Electrochemiluminescence Microscopy DOI Creative Commons
Sara Knežević,

Dongni Han,

Baohong Liu

et al.

Angewandte Chemie, Journal Year: 2024, Volume and Issue: 136(29)

Published: May 14, 2024

Abstract Electrochemiluminescence (ECL) is rapidly evolving from an analytical method into optical microscopy. The orthogonality of the electrochemical trigger and readout distinguishes it classic microscopy techniques, owing to its near‐zero background, remarkable sensitivity, absence photobleaching phototoxicity. In this minireview, we summarize recent advances in ECL imaging technology, emphasizing original configurations which enable biological entities improvement properties by increasing complexity multiplexing bioassays. Additionally, mapping (electro)chemical reactivity space provides valuable information on nanomaterials facilitates deciphering mechanisms for improving their performances diagnostics (electro)catalysis. Finally, highlight achievements at ultimate limits single molecules, photons or chemical reactions, current challenges translate other fields such as material science, catalysis biology.

Language: Английский

Electrochemiluminescence Microscopy DOI Creative Commons
Sara Knežević, Dongni Han, Baohong Liu

et al.

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(29)

Published: May 14, 2024

Electrochemiluminescence (ECL) is rapidly evolving from an analytical method into optical microscopy. The orthogonality of the electrochemical trigger and readout distinguishes it classic microscopy techniques, owing to its near-zero background, remarkable sensitivity, absence photobleaching phototoxicity. In this minireview, we summarize recent advances in ECL imaging technology, emphasizing original configurations which enable biological entities improvement properties by increasing complexity multiplexing bioassays. Additionally, mapping (electro)chemical reactivity space provides valuable information on nanomaterials facilitates deciphering mechanisms for improving their performances diagnostics (electro)catalysis. Finally, highlight achievements at ultimate limits single molecules, photons or chemical reactions, current challenges translate other fields such as material science, catalysis biology.

Language: Английский

Citations

27

Insulator-on-Conductor Fouling Amplifies Aqueous Electrolysis Rates DOI
Harry Morris Rodriguez, Mariusz Martyniuk, K. Swaminathan Iyer

et al.

Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: 146(15), P. 10299 - 10311

Published: April 9, 2024

The chemical industry is a major consumer of fossil fuels. Several reactions practical value proceed with the gain or loss electrons, opening path to integrate renewable electricity into manufacturing. However, most organic molecules have low aqueous solubility, causing green and cheap electricity-driven suffer from intrinsically reaction rates in industry's solvent choice: water. Here, we show that strategic, partial electrode fouling hydrophobic insulators (oils plastics) offsets kinetic limitations caused by poor reactant new for direct integration production commodity chemicals. Through electrochemiluminescence microscopy, reveal oxidation reactants up 6-fold rate increase at "fouled" oil–electrolyte–electrode interface relative clean electrolyte–electrode areas. Analogously, electrodes partially masked (fouled) plastic patterns, deposited either photolithographically (photoresists) manually (inexpensive household glues sealants), outperform electrodes. effect not limited water and, example, net gold electrodeposition are 22% larger fouled than In system involving surface-active reactant, augmentation driven synergy between insulator-confined enrichment insulator-induced current crowding, whereas only latter possibly localized decrease iR drop near insulator relevant composed non-surface-active species. Our counterintuitive design enhances electrolysis despite diminished area intimate contact introduces upscaling electrochemical processes.

Language: Английский

Citations

8

Recent advances in electrochemiluminescence immunosensing DOI Creative Commons
Jing Yu, Dalibor M. Stanković, Jasmina Vidić

et al.

Sensors & Diagnostics, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Herein, we review the recent developments and applications of ECL for immunosensing in different areas, such as detection biomarkers, bead-based bacteria cell analysis, provide future perspectives new developments.

Language: Английский

Citations

8

Nanostructured Bubbles‐Enhanced Fluorescence for Ultrasensitive Portable MicroRNA Detection DOI Open Access

Yuteng Tang,

Yuanhang Xiang, Yu Yang

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 16, 2024

Abstract The unmet on‐site clinical diagnostics are increasingly demanding portable bioassays, but compromised by sensitivity and throughput. Herein, a nanostructured bubbles‐enhanced fluorescence (nBEF) assay is reported interfacing with smartphone imaging system to enable portable, ultrasensitive, specific miRNA detection in parallel. In nBEF, the high‐curvature bubbles allow physically enhancing optical propagation, chemically fluorescence, assembling more DNA probes, undergoing faster self‐suspension efficient self‐aggregation enhancement relative their smooth counterparts. By coupling bubble‐driven signal amplification plug‐and‐play concatemer, system, this nBEF achieves ultrasensitive fluorescent of at femtomolar level one‐base resolution single test, capable monitoring drug‐induced dynamic expression accurate cancer diagnosis (AUC = 1) through profiling 40 plasma samples after RNA extraction. This further integrates an R‐value analysis RGB module realize automated, readout. Such high‐performance provides low‐cost, highly sensitive tool diagnosis.

Language: Английский

Citations

8

Quantitative immunosensor for dibenz[a,h]anthracene on-site detection in oilfield chemicals based on computer-aided antibody DOI
Jiaxun Li, Haifeng Chen, Yong Shu

et al.

