Breath and Blood Metabolomics: A Comparative Study Using SESI-HRMS/MS and UHPLC-ESI-HRMS/MS

Environment & Health, Journal Year: 2025, Volume and Issue: unknown

Published: March 5, 2025

Breath metabolomics enables noninvasive and rapid acquisition of metabolic information by detecting volatile organic compounds (VOCs) in exhaled breath. Secondary electrospray ionization high-resolution tandem mass spectrometry (SESI-HRMS/MS) offers the highest coverage for breath metabolites among current real-time analysis techniques. Although it has been generally recognized that originate from blood, a molecular-level understanding characteristics both blood remains insufficient. In this study, nontargeted analyses samples 11 healthy volunteers were performed using SESI-HRMS/MS ultrahigh performance liquid chromatography (UHPLC-ESI-HRMS/MS), respectively. Tandem was employed metabolite annotation. Twenty-six breath-unique 73 blood-unique identified. Besides, seven found including 7-oxabicyclo [2.2.1] heptane, levulinic acid, indole, pyroglutamic malic glutamic histidine. Intriguingly, correlation these between low (r < 0.4 or p > 0.05). Among all confirmed metabolites, exhibit higher volatility according to their water–gas partition coefficient (log Pw/g) compared metabolites. addition, gender-derived differences significantly smaller than blood. summary, study indicates are likely offer complementary on When combined with metabolomics, would be advantageous appropriate application life sciences, such as biomarker discovery.

Language: Английский

Effect of PFAS serum exposure pattern on the lipid metabolism: Time to step-forward in causal inference in epidemiology

Journal of Environmental Sciences, Journal Year: 2024, Volume and Issue: 155, P. 163 - 176

Published: Dec. 28, 2024

Language: Английский