Sustainable Bioconversion of Methanol: A Renewable Employing Novel Alcohol Oxidase and Pyruvate Aldolase DOI Creative Commons
J. K. Kim, Yeon‐Ju Jeong, Bong Hyun Sung

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

Methanol is an ideal one-carbon (C1) feedstock for bioconversion into multicarbon value-added compounds. Biocatalytic approaches to methanol conversion provide sustainable and environmentally friendly alternatives conventional methods. This process facilitated by methanol-oxidizing enzymes, including alcohol oxidase (AOx). Here, we report AOx from Pestalotiopsis fici (PfAOx) with the highest oxidation activity efficient heterologous expression compared other AOxs. To investigate of a compound (C4 chemical, 2-keto-4-hydroxybutyrate, 2-KHB) cost-effective methanol, developed one-pot enzyme system PfAOx pyruvate aldolase Deinococcus radiodurans (DrADL) catalase Bos taurus (BtCAT, commercially available enzyme) remove toxic H2O2. The optimal reaction conditions 2-KHB production using PfAOx, DrADL, BtCAT were determined as pH 8.0, 35 °C, 0.9 mg mL-1 0.3 1.5 BtCAT, 150 mM 100 pyruvate, 5 Mg2+ shaking at 200 rpm. Under these conditions, 88.8 (10.4 g L-1) was produced 75 min, representing 74.0-fold higher yield previously reported systems pyruvate. study demonstrates promising multi-enzyme cascade approach valuable

Language: Английский

Enzyme/whole-cell biotransformation of C1 compounds into ethanolamine by using an amine transaminase from Silicibacter pomeroyi DOI

Hye-Rim Seo,

Ji-Min Woo,

Hye‐Jin Jo

et al.

Biotechnology and Bioprocess Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: March 19, 2025

Language: Английский

Citations

0
Sustainable Bioconversion of Methanol: A Renewable Employing Novel Alcohol Oxidase and Pyruvate Aldolase DOI Creative Commons
J. K. Kim, Yeon‐Ju Jeong, Bong Hyun Sung

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

Methanol is an ideal one-carbon (C1) feedstock for bioconversion into multicarbon value-added compounds. Biocatalytic approaches to methanol conversion provide sustainable and environmentally friendly alternatives conventional methods. This process facilitated by methanol-oxidizing enzymes, including alcohol oxidase (AOx). Here, we report AOx from Pestalotiopsis fici (PfAOx) with the highest oxidation activity efficient heterologous expression compared other AOxs. To investigate of a compound (C4 chemical, 2-keto-4-hydroxybutyrate, 2-KHB) cost-effective methanol, developed one-pot enzyme system PfAOx pyruvate aldolase Deinococcus radiodurans (DrADL) catalase Bos taurus (BtCAT, commercially available enzyme) remove toxic H2O2. The optimal reaction conditions 2-KHB production using PfAOx, DrADL, BtCAT were determined as pH 8.0, 35 °C, 0.9 mg mL-1 0.3 1.5 BtCAT, 150 mM 100 pyruvate, 5 Mg2+ shaking at 200 rpm. Under these conditions, 88.8 (10.4 g L-1) was produced 75 min, representing 74.0-fold higher yield previously reported systems pyruvate. study demonstrates promising multi-enzyme cascade approach valuable

Language: Английский

Citations

0