Identifying Key Properties That Drive Redox Mediator Activity in Lactiplantibacillus Plantarum DOI Creative Commons
Benjamin T. Blackburn, Robyn A. C. Alba, Vladimir Porokhin

et al.

Angewandte Chemie, Journal Year: 2025, Volume and Issue: unknown

Published: March 10, 2025

Abstract Lactiplantibacillus plantarum is known to utilize exogenous small molecule quinone mediators perform extracellular electron transfer (EET), allowing it produce a detectable current in bioelectrochemical system (BES). Utilization of by L. requires type‐II NADH dehydrogenase (Ndh2); however, structural variations the core 1,4‐naphthoquinone EET have shown yield significantly different outputs. Herein, we assembled library 40 quinone‐based probe important physicochemical properties and biochemical interactions responsible for Ndh2‐dependent . The was designed with inspiration from naturally occurring metabolites, assembly focused on modifications that diversified polarity, reduction potential, predicted free energy binding Ndh2 (ΔG comp ), as these are hypothesized drive activity. In general, activity an iron(III) nanoparticle assay correlates mediator's polarity ΔG Five were analyzed BESs , each generated over background signal. Importantly, amine‐containing mediator yielded incredibly stable output course experiment (up 5 days). These findings improve our understanding structure‐activity relationships quinone‐mediated provide bioelectronic sensing applications.

Language: Английский

Recent advances in biological approaches towards anode biofilm engineering for improvement of extracellular electron transfer in microbial fuel cells DOI Open Access
Tahseena Naaz,

Ankit Kumar,

Anusha Vempaty

et al.

Environmental Engineering Research, Journal Year: 2023, Volume and Issue: 28(5), P. 220666 - 0

Published: Jan. 10, 2023

Over the last two decades, scientific communities have been more interested in turning organic waste materials into bioenergy. Microbial fuel cells (MFC) can degrade wastewater and produce electrical power. Many constraints limited development of MFC. Among them, anode biofilm is one significant that need to be improved. This review delineates role various biological components electroactive biofilm. The current article focuses on numerous electron exchange methods for microbiome-induced transfer activity, different proteins, secretory chemicals involved transfer. study also several proteomics genomics methodologies adopted developed improve extra mechanism bacteria. Recent advances publications synthetic biology genetic engineering investigating direct indirect transport phenomena highlighted. helps reader understand recent manipulations biofilm, electrode material modifications, EET mechanisms, operational strategies improving performance. discusses challenges present technology future direction production at anode.

Language: Английский

Citations

62
Electroactive Bacteria in Natural Ecosystems and Their Applications in Microbial Fuel Cells for Bioremediation: A Review DOI Creative Commons
Gian Luigi Garbini, Anna Barra Caracciolo, Paola Grenni

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 11(5), P. 1255 - 1255

Published: May 10, 2023

Electroactive bacteria (EAB) are natural microorganisms (mainly Bacteria and Archaea) living in various habitats (e.g., water, soil, sediment), including extreme ones, which can interact electrically each other and/or with their extracellular environments. There has been an increased interest recent years EAB because they generate electrical current microbial fuel cells (MFCs). MFCs rely on able to oxidize organic matter transfer electrons anode. The latter flow, through external circuit, a cathode where react protons oxygen. Any source of biodegradable be used by for power generation. plasticity electroactive exploiting different carbon sources makes green technology renewable bioelectricity generation from wastewater rich carbon. This paper reports the most applications this promising wastewater, sediment recovery. performance terms measurements electric power), electron mechanisms EAB, MFC studies aimed at heavy metal contaminant bioremediationF all described discussed.

Language: Английский

Citations

54
Engineering extracellular electron transfer pathways of electroactive microorganisms by synthetic biology for energy and chemicals production DOI
Junqi Zhang, Feng Li,

Dingyuan Liu

et al.

Chemical Society Reviews, Journal Year: 2023, Volume and Issue: 53(3), P. 1375 - 1446

Published: Dec. 20, 2023

The excessive consumption of fossil fuels causes massive emission CO

Language: Английский

Citations

43
Integrating bioelectronics with cell-based synthetic biology DOI
Jonathan Rivnay, Ritu Raman, Jacob T. Robinson

et al.

