Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 11, 2024
We develop a latent biocompatible cleavage reaction involving the hitherto unexplored interaction between hydroxylamines and cyclopropenones. Our study addresses regioselectivity challenges commonly observed in asymmetric cyclopropenone transformations, substantiated by variations substrate, Density Functional Theory calculations,
Language: Английский
Chemical Communications, Journal Year: 2024, Volume and Issue: 60(51), P. 6532 - 6535
Published: Jan. 1, 2024
Polysubstituted acrylamides are ubiquitous in bioactive molecules and natural products. However, synthetic methods for the assembly of these important motifs remain underdeveloped. Herein, we report expedient synthesis structurally diverse synthetically challenging polysubstituted from readily available aromatic amines, cyclopropenones (CpOs), aryl halides
Language: Английский
Citations
0
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Oct. 24, 2024
Abstract RNA sequences encode secondary and tertiary structures that impact protein production other cellular processes. Misfolded RNAs can also potentiate disease, but the complete picture is lacking. To establish more comprehensive accurate structure-function relationships, new methods are needed to interrogate trap native conformations in environments. Existing tools primarily rely on electrophiles constitutively “on” or triggered by UV light, often resulting high background reactivity. We developed an alternative, chemically approach crosslink using bioorthogonal cyclopropenones (CpOs). These reagents selectively react with phosphines provide ketenes—electrophiles neighboring nucleophiles forge covalent crosslinks. As proof-of-concept, we synthesized a panel of CpOs appended them thiazole orange (TO-1). The TO-1 conjugates bound model aptamer (Mango) nanomolar affinity, confirmed fluorescence turn-on. After phosphine administration, crosslinks were formed between CpO probes RNA. degree crosslinking was both time dose-dependent. further applied biologically relevant conditions. Collectively, this work expands toolkit for studying its conformations.
Language: Английский
Citations
0
Angewandte Chemie, Journal Year: 2024, Volume and Issue: unknown
Published: Oct. 29, 2024
Abstract Immunoassay methods, notably enzyme‐linked immunosorbent assays (ELISAs), renowned for their signal amplification capabilities, are extensively employed in scientific research and clinical diagnostics. However, the instability of enzymes sensitivity to cellular environments present significant challenges broad application ELISA living cells. In this work, we a bioorthogonal (poly)catalysis‐linked assay (BCLISA) designed detection cell membrane antigens, which involves coupling catalysts based on small molecules or polymers antibodies. After screening, opted copper(I)‐catalyzed azide‐alkyne cycloaddition (CuAAC) as core reaction system. The polymer‐based exhibit enhanced reactivity at same molecular concentration due multiple catalytic sites. Polytriazoles formed during CuAAC have ability chelate Cu(I), thereby promoting faster catalysis. By harnessing autocatalytic feature, successfully increased potential BCLISA. Ultimately, autocatalysis‐integrated BCLISA technique was antigen imaging both vitro membranes. This approach offers new method low‐abundance antigens
Language: Английский
Citations
0
Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: unknown
Published: Oct. 29, 2024
Abstract Immunoassay methods, notably enzyme‐linked immunosorbent assays (ELISAs), renowned for their signal amplification capabilities, are extensively employed in scientific research and clinical diagnostics. However, the instability of enzymes sensitivity to cellular environments present significant challenges broad application ELISA living cells. In this work, we a bioorthogonal (poly)catalysis‐linked assay (BCLISA) designed detection cell membrane antigens, which involves coupling catalysts based on small molecules or polymers antibodies. After screening, opted copper(I)‐catalyzed azide‐alkyne cycloaddition (CuAAC) as core reaction system. The polymer‐based exhibit enhanced reactivity at same molecular concentration due multiple catalytic sites. Polytriazoles formed during CuAAC have ability chelate Cu(I), thereby promoting faster catalysis. By harnessing autocatalytic feature, successfully increased potential BCLISA. Ultimately, autocatalysis‐integrated BCLISA technique was antigen imaging both vitro membranes. This approach offers new method low‐abundance antigens
Language: Английский
Citations
0
Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 11, 2024
We develop a latent biocompatible cleavage reaction involving the hitherto unexplored interaction between hydroxylamines and cyclopropenones. Our study addresses regioselectivity challenges commonly observed in asymmetric cyclopropenone transformations, substantiated by variations substrate, Density Functional Theory calculations,
Language: Английский
Citations
0