Angewandte Chemie,
Journal Year:
2024,
Volume and Issue:
136(29)
Published: May 14, 2024
Abstract
Electrochemiluminescence
(ECL)
is
rapidly
evolving
from
an
analytical
method
into
optical
microscopy.
The
orthogonality
of
the
electrochemical
trigger
and
readout
distinguishes
it
classic
microscopy
techniques,
owing
to
its
near‐zero
background,
remarkable
sensitivity,
absence
photobleaching
phototoxicity.
In
this
minireview,
we
summarize
recent
advances
in
ECL
imaging
technology,
emphasizing
original
configurations
which
enable
biological
entities
improvement
properties
by
increasing
complexity
multiplexing
bioassays.
Additionally,
mapping
(electro)chemical
reactivity
space
provides
valuable
information
on
nanomaterials
facilitates
deciphering
mechanisms
for
improving
their
performances
diagnostics
(electro)catalysis.
Finally,
highlight
achievements
at
ultimate
limits
single
molecules,
photons
or
chemical
reactions,
current
challenges
translate
other
fields
such
as
material
science,
catalysis
biology.
Angewandte Chemie International Edition,
Journal Year:
2024,
Volume and Issue:
63(29)
Published: May 14, 2024
Electrochemiluminescence
(ECL)
is
rapidly
evolving
from
an
analytical
method
into
optical
microscopy.
The
orthogonality
of
the
electrochemical
trigger
and
readout
distinguishes
it
classic
microscopy
techniques,
owing
to
its
near-zero
background,
remarkable
sensitivity,
absence
photobleaching
phototoxicity.
In
this
minireview,
we
summarize
recent
advances
in
ECL
imaging
technology,
emphasizing
original
configurations
which
enable
biological
entities
improvement
properties
by
increasing
complexity
multiplexing
bioassays.
Additionally,
mapping
(electro)chemical
reactivity
space
provides
valuable
information
on
nanomaterials
facilitates
deciphering
mechanisms
for
improving
their
performances
diagnostics
(electro)catalysis.
Finally,
highlight
achievements
at
ultimate
limits
single
molecules,
photons
or
chemical
reactions,
current
challenges
translate
other
fields
such
as
material
science,
catalysis
biology.
Journal of the American Chemical Society,
Journal Year:
2024,
Volume and Issue:
146(15), P. 10299 - 10311
Published: April 9, 2024
The
chemical
industry
is
a
major
consumer
of
fossil
fuels.
Several
reactions
practical
value
proceed
with
the
gain
or
loss
electrons,
opening
path
to
integrate
renewable
electricity
into
manufacturing.
However,
most
organic
molecules
have
low
aqueous
solubility,
causing
green
and
cheap
electricity-driven
suffer
from
intrinsically
reaction
rates
in
industry's
solvent
choice:
water.
Here,
we
show
that
strategic,
partial
electrode
fouling
hydrophobic
insulators
(oils
plastics)
offsets
kinetic
limitations
caused
by
poor
reactant
new
for
direct
integration
production
commodity
chemicals.
Through
electrochemiluminescence
microscopy,
reveal
oxidation
reactants
up
6-fold
rate
increase
at
"fouled"
oil–electrolyte–electrode
interface
relative
clean
electrolyte–electrode
areas.
Analogously,
electrodes
partially
masked
(fouled)
plastic
patterns,
deposited
either
photolithographically
(photoresists)
manually
(inexpensive
household
glues
sealants),
outperform
electrodes.
effect
not
limited
water
and,
example,
net
gold
electrodeposition
are
22%
larger
fouled
than
In
system
involving
surface-active
reactant,
augmentation
driven
synergy
between
insulator-confined
enrichment
insulator-induced
current
crowding,
whereas
only
latter
possibly
localized
decrease
iR
drop
near
insulator
relevant
composed
non-surface-active
species.
Our
counterintuitive
design
enhances
electrolysis
despite
diminished
area
intimate
contact
introduces
upscaling
electrochemical
processes.
Sensors & Diagnostics,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Jan. 1, 2024
Herein,
we
review
the
recent
developments
and
applications
of
ECL
for
immunosensing
in
different
areas,
such
as
detection
biomarkers,
bead-based
bacteria
cell
analysis,
provide
future
perspectives
new
developments.
Advanced Functional Materials,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 16, 2024
Abstract
The
unmet
on‐site
clinical
diagnostics
are
increasingly
demanding
portable
bioassays,
but
compromised
by
sensitivity
and
throughput.
Herein,
a
nanostructured
bubbles‐enhanced
fluorescence
(nBEF)
assay
is
reported
interfacing
with
smartphone
imaging
system
to
enable
portable,
ultrasensitive,
specific
miRNA
detection
in
parallel.
In
nBEF,
the
high‐curvature
bubbles
allow
physically
enhancing
optical
propagation,
chemically
fluorescence,
assembling
more
DNA
probes,
undergoing
faster
self‐suspension
efficient
self‐aggregation
enhancement
relative
their
smooth
counterparts.
By
coupling
bubble‐driven
signal
amplification
plug‐and‐play
concatemer,
system,
this
nBEF
achieves
ultrasensitive
fluorescent
of
at
femtomolar
level
one‐base
resolution
single
test,
capable
monitoring
drug‐induced
dynamic
expression
accurate
cancer
diagnosis
(AUC
=
1)
through
profiling
40
plasma
samples
after
RNA
extraction.
