architecture, developed it into feasible platform real-world applications. Iterative development through architecture prototyping, optimization selection has solved key challenges scale biosensors whilst adding functionalities. Expensive, specialized requirements gradually replaced with wet lab-based solution processing techniques facile manufacturing, making cost effective, flexible, suitable scale-up manufacturing. The OEGFET improved electrical characteristics: on/off ratio (from 140 up 103); reduced leakage currents 90nA down 5.8nA); threshold voltage 4V 0.23V). Real world needs biosensing applications life 3 days 1 month), translation dopamine cortisol α-synuclein been demonstrated selectivity broad, physiologically relevant detection range (over 1mM–100fM, limit 10fM) buffer solutions complex samples spiked saliva supernatant. Key additional functionalities integration bespoke small footprint printed circuit board low power (<300mW) thus removing reliance equipment, integrated microfluidic channels consistent plate separation handling. resulting handling fluidics, low-power operation, simple method, broadly applicable. Presently, neurotransmitters, proteins, hormones. During course author’s doctoral program was transformed potential rapid, quantification.

Language: Английский