Mitigating Antibiotic Resistance: The Utilization of CRISPR Technology in Detection DOI Creative Commons

Xuejiao Zhang,

Z. Y. Huang,

Yanxia Zhang

et al.

Biosensors, Journal Year: 2024, Volume and Issue: 14(12), P. 633 - 633

Published: Dec. 20, 2024

Antibiotics, celebrated as some of the most significant pharmaceutical breakthroughs in medical history, are capable eliminating or inhibiting bacterial growth, offering a primary defense against wide array infections. However, rise antimicrobial resistance (AMR), driven by widespread use antibiotics, has evolved into and ominous threat to global public health. Thus, creation efficient methods for detecting genes antibiotics is imperative ensuring food safety safeguarding human The clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR-associated proteins (Cas) systems, initially recognized an adaptive immune mechanism bacteria archaea, have unveiled their profound potential sensor detection, transcending notable gene-editing applications. CRISPR/Cas technology employs Cas enzymes guides RNA selectively target cleave specific DNA sequences. This review offers extensive examination highlighting unique attributes applications antibiotic detection. It outlines current utilization progress toolkit identifying both nucleic acid (resistance genes) non-nucleic (antibiotic micromolecules) targets within field In addition, it examines challenges, such sensitivity specificity, future opportunities, including development point-of-care diagnostics, providing strategic insights facilitate curbing oversight antibiotic-resistance proliferation.

Language: Английский

Amplification-free nucleic acids detection with next-generation CRISPR/dx systems DOI
Cia‐Hin Lau, Siping Huang, Haibao Zhu

et al.

Critical Reviews in Biotechnology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 28

Published: Sept. 22, 2024

CRISPR-based diagnostics (CRISPR/Dx) have revolutionized the field of molecular diagnostics. It enables home self-test, field-deployable, and point-of-care testing (POCT). Despite great potential CRISPR/Dx in diagnoses biologically complex diseases, preamplification template often is required for sensitive detection low-abundance nucleic acids. Various amplification-free systems were recently developed to enhance signal at sufficient sensitivity. Broadly, these are classified into five groups depending on enhancement strategies employed: CRISPR/Cas12a and/or CRISPR/Cas13a integrated with: (1) other catalytic enzymes (Cas14a, Csm6, Argonaute, duplex-specific nuclease, nanozyme, or T7 exonuclease), (2) rational-designed oligonucleotides (multivalent aptamer, tetrahedral DNA framework, RNA G-quadruplexes, roller machine, switchable-caged guide RNA, hybrid locked RNA/DNA probe, hybridized cascade "U" rich stem-loop RNA), (3) nanomaterials (nanophotonic structure, gold nanoparticle, micromotor, microbeads), (4) electrochemical piezoelectric plate biosensors (SERS nanoprobes, graphene field-effect transistor, redox primer exchange reaction), (5) cutting-edge technology platforms (digital bioanalysis, droplet microfluidic, smartphone camera, single nanoparticle counting). Herein, we critically discuss advances, pitfalls future perspectives acids detection. The continued refinement will pave road rapid, cost-effective, ultrasensitive, ultraspecific on-site without resorting target amplification, with ultimate goal establishing as paragon

Language: Английский

Citations

4
Dual-Mode RPA/CRISPR-Cas12a Biosensor Based on Silica and Magnetic Hybrid Nanobeads for Rapid Detection of Campylobacter jejuni DOI
Fareeha Arshad, Anis Nadiah Abdillah, Pooja Shivanand

et al.

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

In this study, we developed a biosensor that makes use of recombinase polymerase amplification (RPA) along with CRISPR/Cas12a system integrated silica nanobeads and magnetic nanoparticle nanohybrid complex displayed peroxidase-mimicking properties. This nanozyme (NZ) integration the CRISPR/Cas allowed dual-mode fluorometric colorimetric responses . The NZ was conjugated ssDNA quencher probe sequence inherent presence target RPA amplicons, gets activated, cleaving attached to leading fluorescence signal generation. Post-CRISPR/Cas12a assay, in reaction mixture, after being cleaved away from sequence, gave colourimetric response directly proportional DNA concentration, as no longer hindered its catalytic activity. Therefore, detection using CRISPR/Cas12a-based NZ-based conferred high sensitivity selectivity toward Campylobacter detection. sensor could detect pathogenic at concentrations low 0.98 pg/μL 0.96 via absorbance spectroscopy, respectively. addition, our method also tested raw food analysis showed good recovery.

Language: Английский

Citations

0
A dual-recognition UCNPs sensor for sensitive detection of tetracycline in food using computer-designed silica-grafted paper microfluidic strategy DOI
Huanhuan Li,

Yu Wu,

Muhammad Shoaib

et al.

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: 438, P. 137799 - 137799

Published: April 17, 2025

Language: Английский

Citations

0
Recent Advances in the CRISPR/Cas-Based Nucleic Acid Biosensor for Food Analysis: A Review DOI Creative Commons
Yanan Sun,

Tianjian Wen,

Ping Zhang

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(20), P. 3222 - 3222

Published: Oct. 10, 2024

Food safety is a major public health issue of global concern. In recent years, the CRISPR/Cas system has shown promise in field molecular detection. The been coupled with various nucleic acid amplification methods and combined different signal output systems to develop new generation CRISPR/Cas-based biosensor technology. This review describes design concept its application food analysis. A detailed overview systems, methods, strategies provided. biosensors have advantages high sensitivity, strong specificity, timeliness, achieving fast analysis variety targets, including bacteria, toxins, metal ions, pesticides, veterinary drugs, adulteration, promoting development rapid detection At end, we also provide our outlook for future biosensors.

Language: Английский

Citations

1
Nanomaterials as signal amplifiers in CRISPR/Cas biosensors: A path toward multiplex point-of-care diagnostics DOI
Fareeha Arshad,

Bong Jing Yee,

Koo Pey Ting

et al.

Microchemical Journal, Journal Year: 2024, Volume and Issue: 207, P. 111826 - 111826

Published: Oct. 2, 2024

Language: Английский

Citations

1
Mitigating Antibiotic Resistance: The Utilization of CRISPR Technology in Detection DOI Creative Commons

Xuejiao Zhang,

Z. Y. Huang,

Yanxia Zhang

et al.

Biosensors, Journal Year: 2024, Volume and Issue: 14(12), P. 633 - 633

Published: Dec. 20, 2024

Antibiotics, celebrated as some of the most significant pharmaceutical breakthroughs in medical history, are capable eliminating or inhibiting bacterial growth, offering a primary defense against wide array infections. However, rise antimicrobial resistance (AMR), driven by widespread use antibiotics, has evolved into and ominous threat to global public health. Thus, creation efficient methods for detecting genes antibiotics is imperative ensuring food safety safeguarding human The clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR-associated proteins (Cas) systems, initially recognized an adaptive immune mechanism bacteria archaea, have unveiled their profound potential sensor detection, transcending notable gene-editing applications. CRISPR/Cas technology employs Cas enzymes guides RNA selectively target cleave specific DNA sequences. This review offers extensive examination highlighting unique attributes applications antibiotic detection. It outlines current utilization progress toolkit identifying both nucleic acid (resistance genes) non-nucleic (antibiotic micromolecules) targets within field In addition, it examines challenges, such sensitivity specificity, future opportunities, including development point-of-care diagnostics, providing strategic insights facilitate curbing oversight antibiotic-resistance proliferation.

Language: Английский

Citations

0