Protein Design Accelerates the Development and Application of Optogenetic Tools

Computational and Structural Biotechnology Journal, Journal Year: 2025, Volume and Issue: 27, P. 717 - 732

Published: Jan. 1, 2025

Optogenetics has substantially enhanced our understanding of biological processes by enabling high-precision tracking and manipulation individual cells. It relies on photosensitive proteins to monitor control cellular activities, thereby paving the way for significant advancements in complex system research. Photosensitive play a vital role development optogenetics, facilitating establishment cutting-edge methods. Recent breakthroughs protein design have opened up opportunities develop protein-based tools that can precisely manipulate activities. These will significantly accelerate application optogenetic tools. This article emphasizes pivotal tools, offering insights into potential future directions. We begin providing an introduction historical fundamental principles followed exploration operational mechanisms key domains, which includes clarifying conformational changes they undergo response light, such as allosteric modulation dimerization processes. Building this foundation, we reveal enable creation even more sophisticated techniques.

Language: Английский

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Red light responsive Cre recombinase for bacterial optogenetics

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 31, 2024

Abstract Optogenetic tools have been used in a wide range of microbial engineering applications that benefit from the tunable, spatiotemporal control light affords. However, majority current optogenetic constructs for bacteria respond to blue light, limiting potential multichromatic control. In addition, other wavelengths offer benefits over including improved penetration dense cultures and reduced toxicity. this study, we introduce OptoCre-REDMAP, red inducible Cre recombinase system Escherichia coli . This harnesses plant photoreceptors PhyA FHY1 split version achieve precise gene expression DNA excision. We optimized design by modifying start codon characterized impact different levels induction find conditions produced minimal basal dark induced full activation within four hours exposure. system’s sensitivity ambient intensity, exposure time, finding OptoCre-REDMAP be reliable flexible across conditions. co-culture experiments with responsive OptoCre-VVD, found systems responded orthogonally inputs. Direct comparisons between OptoCre-VVD demonstrated superior properties light. OptoCre-REDMAP’s robust selective response makes it suitable advanced synthetic biology applications, particularly those requiring

Language: Английский

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Red Light Responsive Cre Recombinase for Bacterial Optogenetics

ACS Synthetic Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 19, 2024

Optogenetic tools have been used in a wide range of microbial engineering applications that benefit from the tunable, spatiotemporal control light affords. However, majority current optogenetic constructs for bacteria respond to blue light, limiting potential multichromatic control. In addition, other wavelengths offer benefits over including improved penetration dense cultures and reduced toxicity. this study, we introduce OptoCre-REDMAP, red inducible Cre recombinase system

Language: Английский

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