Nature Reviews Bioengineering, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

Language: Английский

Citations

4
Electrotrophy: Other microbial species, iron, and electrodes as electron donors for microbial respirations DOI
Derek R. Lovley

Bioresource Technology, Journal Year: 2021, Volume and Issue: 345, P. 126553 - 126553

Published: Dec. 11, 2021

Language: Английский

Citations

74
Electrogenic Bacteria Promise New Opportunities for Powering, Sensing, and Synthesizing DOI Creative Commons
Seokheun Choi

Small, Journal Year: 2022, Volume and Issue: 18(18)

Published: Feb. 4, 2022

Abstract Considerable research efforts into the promises of electrogenic bacteria and commercial opportunities they present are attempting to identify potential feasible applications. Metabolic electrons from enable electricity generation sufficient power portable or small‐scale applications, while quantifiable electric signal in a miniaturized device platform can be sensitive enough monitor respond changes environmental conditions. Nanomaterials produced by offer an innovative bottom‐up biosynthetic approach synergize bacterial electron transfer create effective coupling at cell–electrode interface. Furthermore, revolutionize field bioelectronics effectively interfacing electronics with microbes through extracellular transfer. Here, these new directions for their recent integration micro‐ nanosystems comprehensively discussed specific attention toward distinct applications powering, sensing, synthesizing. challenges individual strategies solutions provided valuable guidelines practical implementation. Finally, perspective view on how use hold immeasurable promise development future presented.

Language: Английский

Citations

54
Microbe–Anode Interactions: Comparing the impact of genetic and material engineering approaches to improve the performance of microbial electrochemical systems (MES) DOI Creative Commons
Edina Klein, Melanie T. Knoll, Johannes Gescher

et al.

Microbial Biotechnology, Journal Year: 2023, Volume and Issue: 16(6), P. 1179 - 1202

Published: Feb. 18, 2023

Microbial electrochemical systems (MESs) are a highly versatile platform technology with particular focus on power or energy production. Often, they used in combination substrate conversion (e.g., wastewater treatment) and production of value-added compounds via electrode-assisted fermentation. This rapidly evolving field has seen great improvements both technically biologically, but this interdisciplinarity sometimes hampers overseeing strategies to increase process efficiency. In review, we first briefly summarize the terminology outline biological background that is essential for understanding thus improving MES technology. Thereafter, recent research at biofilm-electrode interface will be summarized discussed, distinguishing between biotic abiotic approaches. The two approaches then compared, resulting future directions discussed. mini-review therefore provides basic knowledge underlying microbiology general reviews bacteria-electrode interface.

Language: Английский

Citations

24
Microbial nanowires for sustainable electronics DOI
Matthew J. Guberman‐Pfeffer, Noémie‐Manuelle Dorval Courchesne, Derek R. Lovley

et al.

Nature Reviews Bioengineering, Journal Year: 2024, Volume and Issue: 2(10), P. 869 - 886

Published: July 4, 2024

Language: Английский

Citations

10
Enzymatic and Microbial Electrochemistry: Approaches and Methods DOI Creative Commons

Giada Bedendi,

Lilian Danielle de Moura Torquato,

Sophie Webb

et al.

ACS Measurement Science Au, Journal Year: 2022, Volume and Issue: 2(6), P. 517 - 541

Published: Aug. 29, 2022

The coupling of enzymes and/or intact bacteria with electrodes has been vastly investigated due to the wide range existing applications. These span from biomedical and biosensing energy production purposes bioelectrosynthesis, whether for theoretical research or pure applied industrial processes. Both offer a potential biotechnological alternative noble/rare metal-dependent catalytic However, when developing these biohybrid electrochemical systems, it is utmost importance investigate how approaches utilized couple biocatalysts influence resulting bioelectrocatalytic response. Accordingly, this tutorial review starts by recalling some basic principles applications bioelectrochemistry, presenting electrode biocatalyst modifications that facilitate interaction between biotic abiotic components bioelectrochemical systems. Focus then directed toward methods used evaluate effectiveness enzyme/bacteria-electrode insights they provide. concepts widely employed in enzymatic microbial electrochemistry, such as amperometry voltammetry, are initially presented later focus on various complementary spectroelectrochemistry, fluorescence spectroscopy microscopy, surface analytical/characterization techniques quartz crystal microbalance atomic force microscopy. thus aimed at students graduate approaching field while also providing critical up-to-date reference senior researchers working field.

Language: Английский

Citations

28
Systematic Full-Cycle Engineering Microbial Biofilms to Boost Electricity Production in Shewanella oneidensis DOI Creative Commons
Feng Li, Rui Tang, Baocai Zhang

et al.

Research, Journal Year: 2023, Volume and Issue: 6, P. 0081 - 0081

Published: Jan. 1, 2023

Electroactive biofilm plays a crucial rule in the electron transfer efficiency of microbial electrochemical systems (MES). However, low ability to form and conductivity formed substantially limit extracellular rate cells electrode surfaces MES. To promote formation enhance conductivity, we develop synthetic biology approach systematically engineer Shewanella oneidensis , model exoelectrogen, via modular manipulation full-cycle different stages formation, namely, from initial contact, cell adhesion, growth stable maturity dispersion. Consequently, maximum output power density engineered reaches 3.62 ± 0.06 W m −2 39.3-fold higher than that wild-type strain S. which, best our knowledge, is highest has ever been reported for biofilms genetically strains.

Language: Английский

Citations

21