Circular RNA profiling reveals an abundant circPTK2 that contributes everolimus-induced endothelial cell dysfunction via regulating miR-1-5p/ACVR2B/StarD13 axis DOI
Yixin Zhao, Jiangrong Wang, Xiaomeng Jia

et al.

Acta Cardiologica, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 17

Published: May 28, 2025

mTOR inhibitors released from drug-eluting stents (DESs) play a critical role in the pathogenesis of in-stent neoatherosclerosis (ISNA), contributing to development late restenosis (ISR). Circular RNAs (circRNAs) are emerging as key regulators various pathophysiological processes, but their involvement ISNA remains unclear. The expression pattern circRNAs human umbilical vein endothelial cells (HUVECs) treated with everolimus (EVL) was analysed using RNA sequencing. levels circRNAs, miR-1-5p, and downstream targets ACVR2B/StarD13 were measured by quantitative real-time PCR. effects circPTK2 on cell proliferation, migration, apoptosis, permeability EVL-treated assessed counting kit-8, scratch, Annexin-V FITC PI double-staining, transwell assays. Bioinformatics analysis dual luciferase assay used identify interaction between miR-1-5p. association circPTK2, further evaluated functional rescue experiments. CircPTK2 significantly upregulated HUVECs. Knockdown reversed EVL-induced suppression viability reduced alleviated barrier leakage. Conversely, overexpression produced opposite effects. Mechanistically, acted sponge for leading increased its target genes ACVR2B StarD13. Silencing or StarD13 partially attenuated exacerbating miR-1-5p inhibition dysfunction. Moreover, inflammatory conditions affected expressions ACVR2B/StarD13. regulates dysfunction via miR-1-5p/ACVR2B/StarD13 axis, providing novel insights treatment ISR after DES implantation.

Language: Английский

A catalytic hairpin assembly-assisted magnetic SERS platform with core-satellite structure for ultra-sensitive circRNA detection in early lung cancer screening DOI
Luyun Xu,

Yongshi Shen,

Zhizhong Lin

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 163309 - 163309

Published: May 1, 2025

Language: Английский

Citations

0
Circular RNA profiling reveals an abundant circPTK2 that contributes everolimus-induced endothelial cell dysfunction via regulating miR-1-5p/ACVR2B/StarD13 axis DOI
Yixin Zhao, Jiangrong Wang, Xiaomeng Jia

et al.

Acta Cardiologica, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 17

Published: May 28, 2025

mTOR inhibitors released from drug-eluting stents (DESs) play a critical role in the pathogenesis of in-stent neoatherosclerosis (ISNA), contributing to development late restenosis (ISR). Circular RNAs (circRNAs) are emerging as key regulators various pathophysiological processes, but their involvement ISNA remains unclear. The expression pattern circRNAs human umbilical vein endothelial cells (HUVECs) treated with everolimus (EVL) was analysed using RNA sequencing. levels circRNAs, miR-1-5p, and downstream targets ACVR2B/StarD13 were measured by quantitative real-time PCR. effects circPTK2 on cell proliferation, migration, apoptosis, permeability EVL-treated assessed counting kit-8, scratch, Annexin-V FITC PI double-staining, transwell assays. Bioinformatics analysis dual luciferase assay used identify interaction between miR-1-5p. association circPTK2, further evaluated functional rescue experiments. CircPTK2 significantly upregulated HUVECs. Knockdown reversed EVL-induced suppression viability reduced alleviated barrier leakage. Conversely, overexpression produced opposite effects. Mechanistically, acted sponge for leading increased its target genes ACVR2B StarD13. Silencing or StarD13 partially attenuated exacerbating miR-1-5p inhibition dysfunction. Moreover, inflammatory conditions affected expressions ACVR2B/StarD13. regulates dysfunction via miR-1-5p/ACVR2B/StarD13 axis, providing novel insights treatment ISR after DES implantation.

Language: Английский

Citations

0