Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 4, 2024
Abstract
Copper-based
nanozyme
has
shown
the
superior
in
oxidase-like
activities
due
to
its
electron
transfer
ability
between
Cu
(I)
and
(II)
sites
during
catalytic
reactions.
Herein,
a
Cu(I)-MOF
(Cu-Mel)
was
readily
synthesized
by
traditional
hydrothermal
process
using
precursors
of
Cu+
melamine,
which
then
used
laccase-like
reactions
for
first
time.
Some
means,
such
as
X-ray
diffraction
(XRD),
scanning
microscope
(SEM),
Fourier
transform
infrared
spectroscopy
(FTIR)
photoelectron
(XPS)
were
employed
character
microstructure
Cu-Mel.
The
oxidation
4-aminoantipyrine
(4-AP)
2,4-dichlorophenol
(2,4-DP)
adopted
evaluate
resulting
conditions
including
temperatures,
presence
alcohols
ionic
concentrations,
varied
optimize
activities,
based
on
that,
highest
activity
is
presented
with
higher
maximum
reaction
rate
(Vmax).
good
storage
stability
also
Cu-Mel
utilized
degradation
Congo
red,
showing
efficiency.
These
findings
facilitate
development
laccase
mimics
serve
foundation
design
applications
Cu-MOFs
realm.
Materials,
Journal Year:
2024,
Volume and Issue:
17(8), P. 1790 - 1790
Published: April 13, 2024
In
the
present
investigation,
an
ecofriendly
magnetic
inorganic-protein
hybrid
system-based
enzyme
immobilization
was
developed
using
partially
purified
laccase
from
Trametes
versicolor
(TvLac),
Fe3O4
nanoparticles,
and
manganese
(Mn),
successfully
applied
for
synthetic
dye
decolorization
in
presence
of
inhibitors.
After
partial
purification
crude
TvLac,
specific
activity
reached
212
U∙mg
total
protein−1.
The
synthesized
Fe3O4/Mn3(PO4)2-laccase
(Fe3O4/Mn-TvLac)
Mn3(PO4)2-laccase
(Mn-TvLac)
nanoflowers
(NFs)
exhibited
encapsulation
yields
85.5%
90.3%,
respectively,
with
relative
activities
245%
260%,
compared
those
free
TvLac.
One-pot
Fe3O4/Mn-TvLac
significant
improvements
catalytic
properties
stability
to
enzyme.
retained
a
significantly
higher
residual
96.8%
over
that
Mn-TvLac
(47.1%)
after
10
reuse
cycles.
NFs
showed
potential
efficient
dyes
For
up
five
cycles,
81.1%
86.3%
Coomassie
Brilliant
Blue
R-250
xylene
cyanol,
respectively.
lower
acute
toxicity
towards
Vibrio
fischeri
than
pure
nanoparticles
did.
This
is
first
report
one-pot
synthesis
biofriendly
protein-inorganic
hybrids
TvLac
Mn.
Food Chemistry,
Journal Year:
2024,
Volume and Issue:
452, P. 139533 - 139533
Published: April 30, 2024
Encapsulating
enzymes
in
metal-organic
frameworks
is
a
common
practice
to
improve
enzyme
stability
against
harsh
conditions.
However,
the
synthesis
of
enzyme@MOFs
has
been
primarily
limited
small-scale
laboratory
settings,
hampering
their
industrial
applications.
Spray
drying
scalable
and
cost-effective
technology,
which
frequently
used
industry
for
large-scale
productions.
Despite
these
advantages,
its
potential
encapsulating
MOFs
remains
largely
unexplored,
due
challenges
such
as
nozzle
clogging
from
MOF
particle
formation,
utilization
toxic
organic
solvents,
controlled
release
encapsulated
enzymes,
high
temperatures
that
could
compromise
activity.
Herein,
we
present
novel
approach
preparing
phytase@MIL-88
A
using
solvent-free
spray
drying.
This
involves
atomizing
two
precursor
solutions
separately
three-fluid
nozzle,
with
by
manipulating
defects
within
MOFs.
The
physicochemical
properties
dried
particles
are
characterized
X-ray
diffraction,
Fourier-transform
infrared
spectroscopy,
scanning
electron
microscopy.
Leveraging
efficiency
scalability
production,
this
encapsulation
technique
holds
considerable
promise
broad
Chemical Engineering Journal,
Journal Year:
2024,
Volume and Issue:
497, P. 154471 - 154471
Published: Aug. 2, 2024
Enzyme
immobilization
within
metal–organic
frameworks
(MOFs)
addresses
the
inherent
fragility
of
enzymes,
playing
a
crucial
role
across
diverse
industries
by
improving
efficiency
and
lowering
economic
costs.
