Photoactivatable Plasma Membrane Probe Through Self-Triggered Photooxidation Cascade for Live Super-Resolution Microscopy DOI Creative Commons
Sonia Pfister, Valentine Le Berruyer, Kyong Tkhe Fam

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 30, 2024

Abstract Super-resolution imaging based on the localization of single emitters requires a spatio-temporal control ON and OFF state. To this end, photoactivatable fluorophores are adapted as they can be turned upon light irradiation. Here we present concept called Self-Triggered Photooxidation Cascade (STPC) photooxidation plasma membrane targeted leuco-rhodamine (LRhod-PM), non-fluorescent reduced form rhodamine probe. Upon visible irradiation small number oxidized rhodamines, Rhod-PM, acts photosensitizer to generate singlet oxygen capable oxidize state LRhod-PM. We showed that phenomenon is kinetically favored by high local concentration propagates quickly when probe embedded in bilayers. In addition, close proximity dyes favors photobleaching. At single-molecule level, concomitant activation/bleaching phenomena allow reaching blinking regime enabling microscopy for super-resolution live cellular membranes.

Language: Английский

Thioketal-photocage: a universal modification strategy for constructing new photochemical tools for real-time imaging in living cells DOI

Ding‐Heng Zhou,

Shanyong Chen, Yan-Zhao Liu

et al.

Science Bulletin, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Endogenous Free-Electron-Involved Coreactant-Free Electrochemiluminescence from Nanoclusters and Its Immunoassay Application DOI
Yaojia Ai, Xuwen Gao, Xiaowen Xu

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

All of the commercialized electrochemiluminescence (ECL) immunoassays are automatically conducted at +1.40 V (vs Ag/AgCl) in coreactant route. To alleviate exogenous effect coreactants and simplify operation procedures, herein, a sulfur-vacancy-involved free electron strategy is proposed to exploit Au nanoclusters (NCs) as anodic electrochemiluminophores perform coreactant-free immunoassay. The deficient coordination between sulfhydryl Met core might induce departure partial S atoms enable Met-capped AuNCs (Met-AuNCs) with electron-rich nature. nature tends endow Met-AuNCs unpaired endogenous electrons, which can directly combine holes for light emitting. Coreactant-free ECL around +0.86 consequently conveniently achieved by merely oxidizing anode. qualified determine human carcinoembryonic antigen from 10 5000 pg/mL limit detection 5 pg/mL. Electron paramagnetic resonance provides clear evidence that electrons within play an important role generation ECL. This promising designing nanoelectrochemiluminophores improved immunoassay performance.

Language: Английский

Citations

0
In Situ Lighting-Up Near-Infrared Metabolic Probes for Super-Resolution Imaging of Glycogen DOI
Dan Li,

Ruilong Dai,

Shangjun Chen

et al.

ACS Sensors, Journal Year: 2025, Volume and Issue: unknown

Published: March 10, 2025

High-fidelity tracking of glycogen dynamics in living biosystems is critical for exploring the biological role metabolism diseases. However, situ information on state mainly relies a glucose uptake fluorescence probe 2-NBDG, which has proven to be extremely limited owing "always-on" fluorescence, short emission wavelength, and low signal-to-noise (S/N) ratio. Herein, we first time demonstrate metabolic-activated off-on through covalently attaching molecular rotor with hydrophilic at C-2 position guarantee good miscibility complete fluorescence-off before metabolic incorporation into particles. The Glycogen-Red achieves negligible background (1/30 times than 2-NBDG) an ultrahigh S/N ratio (61-fold lighting-up near-infrared (NIR) biosynthesis. Notably, our unique characteristic bypassing washing steps, offering powerful toolbox real-time biosynthesis super-resolution mapping structures cells.

Language: Английский

Citations

0
Advancing Single-Molecule Biophysics: Next-Generation Organic Fluorophores with Tailored Labeling Strategies DOI Creative Commons
Lei Zhang,

Dongwen Shen,

Jia-Zhen Yang

et al.

Chemical & Biomedical Imaging, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Bright photoactivatable probes based on triphenylethylene for Cu2+ detection in tap water and tea samples DOI
Zuo‐Qin Liang, Dongdong Song,

Zhuo-Cheng Li

et al.