This
further
integrates
an
R‐value
analysis
RGB
module
realize
automated,
readout.
Such
high‐performance
provides
low‐cost,
highly
sensitive
tool
diagnosis.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(45), P. 18262 - 18268
Published: Nov. 1, 2024
Optimization
of
electrochemiluminescence
(ECL)
immunoassays
is
highly
beneficial
for
enhancing
clinical
diagnostics.
A
major
challenge
the
improvement
operation
conditions
required
bead-based
using
typical
[Ru(bpy)3]2+/tri-n-propylamine
(TPrA)
system.
In
this
study,
we
report
a
heterogeneous
immunoassay
based
on
near-infrared
photoinduced
ECL,
which
facilitates
imaging
and
quantitative
analysis
[Ru(bpy)3]2+-modified
immunobeads
at
low
anodic
potential.
The
photovoltage
generated
by
photoanode
under
light
promotes
oxidation
processes
electrode/electrolyte
interface,
thus
considerably
lowering
onset
potential
both
TPrA
ECL
emission.
anti-Stokes
shift
between
excitation
(invisible
to
human
eyes)
visible
emitted
results
in
clear
stable
signal
from
immunobeads.
addition,
it
offers
possibility
site-selective
photoexcitation
process.
This
approach
not
only
meets
performance
traditional
accuracy
but
also
additional
benefits
lower
requirements
enhanced
stability,
providing
new
perspective
optimization
commercial
immunoassays.
Journal of Advanced Research,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
Staphylococcus
aureus
(S.
aureus)
is
an
important
human
pathogen
that
frequently
causes
disease
in
hospital
and
community
settings.
Due
to
the
pervasive
emergence
of
virulent
multidrug-resistant
methicillin-resistant
S.
(MRSA)
strains,
infections
have
become
a
leading
cause
morbidity
mortality.
We
aimed
develop
reliable
effective
detection
method
can
be
used
for
preventing
managing
caused
by
aureus.
A
novel
platform
combines
immunomagnetic
separation
via
beads
(IMBs)
phage
lysin
LysGH15-based
colloidal
gold
immunochromatographic
assay
(GICA)
(named
IMBs-GICA)
was
developed.
IMBs-GICA
performed
on
426
consecutive
samples,
including
185,
107,
74
60
samples
from
sputum,
urine,
bronchoalveolar
lavage
fluid
(BALF)
serum
respectively.
culture
PCR
assays
were
routinely
conducted
parallel.
By
targetingS.
aureus,
could
detect
as
little
40
CFU/mL
within
35
min.
Among
clinical
specimens,
38
positive,
36
resulting
sensitivity
89.5
%
(95
confidence
interval
74.3-96.6)
specificity
99.5
(97.9-99.9).
rapidly
sensitively
demonstrating
potential
utility
this
developed
field
testing.
Chemical Communications,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
Using
an
Ir(
iii
)-based
redox
mediator
([Ir(sppy)
3
]
3-
)
overcomes
the
traditional
kinetic
barrier
of
tri-
n
-propylamine
oxidation
on
BDD,
enhancing
ECL
from
Ru(
ii
)-labeled
beads
by
up
to
46-fold.
Analytical Sciences,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 7, 2025
Electrochemiluminescence
(ECL)
is
a
chemiluminescence
phenomenon
triggered
by
electrochemical
reactions
and
widely
used
for
(bio)chemical
analyses
bioimaging.
Compared
to
fluorescence
sensing,
ECL
sensing
reduces
background
noise
eliminating
autofluorescence
associated
with
excitation
light.
In
addition,
compared
conventional
imaging
scanning
microscopes,
faster
as
it
requires
no
scanning.
Furthermore,
unlike
electrode
arrays,
devices
can
function
without
complex
wiring,
simplifying
their
construction.
These
characteristics
render
useful
analytical
tool.
Recently,
has
been
in
vitro
cell
analysis
due
high
demand
biochips
regenerative
medicine,
drug
screening,
microphysiological
systems.
This
review
focuses
on
recent
advancements
ECL-based
applications
the
detection
of
H2O2,
respiration
activity,
adhesion,
lipid
membranes,
bipolar
electrochemistry-based
devices.
Advanced Functional Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 12, 2025
Abstract
Electrochemical
technology
provides
unique
opportunities
to
create
an
environment‐friendly
and
sustainable
chemical
industry.
Nano‐electrochemistry
offers
valuable
insights
into
the
comprehension
of
electrochemical
mechanisms
behaviors
at
nanoscale,
as
opposed
complex
ensemble
systems.
Despite
use
various
analytical
tools,
such
nanopore
scanning
probe
microscopy,
for
nano‐electrochemical
research,
direct
operando
high‐throughput
spatially
resolved
measurements
remain
challenging.
Electrochemiluminescence
(ECL)
is
reactions‐dependent
luminescence
process
without
external
light
sources.
Coupled
with
a
microscopy
configuration,
novel
imaging
methodology
ECL
(ECLM),
developed.
ECLM,
due
its
distinctive
mode,
holds
considerable
potential
in
field
nano‐electrochemistry.
This
review
will
initially
present
overview
basic
principles
ECL.
The
next
section
describe
design
ECLM
apparatus
discuss
diverse
applications
Finally,
challenges
outline
prospects
further
development
technique
summarized.