While
application
MOFs
in
food
pharmaceutical
is
constrained
toxicity
concerns,
MIL-88A(Fe)
emerges
as
an
ideal
candidate
due
to
its
non-toxicity
biocompatibility.
However,
release
encapsulated
enzymes
significantly
hampered,
reducing
their
bioactivity.
Herein,
we
present
safe
simple
platform
for
creating
enzyme@MIL-88A,
which
provides
enzyme
stabilization
controlled
release.
The
thermal
spectrum
(phytase,
xylanase,
amylase,
mannanase,
glucanase)
achieved,
elevating
endurance
threshold
95
°C.
Furthermore,
on-demand
at
target
sites
accomplished
adjusting
defects
enzyme@MIL-88A
composites
via
acid
modulation
approach,
while
preserving
activity.
This
approach
has
improved
amount
released
from
10
%
99.7
%.
To
best
our
knowledge,
this
first
time
been
synthesized
rapidly
under
mild
conditions
Our
method
offers
universal
stabilizing
vulnerable
biomaterials
delivery
biological
macromolecules.
Aggregate,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 26, 2024
ABSTRACT
Metal‐organic
frameworks
(MOFs)
are
a
new
class
of
organic‐inorganic
hybrid
materials
that
have
been
widely
studied
in
the
past
two
decades
for
their
potential
catalysis.
(1)
In
this
review,
we
comprehensively
summarize
synthesis,
application,
and
advancements
MOFs
enzyme
immobilization
mimetic
enzymes.
(2)
We
also
discuss
design
principles
behind
various
MOF‐based
biocatalysts,
such
as
enzyme@MOF
composites,
explore
utility
reactions.
(3)
Additionally,
highlight
advantages
MOF
mimetics
provide
perspective
on
solutions
to
current
challenges
(4)
Ultimately,
review
provides
general
overview
most
recent
advances
catalyst
platforms,
including
biocatalysts
nanozymes,
applications.
Journal of Agricultural and Food Chemistry,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 20, 2024
The
enzyme-linked
immunosorbent
assay
(ELISA)
stands
as
one
of
the
most
frequently
employed
rapid
detection
techniques
for
both
chemical
and
biological
contaminants.
Horseradish
peroxidase
(HRP)
hydrogen
peroxide
(H2O2),
serving
signal
generators,
play
indispensable
roles
throughout
entire
ELISA
process.
However,
HRP
H2O2
exhibit
high
sensitivity
to
elevated
temperatures,
hindering
broader
utilization
transportation
ELISA.
Hereby,
being
inspired
by
cascade
catalysis
glucose
oxidase
(GOx)
well
advantageous
properties
metal–organic
frameworks
(MOFs),
we
developed
a
catalysis-based
competitive
fluorescent
(CCF-ELISA)
mycotoxin
in
maize
using
zearalenone
(ZEN)
model.
results
revealed
remarkable
protective
effects
MOFs
on
enzymatic
activities.
Based
upon
characteristics,
built
CCF-ELISA
enabled
ZEN
with
half-maximal
inhibitory
concentration
0.54
ng/mL
allowed
range
from
0.19
1.51
ng/mL.
When
applied
naturally
contaminated
samples,
outcomes
obtained
showed
level
comparability
high-performance
liquid
chromatography–tandem
mass
spectrometry,
demonstrating
promising
potential
food
security.
ACS Nano,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 1, 2025
Protein
biomarkers
in
human
serum
provide
critical
insights
into
various
physiological
conditions
and
diseases,
enabling
early
diagnosis,
prognosis,
personalized
treatment.
However,
detecting
low-abundance
protein
is
challenging
due
to
the
presence
of
highly
abundant
proteins
that
make
up
∼99%
plasma
proteome.
Here,
we
report
use
situ
metal–organic
framework
(MOF)
growth
effectively
deplete
for
integrated
proteomic
analysis.
Through
biomolecule-mediated
nucleation
a
zeolitic
imidazolate
(ZIF-8),
are
selectively
encapsulated
within
ZIF-8
removed
from
via
centrifugation,
leaving
depleted
fraction
supernatant.
Bottom-up
proteomics
analysis
confirmed
significant
depletion
topmost
proteins,
many
at
levels
exceeding
95%.
Such
enabled
identification
277
total
supernatant
(uncaptured)
single-shot
analysis,
including
54
were
only
identified
after
depletion,
12
drug
targets,
potential
disease
biomarkers.
Top-down
characterization
captured
uncaptured
fractions
proteoform-level
this
method
not
biased
toward
any
specific
proteoform
individual
proteins.
These
results
demonstrate
MOF
can
high-abundance
simple,
low
cost,
one-pot
synthesis
enable
top-down
bottom-up