Microchimica Acta, Journal Year: 2025, Volume and Issue: 192(4)

Published: March 20, 2025

Language: Английский

Citations

1

Photoinduced Electrochemiluminescence Immunoassays DOI
Dongni Han, Jasmina Vidić, Dechen Jiang

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(45), P. 18262 - 18268

Published: Nov. 1, 2024

Optimization of electrochemiluminescence (ECL) immunoassays is highly beneficial for enhancing clinical diagnostics. A major challenge the improvement operation conditions required bead-based using typical [Ru(bpy)3]2+/tri-n-propylamine (TPrA) system. In this study, we report a heterogeneous immunoassay based on near-infrared photoinduced ECL, which facilitates imaging and quantitative analysis [Ru(bpy)3]2+-modified immunobeads at low anodic potential. The photovoltage generated by photoanode under light promotes oxidation processes electrode/electrolyte interface, thus considerably lowering onset potential both TPrA ECL emission. anti-Stokes shift between excitation (invisible to human eyes) visible emitted results in clear stable signal from immunobeads. addition, it offers possibility site-selective photoexcitation process. This approach not only meets performance traditional accuracy but also additional benefits lower requirements enhanced stability, providing new perspective optimization commercial immunoassays.

Language: Английский

Citations

4

Detection of Staphylococcus aureus via IgY-Based immunomagnetic separation and a phage lysin LysGH15-Based colloidal gold immunochromatographic assay DOI Creative Commons
Xiao Liu, Zhimin Guo,

Yinghan Qiao

et al.

Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Staphylococcus aureus (S. aureus) is an important human pathogen that frequently causes disease in hospital and community settings. Due to the pervasive emergence of virulent multidrug-resistant methicillin-resistant S. (MRSA) strains, infections have become a leading cause morbidity mortality. We aimed develop reliable effective detection method can be used for preventing managing caused by aureus. A novel platform combines immunomagnetic separation via beads (IMBs) phage lysin LysGH15-based colloidal gold immunochromatographic assay (GICA) (named IMBs-GICA) was developed. IMBs-GICA performed on 426 consecutive samples, including 185, 107, 74 60 samples from sputum, urine, bronchoalveolar lavage fluid (BALF) serum respectively. culture PCR assays were routinely conducted parallel. By targetingS. aureus, could detect as little 40 CFU/mL within 35 min. Among clinical specimens, 38 positive, 36 resulting sensitivity 89.5 % (95 confidence interval 74.3-96.6) specificity 99.5 (97.9-99.9). rapidly sensitively demonstrating potential utility this developed field testing.

Language: Английский

Citations

0

Overcoming Kinetic Barriers of Remote Electrochemiluminescence on Boron-Doped Diamond via Catalytic Coreactant Oxidation DOI Creative Commons
Alessandro Fracassa, Chiara Mariani, Andrea Fiorani

et al.

Chemical Communications, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Using an Ir( iii )-based redox mediator ([Ir(sppy) 3 ] 3- ) overcomes the traditional kinetic barrier of tri- n -propylamine oxidation on BDD, enhancing ECL from Ru( ii )-labeled beads by up to 46-fold.

Language: Английский

Citations

0

Recent advances in electrochemiluminescence sensing for in vitro cell analysis: a review DOI Open Access
Kosuke Ino,

Tomas Mockaitis,

Ryota Shikuwa

et al.

Analytical Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 7, 2025

Electrochemiluminescence (ECL) is a chemiluminescence phenomenon triggered by electrochemical reactions and widely used for (bio)chemical analyses bioimaging. Compared to fluorescence sensing, ECL sensing reduces background noise eliminating autofluorescence associated with excitation light. In addition, compared conventional imaging scanning microscopes, faster as it requires no scanning. Furthermore, unlike electrode arrays, devices can function without complex wiring, simplifying their construction. These characteristics render useful analytical tool. Recently, has been in vitro cell analysis due high demand biochips regenerative medicine, drug screening, microphysiological systems. This review focuses on recent advancements ECL-based applications the detection of H2O2, respiration activity, adhesion, lipid membranes, bipolar electrochemistry-based devices.

Language: Английский

Citations

0

Electrochemiluminescence Microscopy in Nano‐Electrochemistry Research: Unraveling the Underlying Principles, Tracing the Evolutionary Developments, and Charting the Prospective Trajectories DOI Open Access

Zejing Xing,

Xuanzhao Lu, Zhichen Zhang

et al.

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

Abstract Electrochemical technology provides unique opportunities to create an environment‐friendly and sustainable chemical industry. Nano‐electrochemistry offers valuable insights into the comprehension of electrochemical mechanisms behaviors at nanoscale, as opposed complex ensemble systems. Despite use various analytical tools, such nanopore scanning probe microscopy, for nano‐electrochemical research, direct operando high‐throughput spatially resolved measurements remain challenging. Electrochemiluminescence (ECL) is reactions‐dependent luminescence process without external light sources. Coupled with a microscopy configuration, novel imaging methodology ECL (ECLM), developed. ECLM, due its distinctive mode, holds considerable potential in field nano‐electrochemistry. This review will initially present overview basic principles ECL. The next section describe design ECLM apparatus discuss diverse applications Finally, challenges outline prospects further development technique summarized.

Language: Английский

Citations

0