Natural Size Variation Amongst Protocells Leads to Survival and Growth Under Hypoosmotic Conditions DOI
Lauren A. Lowe,

Natasha Kaushik,

Anna Wang

et al.

Small, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 4, 2024

Abstract Membrane growth is vital to the evolution of cellular life. For model protocells, this typically achieved through competition between different protocell populations or by adding extra amphiphiles. This work demonstrates an alternative mechanism for membrane growth: hypoosmotic shocks, which could have occurred naturally in environment, leading redistribution lipids within a single population vesicles. Here we report that, even without additional lipid supply, nanoscale and giant fatty acid vesicles can withstand substantial osmotic pressures growth, whilst also retaining significant portion their contents. stands contrast phospholipid systems, burst release contents under same conditions. Notably, retained following shocks ten times higher than predicted tolerance levels. The observed robustness likely enabled membrane's incorporation amphiphiles from smaller less tense population. dynamic nature these acid‐based protocells not only supports but enables survival environments.

Language: Английский

Water Transportation through Nano/Microsized Lipid Protocells with a Significant Deviation from the van’t Hoff Osmotic Rule DOI

Shujiao Chen,

Shuai Zhang, Qunhui Yuan

et al.

The Journal of Physical Chemistry B, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

Osmotic pressure is known to be an important driving force that induces water transport through membranes, which crucial for many biophysical processes. Here, we observed under a relatively low osmotic induced by sugars' protocells (vesicles) with diameter of ∼110 nm barely shrank. However, NaCl and CaCl2 at lower concentrations rapid decrease in the vesicle size as evidence transportation membrane. An additional mechanical resulting from increase interfacial tension lipid membrane was proposed main this electrolyte-specific effect. These results indicate not only nano/microsized protocells.

Language: Английский

Citations

0
Apolipoprotein interaction induces shape remodeling and lipid phase separation in giant unilamellar vesicles DOI Creative Commons

Christopher F. Carnahan,

Wei He, Tuğba N. Öztürk

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

ABSTRACT Apolipoprotein A-I (ApoA-I) – a 243-residue amphipathic protein containing an N-terminal globular domain and primarily helical C-terminal lipid binding is principal component of high-density lipoprotein (HDL) or “good” cholesterol, which important homeostasis in humans. Synthesized the liver intestine excreted blood, ApoA-I undergoes complex, cooperative, dynamic self-assembly with membrane lipids producing unlipidated (or weakly lapidated), nascent discoidal, mature HDL states. In vitro studies establish that recombination purified reconstitutes this cooperative self-assembly. However, kinetic pathways by these mesoscopic, proteolipidic assemblies form remain incompletely understood. Here, we monitor dynamics ApoA-I-membrane interactions through real-time monitoring morphological changes, ensue when incubated minimal giant unilamellar vesicles (GUVs) composed single phospholipids phase-separating phospholipid-cholesterol mixtures. Our fluorescence atomic force microscopy measurements reveal interaction produces discoidal particles while remodeling parent vesicle discrete stages involving poration, solute leakage, vesiculation, lipid-lipid phase separation. We find qualitative phenomenology robust fully reproducible for different mutants alleles (WT APOA-1, Δ49ApoA-I, ApoE-3, ApoE-4) mixtures (PC, PC+PS), tested. molecular simulations recapitulate essential shape changes further composition dependence interactions. Together, findings depict key steps protein-lipid interactions, guide assembly mesoscopic reconstituted lipoproteins nanodiscs.

Language: Английский

Citations

0
Natural size variation amongst protocells leads to survival and growth under hypoosmotic conditions DOI Creative Commons
Lauren A. Lowe,

Natasha Kaushik,

Anna Wang

et al.

Published: June 18, 2024

Membrane growth is vital to the evolution of cellular life. For model protocells, this has most commonly been shown occur through competition between different protocell populations or with addition extra amphiphiles. We find an alternative mechanism for membrane that occurs as a consequence hypoosmotic shocks could have occurred naturally in environment. show nanoscale and giant fatty acid vesicles can withstand substantial osmotic pressures growth, whilst also retaining significant portion their contents. Notably, retained contents following ten times higher than predicted be tolerable. This likely enabled by membrane’s incorporation additional amphiphiles from less tense vesicles. The dynamic nature these acid-based protocells provides which not only occurs, but enables survival environments.

Language: Английский

Citations

0
Reactivation of nucleases with peroxidation damages induced by a menadione: ascorbate combination devastates human prostate carcinomas: ultrastructural aspects DOI Creative Commons
Jacques Gilloteaux,

James M. Jamison,

Jack L. Summers

et al.

Ultrastructural Pathology, Journal Year: 2024, Volume and Issue: 48(5), P. 378 - 421

Published: Aug. 6, 2024

Introduction Xenografts of androgen-independent human DU145 prostate metastatic carcinomas implanted in nu/nu male mice have revealed a significant survival after prooxidant anticancer treatment consisting combination menadione bisulfite and sodium ascorbate (VK3:VC).

Language: Английский

Citations

0
Osmotic spawning vesicle DOI Creative Commons
Minoru Kurisu, Masayuki Imai

Soft Matter, Journal Year: 2024, Volume and Issue: 20(45), P. 8976 - 8989

Published: Jan. 1, 2024

We discovered a novel division mechanism of vesicles. This simple and highly productive system will provide proliferation ability to existing future microcompartment systems, simply by selecting suitable membrane compositions osmolytes.

Language: Английский

Citations

0
Invagination of Giant Unilamellar Vesicles upon Membrane Mixing with Native Vesicles DOI Creative Commons

Garvita Dhanawat,

Manorama Dey,

Anirudh Singh

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(46), P. 46615 - 46626

Published: Nov. 7, 2024

We demonstrate rapid membrane mixing between GUVs of pure lipid compositions and vesicles (MVs) isolated from the plasma Vero cells, resulting in transfer native lipids proteins to GUVs. The steps involved are docking followed by fusion. show that positively charged essential for docking, components MVs drive interleaflet lateral asymmetry a change tension upon trigger invagination. detected outward inward invagination sites at rim within 10-40 min mixing. extent invaginations depends on cholesterol sphingomyelin (SM) contents Cholesterol content above critical concentration disfavors invaginations, SM is an molecular factor

Language: Английский

Citations

0
Natural Size Variation Amongst Protocells Leads to Survival and Growth Under Hypoosmotic Conditions DOI
Lauren A. Lowe,

Natasha Kaushik,

Anna Wang

et al.

Small, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 4, 2024

Abstract Membrane growth is vital to the evolution of cellular life. For model protocells, this typically achieved through competition between different protocell populations or by adding extra amphiphiles. This work demonstrates an alternative mechanism for membrane growth: hypoosmotic shocks, which could have occurred naturally in environment, leading redistribution lipids within a single population vesicles. Here we report that, even without additional lipid supply, nanoscale and giant fatty acid vesicles can withstand substantial osmotic pressures growth, whilst also retaining significant portion their contents. stands contrast phospholipid systems, burst release contents under same conditions. Notably, retained following shocks ten times higher than predicted tolerance levels. The observed robustness likely enabled membrane's incorporation amphiphiles from smaller less tense population. dynamic nature these acid‐based protocells not only supports but enables survival environments.

Language: Английский

Citations

0