Carboxysome Shell Protein CcmK2 Assembles into Monodisperse and pH-Reversible Microparticles

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: March 31, 2025

Synthetic nano- and microparticles have become essential tools in biotechnology. Protein-based compartments offer distinct advantages over synthetic particles, such as biodegradability biocompatibility, but their development is still its infancy. Bacterial microcompartments (BMCs) are protein-based organelles consisting of a protein shell encapsulating an enzymatic core. BMCs self-assembling, selectively permeable, modular, making them ideal candidates for the Indeed, several groups engineered BMC shells individual proteins into nanoreactors functionalized molecular scaffolds. Expanding variety architectures assembled from will increase versatility building blocks Here, we developed method vitro assembly single-component monodisperse using only CcmK2, major hexameric β-carboxysome BMC. We report controlled single type solid microparticle. High-resolution imaging revealed CcmK2 particles to be assemblies radially clustered nanotubes. Through biochemical characterization, determined conditions reversible residues mediating assembly. found that pH key regulator final particle size disassembly. Our study situates precisely self-assembling future applications

Language: Английский

Pioneering Women Shaping the Field of Biocatalysis

ChemCatChem, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

Abstract In this perspective article, we celebrate the accomplishments of female‐led research groups in biocatalysis. Through initiative, aim to showcase breadth and excellence women's increase their visibility within catalysis community. The authors wish emphasize that article represents only a small selection extraordinary women who have shaped field biocatalysis over time. Among them are scientists directly or significantly influenced inspired authors’ scientific journeys.

Language: Английский