Cited by Scalable design of uniform oligourethanes for impact study of chain length, sequence and end groups on thermal properties

Chemical Synthesis and Poly(ethylene glycol)-Like Conjugation of the Mango-II Fluorogenic RNA Aptamer DOI

Maria Nerantzaki, Claire Husser, Isaure Sergent

et al.

Bioconjugate Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 20, 2025

A reliable method for the efficient chemical synthesis and poly(ethylene glycol) PEG-like modification of fluorogenic RNA aptamers is reported. The 43-mer version Mango-II (MangoII-v1), which binds tightly specifically to green fluorophore TO1-Biotin (TO1-B), was synthesized by automated phosphoramidite chemistry using 2'-O-[(triisopropylsilyl)oxy]methyl] (2'-O-TOM)-protected ribonucleosides. Solid-phase also used as a single tool prepare MangoII-v1 modified with oligophosphate synthetic segment (MangoII-v1-P). After cleavage from resin, deprotection, purification, capacity activate fluorescence TO1-B degradation behavior chemically RNAs MangoII-v1-P, were deeply investigated in comparison those enzymatically 48 nucleotides long MangoII. Interestingly, aptamer demonstrated great activity toward its target, compared analogue. Moreover, it found be highly stable, retaining structural integrity bioactivity, even after seven days incubation 20% fetal bovine serum. MangoII-v1-P showed high affinity excellent resistance.

Language: Английский

Citations

New Wine in Old Bottle: Crown Ether-Functionalized Digital Polymer toward Efficient MALDI-TOF MS/MS Decoding via a Classical Supramolecular Interaction DOI

Xiaoman Huang,

Zhilin Yang,

Xiaojie Yang

et al.

ACS Macro Letters, Journal Year: 2024, Volume and Issue: 13(8), P. 979 - 986

Published: July 23, 2024

Digital polymers (DPs), which serve as promising molecular-level storage media, have increasingly garnered interest. Their application significantly depends on the efficiency of information writing (synthesis) and reading processes (sequencing). For reading, rational incorporation weak bonds in main chain was applied most cases order to improve readability tandem mass spectra (MS/MS), would limit length DPs, thus reducing capacity. In this study, introduction commercially available crown ether (CE) at terminus digital oligo(γ-butyrolactone)s (DOBLs) enhances predictability fidelity matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF MS/MS), improving decoding process. The use ether, leveraging a well-established supramolecular interaction with alkali cations known since 1967, offers strong affinity between ionization agents CE motifs, form selective effect desired fragments during MS. This method is particularly effective for long-chain extending up 32-mer, allows customizable fragmentation patterns. DP end presents novel efficient strategy enhancing MS/MS amplifying capacity polymers.

Language: Английский

Citations

Complex Sequence‐Defined Heteropolymers Enable Controlled Film Growth in Layer‐By‐Layer Assembly DOI

Ranajit Barman,

Michel Tschopp,

Laurence Charles

et al.

Macromolecular Rapid Communications, Journal Year: 2024, Volume and Issue: 45(22)

Published: Aug. 6, 2024

Abstract Digitally‐encoded poly(phosphodiesters) ( d ‐PPDE) with highly complex primary structures are evaluated for layer‐by‐layer (LbL) assembly. To be easily decoded by mass spectrometry (MS), these digital polymers contain many different monomers: 2 coding units allowing binary encryption, 1 cleavable spacer controlled MS fragmentation, and 3 tags fragment identification. These heteropolymers therefore composed of 6 motifs. Despite this strong sequence heterogeneity, it is found that they enable a LbL film formation. For instance, regular growth observed when alternating the deposition negatively‐charged ‐PPDE positively‐charged poly(allyl amine hydrochloride) (PAH). Yet, in approach, interdistance between consecutive coded layers remains relatively small, which may an issue data storage applications, especially selective decoding stored information. Using poly(sodium 4‐styrene sulfonate) (PSS) as intermediate non‐coded polyanion, shown can achieved, while still maintaining growth. Last but not least, work small molecular weight (i.e., significantly smaller than those PAH PSS) enables

Language: Английский

Citations

Scalable design of uniform oligourethanes for impact study of chain length, sequence and end groups on thermal properties DOI

Jens Van Hoorde, Nezha Badi, Filip Du Prez

et al.

Polymer Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

A bidirectional growth-based protocol is used for the synthesis of a series sequence-defined oligourethanes in large scale (50 g). The effect chain length, sequence and end group functionalities on thermal properties evaluated.

Language: Английский

Citations