ACS Synthetic Biology, Journal Year: 2024, Volume and Issue: 13(11), P. 3677 - 3685
Published: Oct. 30, 2024
Ginsenosides are major active components of
Language: Английский
Biotechnology Advances, Journal Year: 2025, Volume and Issue: unknown, P. 108541 - 108541
Published: Feb. 1, 2025
Language: Английский
Citations
1
Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 12, 2025
Production of liquiritigenin, a plant-derived significant flavonoid traditionally extracted from licorice plants, is constrained by ecological and operational inefficiencies. Despite efforts to achieve heterologous reconstruction liquiritigenin synthesis pathway in microorganisms, the titers remain low process still at proof-of-concept stage, insufficient replace plant extraction. Herein, efficient production galactose induction system Saccharomyces cerevisiae was reengineered for better decoupling growth stages, making it more suitable pathways, then applied naringenin-producing strain modified redirect production. To improve ratio, dual NADPH supply developed enhance capabilities. Subsequently, concept using endogenous metabolites regulate simplification optimization natural product biosynthetic pathways proposed, general metabolic strategy model compounds, aromatic ester model, introduced. The final engineered achieved 867.67 mg/L 5 L fermenter. These results demonstrated innovative use genetic modifications overcome conventional extraction limitations, providing valuable insights synthesizing flavonoids other products.
Language: Английский
Citations
0
Systems Microbiology and Biomanufacturing, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 24, 2025
Language: Английский
Citations
0
Microbial Cell Factories, Journal Year: 2025, Volume and Issue: 24(1)
Published: Feb. 24, 2025
Metabolic engineering to increase the supply of precursors, such as 2,3-oxidosqualene (OSQ), and manipulate heterologous biosynthetic pathways through strategic overexpression multiple genes is promising for increasing microbial production triterpenoid saponins. However, use constitutive promoters, typically derived from glycolytic or ribosomal protein can cause transcription factor competition, reducing expression each gene. To avoid this issue, we overexpressed transcriptional repressor activator 1 (Rap1), known upregulate gene be involved in various metabolic pathways, including pyruvate dehydrogenase (PDH) bypass, mevalonate (MVA) pathway, sterol synthesis. Transcriptome analysis a wild-type yeast strain revealed that Rap1 significantly upregulated several central carbon metabolism (CCM)-related OSQ production, genes, particularly after diauxic shift phase. validate effect on saponin engineered Saccharomyces cerevisiae capable producing ginsenoside compound K (CK). Notably, compared with control strain, CK-producing showed upregulation controlled by TDH3 promoter, continuous precursors CK synthesis resulting 4.5-fold production. These results highlight robust strategy cell factories. Additionally, approach provides versatile framework enhancing both precursor expression.
Language: Английский
Citations
0
World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(3)
Published: Feb. 27, 2025
Language: Английский
Citations
0
Biotechnology Advances, Journal Year: 2025, Volume and Issue: 81, P. 108557 - 108557
Published: March 12, 2025
Language: Английский
Citations
0
Microorganisms, Journal Year: 2025, Volume and Issue: 13(5), P. 1067 - 1067
Published: May 3, 2025
Triterpenoids derived from Sanghuangporus baumii exhibit potent antitumor activity, but their yields under natural conditions are relatively low due to status as secondary metabolites. In this study, we investigated the effects of Zn²⁺ induction on growth and triterpenoid biosynthesis S. baumii. The results showed that 0.5 mM significantly enhanced mycelial rate (0.43 ± 0.004 cm/d) biomass (4.8 0.024 g/L), representing increases 8.71% 16.95%, respectively, compared with Zn0 group. This result was mainly caused by an increase in soluble sugar content. Furthermore, 5 induced upregulation genes mevalonate (MVA) pathway, thereby promoting accumulation 167.86% Transcriptome analysis identified SbHMGS key gene involved induction. Heterologous expression Saccharomyces cerevisiae confirmed its critical role production. (squalene) content engineered strain (Sc-HMGS) reached 0.88 mg/g induction, which 208.6% higher than non-induced control (Sc-NTC). These findings provide a foundation for optimizing industrial fermentation condition enhance yields.
Language: Английский
Citations
0
Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 163686 - 163686
Published: May 1, 2025
Language: Английский
Citations
0
Food and Chemical Toxicology, Journal Year: 2024, Volume and Issue: 196, P. 115193 - 115193
Published: Dec. 9, 2024
Language: Английский
Citations
1
Horticulture Research, Journal Year: 2024, Volume and Issue: 12(1)
Published: Oct. 6, 2024
Abstract Triterpene (C30 isoprene compounds) represents the most structurally diverse class of natural products and has been extensively exploited in food, medicine, industrial sectors. Decades research on medicinal triterpene biosynthetic pathways have revealed their roles stress tolerance shaping microbiota. However, biological function mechanism triterpenes are not fully identified. Even this scientific window narrows down for horticultural trees. The lack knowledge a scalable production system limits discovery pathways. Recent synthetic biology several important that define address many societal sustainability challenges. Here, I review chemical diversity enzymes involved biosynthesis This also outlines integrated Design-Build-Test-Learn (DBTL) pipelines discovery, characterization, optimization Further, these DBTL components share fundamental technical difficulties, highlighting opportunities interdisciplinary collaboration between researchers worldwide. advancement opens up unprecedented bioengineering compounds toward development scaleup processes.
Language: Английский
Citations
0