Multiscale photocatalytic proximity labeling reveals cell surface neighbors on and between cells

Science, Journal Year: 2024, Volume and Issue: 385(6706)

Published: July 18, 2024

Proximity labeling proteomics (PLP) strategies are powerful approaches to yield snapshots of protein neighborhoods. Here, we describe a multiscale PLP method with adjustable resolution that uses commercially available photocatalyst, Eosin Y, which upon visible light illumination activates different photo-probes range radii. We applied this platform profile neighborhoods the oncogenic epidermal growth factor receptor and orthogonally validated more than 20 neighbors using immunoassays AlphaFold-Multimer prediction. further profiled cell-cell synapses induced by bispecific T cell engagers chimeric antigen cells. This integrated maps local distal networks on between surfaces, will aid in systematic construction surface interactome, revealing horizontal signaling partners reveal new immunotherapeutic opportunities.

Language: Английский

---

Robust Miniemulsion PhotoATRP Driven by Red and Near-Infrared Light

Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: 146(19), P. 13417 - 13426

Published: May 1, 2024

Photoinduced polymerization techniques have gathered significant attention due to their mild conditions, spatiotemporal control, and simple setup. In addition homogeneous media, efforts been made implement photopolymerization in emulsions as a practical greener process. However, previous photoinduced reversible deactivation radical (RDRP) heterogeneous media has relied on short-wavelength lights, which limited penetration depth, resulting slow relatively poor control. this study, we demonstrate the first example of highly efficient miniemulsion ATRP open air driven by red or near-infrared (NIR) light. This was facilitated utilization water-soluble photocatalyst, methylene blue (MB+). Irradiation red/NIR light allowed for excitation MB+ subsequent photoreduction deactivator presence electron donors initiate mediate The NIR light-driven photoATRP provided successful synthesis polymers with low dispersity (1.09 ≤ Đ 1.29) quantitative conversion within an hour. study further explored impact kinetics reactors varying sizes large-scale reaction (250 mL), highlighting advantages longer-wavelength light, particularly dispersed owing its superior penetration. work opens new avenues robust emulsion techniques, offering more approach improved control efficiency.

Language: Английский

---

Low-energy photoredox catalysis

Nature Reviews Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 11, 2024

Language: Английский

---

Synthesis, Characterization, and Catalytic Activity of Ni(0) (DQ)dtbbpy, an Air-Stable, Bifunctional Red-Light-Sensitive Precatalyst

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

Despite a well-established and growing body of work on nickel(0) precatalysts, the potential complexes as bifunctional precatalysts remains underexplored. In this study, we synthesized, characterized, evaluated catalytic activity (Ni(0)(DQ)dtbbpy), bifunctional, red-light-sensitive, air-stable complex. Owing to its unique photophysical properties, it effectively catalyzed etherification amination aryl bromides under 620–630 nm light irradiation, functioning both photocatalyst an active metal catalyst. Mechanistic studies density functional theory (DFT) calculations further confirmed exceptional absorption properties Ni(0)(DQ)dtbbpy in red-light region, well electron transfer process triggered by irradiation.

Language: Английский

---

Recent advancements in the molecular design of deep-red to near-infrared light-absorbing photocatalysts

Chem Catalysis, Journal Year: 2024, Volume and Issue: 4(8), P. 100973 - 100973

Published: April 17, 2024

Language: Английский

---

Deep-Red and Ultrafast Photocatalytic Proximity Labeling Empowered In Situ Dissection of Tumor-Immune Interactions in Primary Tissues

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Immunotherapy efficacy in solid tumors varies greatly, influenced by the tumor microenvironment (TME) and dynamic tumor-immune interactions within it. Decoding these situ with minimal interference native tissue architecture delicate immune responses is critical for understanding progression optimizing therapeutic strategies. Here, we introduce CAT-Tissue, a novel deep-red photocatalytic proximity labeling method that enables ultrafast, high-resolution profiling of primary tissues. By leveraging nanobody-Chlorin e6 as photocatalyst biotin-aniline probe, CAT-Tissue enabled rapid comprehensive detection various both coculture systems sections. Coupled bulk RNA-sequencing, revealed distinct gene expression patterns between tumor-neighboring tumor-distal lymphocytes, highlighting recognition CD8+ T cells, which exhibited activated, effector, exhausted phenotypes. cell strategy excellent penetration biocompatibility, offers nongenetically encoded platform high sensitivity spatiotemporal controllability complex environments situ, may advance our immunology guide development more effective immunotherapies.

Language: Английский

---

Multiprobe Photoproximity Labeling of the EGFR Interactome in Glioblastoma Using Red-Light

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: March 7, 2025

Photocatalytic proximity labeling has emerged as a valuable technique for studying interactions between biomolecules in cellular context, providing precise spatiotemporal control over protein labeling. One significant advantage of these methods is their modularity, allowing the use single photocatalyst with different reactive probes to expand interactome coverage and capture diverse interactions. Despite advances, fewer have been developed using red-light excitation, limiting photoproximity more complex media such tissues animal models. Herein, we develop platform under utilizing catalyst two distinct probe types. We first design carbene based system that utilizes sulfonium diazo probes. This successfully applied on A549 cells epidermal growth factor receptor (EGFR) Cetuximab-Chlorin e6 conjugate. Benchmarking against established techniques indicates this approach performs comparably leading carbene-based methods. Next, leverage strong singlet oxygen generation (SOG) ability Chlorin establish an alternative aniline hydrazide EGFR directed chemoproteomics experiments reveal overlap system, capturing subset identified by SOG system. Finally, deploy our characterization resected human glioblastoma (GBM) tissue samples removed from locations same tumor, representing tumor's infiltrating edge its viable center, identifying several GBM specific interacting proteins may serve launch point future therapeutic campaigns.

Language: Английский

---

Nitrogen-Based Organofluorine Functional Molecules: Synthesis and Applications

Chemical Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

Fluorine and nitrogen form a successful partnership in organic synthesis, medicinal chemistry, material sciences. Although fluorine-nitrogen chemistry has long rich history, this field received increasing interest made remarkable progress over the past two decades, driven by recent advancements transition metal organocatalysis photochemistry. This review, emphasizing contributions from 2015 to 2023, aims update state of art synthesis applications nitrogen-based organofluorine functional molecules chemistry. In dedicated sections, we first focus on fluorine-containing reagents organized according type groups attached nitrogen, including N-F, N-RF, N-SRF, N-ORF. review also covers nitrogen-linked building blocks, catalysts, pharmaceuticals, agrochemicals, underlining these components' broad applicability growing importance modern

Language: Английский

---

Diazirine-Functionalized Polyurethane Crosslinkers for Isocyanate-Free Curing of Polyol-Based Coatings

ACS Applied Polymer Materials, Journal Year: 2024, Volume and Issue: 6(6), P. 3517 - 3522

Published: March 5, 2024

Polyurethane coatings have strong material properties due to the hydrogen bonding inherent urethane groups. However, installing this moiety usually requires curing through difficult-to-handle isocyanates. In work, we show development of a polyurethane-based crosslinker that can be used formulate one-component polyurethane coating with similar those isocyanate-based coatings. To achieve this, diazirine functionalities generate carbenes upon heating, which react alcohol in polyol crosslinked network high storage modulus.

Language: Английский

---

Near-infrared light activatable nanoplatform for proteins labeling, enrichment and visualization in living systems

Chinese Chemical Letters, Journal Year: 2025, Volume and Issue: unknown, P. 110966 - 110966

Published: Feb. 1, 2025

Language: Английский

---