Langmuir,
Journal Year:
2024,
Volume and Issue:
40(43), P. 22794 - 22802
Published: Oct. 16, 2024
Enhancing
the
structural
stability
of
an
enzyme
and
maintaining
its
catalytic
activity
are
effective
ways
to
improve
utilization
reduce
cost
drug
screening.
However,
immobilized
tends
decrease
in
existing
immobilization
techniques
due
conformational
changes
microenvironmental
restrictions.
In
this
paper,
we
present
a
facile
approach
prepare
acetylcholinesterase
(AChE)
with
high
by
ZIF-8
situ
citric
acid
(CA)
etching
strategy.
CA
breaks
coordination
bond
produces
defects,
expanding
pore
space,
improving
substrate
accessibility,
fully
exposing
active
site
enzyme.
The
enhancement
AChE@ZIF-8-CA
was
about
6.10-fold
compared
free
addition,
exhibited
excellent
encapsulation
efficiency
good
tolerance
temperature,
pH,
organic
solvents.
relative
remains
at
initial
83.77%
even
five
repeated
experiments.
strategy
provides
novel
efficient
way
quickly
construct
highly
enzymes
under
mild
conditions.
ACS Central Science,
Journal Year:
2024,
Volume and Issue:
10(2), P. 358 - 366
Published: Jan. 18, 2024
Encapsulating
enzymes
within
metal–organic
frameworks
has
enhanced
their
structural
stability
and
interface
tunability
for
catalysis.
However,
the
small
apertures
of
restrict
effectiveness
to
organic
molecules.
Herein,
we
present
a
green
strategy
directed
by
visible
linker
micelles
aqueous
synthesis
MAF-6
that
enables
catalytic
asymmetric
chiral
Due
large
pore
aperture
(7.6
Å),
double
size
benchmark
ZIF-8
(3.4
allows
encapsulated
enzyme
BCL
access
larger
substrates
do
so
faster.
Through
optimization
surfactants'
effect
during
synthesis,
BCL@MAF-6-SDS
(SDS
=
sodium
dodecyl
sulfate)
displayed
efficiency
(Kcat/Km)
was
420
times
greater
than
BCL@ZIF-8.
This
biocomposite
efficiently
catalyzed
drug
precursor
molecules
with
94–99%
enantioselectivity
nearly
quantitative
yields.
These
findings
represent
deeper
understanding
de
novo
synthetic
encapsulation
in
MOFs,
thereby
unfolding
great
potential
enzyme@MAF
catalysts
organics
pharmaceuticals.
ACS Materials Letters,
Journal Year:
2024,
Volume and Issue:
6(7), P. 2609 - 2616
Published: May 28, 2024
The
combination
of
photocatalysis
and
biocatalysis
in
a
heterogeneous
fashion
for
enantioselective
catalysis
is
great
importance
but
still
underexplored.
Herein,
we
rationally
design
construct
photoenzymatic
platform
catalysis,
by
mechanochemical
synthesis
strategy
to
simultaneously
encapsulate
photocatalyst
WGL
into
robust
Zr-based
MOF.
Due
the
rapid
minimizing
use
organic
solvents
strong
acid,
fabricated
photobiocatalysts
show
high
photobiological
activity
operational
stability,
hence
affording
good
performance
reusability
asymmetric
Mannich
reactions.
These
wide
substrate
scope
(up
12
substrates)
are
first
used
N-aryl-substituted
tetrahydroisoquinoline
substrates,
which
have
not
yet
been
achieved
photobiocatalysis
yet.
Furthermore,
various
characterization
techniques
unveil
superoxide
anions
singlet
oxygen
as
main
active
substances
catalysis.
This
research
marks
pivotal
step
toward
crafting
range
enzyme-MOF
tailored
diverse
applications
across
industrial
processes.
JACS Au,
Journal Year:
2024,
Volume and Issue:
4(8), P. 3170 - 3182
Published: Aug. 12, 2024
In
this
study,
we
present
the
first
example
of
using
a
machine
learning
(ML)-assisted
design
strategy
to
optimize
synthesis
formulation
enzyme/ZIFs
(zeolitic
imidazolate
framework)
for
enhanced
performance.
Glucose
oxidase
(GOx)
and
horseradish
peroxidase
(HRP)
were
chosen
as
model
enzymes,
while
Zn(eIM)2
(eIM
=
2-ethylimidazolate)
was
selected
ZIF
test
our
ML-assisted
workflow
paradigm.
Through
an
iterative
ML-driven
training-design-synthesis-measurement
workflow,
efficiently
discovered
GOx/ZIF
(G151)
HRP/ZIF
(H150)
with
their
overall
performance
index
(OPI)
values
(OPI
represents
product
encapsulation
efficiency
(E
in
%),
retained
enzymatic
activity
(A
thermal
stability
(T
%))
at
least
1.3
times
higher
than
those
systematic
seed
data
studies.
Furthermore,
advanced
statistical
methods
derived
from
trained
random
forest
qualitatively
quantitatively
reveal
relationship
among
synthesis,
structure,
enzyme/ZIF
system,
offering
valuable
guidance
future
studies
on
enzyme/ZIFs.
Overall,
proposed
holds
promise
accelerating
development
other
enzyme
immobilization
systems
biocatalysis
applications
beyond,
including
drug
delivery
sensing,
others.