Food Chemistry, Journal Year: 2023, Volume and Issue: 434, P. 137439 - 137439

Published: Sept. 15, 2023

Language: Английский

Citations

10
Ether Rhodamines with Enhanced Hydrophilicity, Fluorogenicity, and Brightness for Super-Resolution Imaging DOI Open Access

Xiangning Fang,

Qinglong Qiao,

Z W Li

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 2, 2025

ABSTRACT Rhodamine dyes are widely used fluorophores in super-resolution fluorescence imaging due to their exceptional optical properties and “aggregation-disaggregation” induced fluorogenic activation. However, excessive lipophilicity often reduces brightness aqueous environments causes off-target staining, limiting effectiveness high-resolution imaging. To address these challenges, we introduce an ether-decorated N-terminal modification strategy for rhodamine silicon-rhodamine (Si-rhodamine), replacing conventional N-alkyl groups. The ether chains enhance water solubility, decrease aggregate size, improve fluorogenicity across a wide concentration range. Their flexible, hydrophilic structure forms protective shield around the xanthene core, minimizing dye-water interactions reducing quenching. Additionally, inductive effect of decreases electron-donating strength amino groups, suppressing quenching caused by twisted intramolecular charge transfer (TICT). These modifications collectively increase quantum yields ER ESiR from 0.35 0.19 (for tetraethyl-substituted analogs) 0.70 0.41, respectively. Probes derived exhibit outstanding fluorogenicity, enhanced signal-to-noise ratios, improved resolution complex environments, demonstrating superior performance advanced techniques such as structured illumination microscopy (SIM), stimulated emission depletion (STED) microscopy, single-molecule localization (SMLM). This work introduces innovative fluorophore design, offering significant advancements applications.

Language: Английский

Citations

0
A furo-fused coumarin dye with large Stokes shift and intensive emission intensity: its design, synthesis, characterizaiton, optical properties and solvatochromic applications DOI

Liai Bai,

Mengmeng Zhang, Yanyi Huang

et al.

Journal of Molecular Structure, Journal Year: 2025, Volume and Issue: unknown, P. 142319 - 142319

Published: April 1, 2025

Language: Английский

Citations

0
Photo-uncaging Triggers on Self-Blinking to Control Single-Molecule Fluorescence Kinetics for Super-resolution Imaging DOI
Ying Zheng, Zhiwei Ye, Xue Zhang

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(28), P. 18477 - 18484

Published: June 28, 2024

Super-resolution imaging, especially a single-molecule localization approach, has raised fluorophore engineering revolution chasing sparse dark-bright blinking transforms. Yet, it is challenge to structurally devise fluorophores manipulating the kinetics. In this pursuit, we have developed triggering strategy by innovatively integrating photoactivatable nitroso-caging into self-blinking sulfonamide form nitroso-caged rhodamine (NOSR). Our demonstrated controllable events upon phototriggered caging unit release. This exceptional blink kinetics improved super-resolution imaging integrity on microtubules compared analogues. With aid of paramount fluorescence kinetics, successfully reconstructed ring structure nuclear pores and axial morphology mitochondrial outer membranes. We foresee that our synthetic approach photoactivation would facilitate devising for imaging.

Language: Английский

Citations

2
Unprecedented perspectives on the application of CinNapht fluorophores provided by a “late-stage” functionalization strategy DOI Creative Commons

Eléonore Tacke,

Minh‐Duc Hoang,

Kevin Tatoueix

et al.

Chemical Science, Journal Year: 2023, Volume and Issue: 14(22), P. 6000 - 6010

Published: Jan. 1, 2023

A simple and easy-to-implement process based on a nucleophilic aromatic substitution reaction with wide variety of nucleophiles fluorinated CinNapht offers unprecedented perspectives.

Language: Английский

Citations

6
Dual‐Color Photoconvertible BODIPY Dyes Based on Visible‐Light Photouncaging of Nitrobenzyl Moieties for Living‐Cell Imaging DOI Open Access
Heng Li, Qi Zhang, Xing Guo

et al.

Advanced Optical Materials, Journal Year: 2023, Volume and Issue: 12(6)

Published: Oct. 20, 2023

Abstract Photoactivatable fluorophores are useful tools in live cell imaging due to precise spatial and temporal control. Herein, a tandem visible‐light photodecaging tautomerization strategy is proposed as an activation mechanism for the construction of photoconvertible boron dipyrromethenes (BODIPY) dyes, from which nitro‐benzyloxy BODIPY derivatives developed new class visible light photoactivated fluorophores. Upon exposure light, moiety released converted keto‐form analog by tautomerization. The photoconversion process not only achieves over 50 nm blue shift absorption/emission maxima but also provides bright fluorescence both pre‐ post‐activation forms. Mechanism studies indicate that no indications radical species singlet oxygen involved this unique UV irradiation can efficiently activate photoinduced release nitrobenzyl alcohol through photoexciting core rather than classical photocage. Cell vesicle successfully performed using dyes. results show these dyes highly efficient, universal, non‐cytotoxic, valuable development promising molecules.

Language: Английский

Citations

5