Conversion Therapy Strategy: A Novel GPC3-Targeted Multimodal Organic Phototheranostics Platform for Mid-late-stage Hepatocellular Carcinoma DOI Creative Commons
Fan Wu, Xin Kuang,

Shaocong Deng

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 25, 2024

Abstract Hepatocellular carcinoma (HCC) is typically diagnosed at an intermediate to advanced stage, making surgical treatment unfeasible. Conversion therapy aims reduce tumor improve hepatic resection feasibility, and lower recurrence rates. Since traditional therapies are often accompanied by uncertainty of efficacy, there urgent need explore new strategies. Near-infrared phototheranostics technology provides a way for HCC diagnosis its excellent optical properties. However, complex preparation poor biocompatibility probes limit clinical application. In this study, we developed fluorescence/magnetic resonance dual-modality imaging (FLI/MRI) as well photothermal/photodynamic (PTT/PDT) GPC3-targeted multifunctional probe, IR820-GPC3-Gd NPs (IGD NPs), the efficiency conversion HCC. The IGD were simply prepared with IR820 core. It made connecting HCC-specific targeting molecule GPC3 peptide MRI agent DOTA-Gd through click chemistry. target cells GPC3, releasing heat reactive oxygen species (ROS) under noninvasive 808 nm laser irradiation size achieve downstaging. High-sensitivity FLI/MRI precisely delineates boundaries, providing real-time navigation prognosis assessment. This novel probe offers feasible effective option

Language: Английский

Pretargeted Multimodal Tumor Imaging by Enzymatic Self-Immobilization Labeling and Bioorthogonal Reaction DOI

Yinxing Miao,

Yuqi Wang, Yefeng Chen

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 12, 2025

Covalent modification of cell membranes has shown promise for tumor imaging and therapy. However, existing membrane labeling techniques face challenges such as slow kinetics poor selectivity cancer cells, leading to off-target effects suboptimal in vivo efficacy. Here, we present an enzyme-triggered self-immobilization strategy, termed E-SIM, which enables rapid selective with bioorthogonal trans-cycloctene (TCO) handles vivo. E-SIM utilizes P-TCO, alkaline phosphatase (ALP) responsive quinone methide (QM) precursor a TCO group, facilitating the conjugation high-density onto via proximity labeling. These groups then react efficiently tetrazine (Tz)-bearing reporters fast reaction, resulting significant enrichment various sizes modalities on membranes. We demonstrate efficacy reaction pretargeted multimodality tumors Notably, achieve efficient installation Tz-modified Renilla luciferase cells vivo, thereby offering highly sensitive bioluminescence signals detecting guiding surgical removal small human HepG2 liver peritoneal metastases. represents robust tool precise complex environments, feasible multimodal applications.

Language: Английский

Citations

1
Insights into Molecular Lanthanide Complexes: Construction, Properties and Bioimaging and Biosensing Applications DOI
Yanling Yang, Xiaoming Hu, Zhèn Yáng

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 12, 2024

Abstract The lanthanide complex, characterized by narrow emission bands, large Stokes shifts, long luminescence lifetime, robust resistance to photobleaching, and magnetic properties, affords significant promise for molecular imaging biosensing applications. While most complexes emitting visible light are extensively studied cellular‐level detection bioimaging, near‐infrared‐emitted (700–1700 nm) compounds exhibit more favorable properties bioimaging biosensing, ascribed from deeper penetration reduced bleaching effects. Besides, the complex with higher resonance signals can enhance image resolution sensitivity, offering precise analysis. However, widespread use of high‐efficiency is currently impeded lack design approaches improving sensitization chromophores extended excitation wavelengths. In this review, some fundamental theories strategies summarized imaging, lifetime‐engineered dual‐modal now highlight a selection review addresses existing gap in literature proposing feasible fluorescent properties. Concurrently, future challenges opportunities field probes discussed.

Language: Английский

Citations

7
Tandem Targeting and Dual Aggregation of an AIEgen for Enhanced Near-Infrared Fluorescence Imaging of Tumors DOI
Yu Deng, Lingling Xu, Xiaoyang Liu

et al.

Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: 146(37), P. 25462 - 25466

Published: Sept. 6, 2024

Near-infrared (NIR) aggregation-induced emission luminogens (AIEgens) are excellent probes for tumor imaging, but there still is space to improve their imaging specificity and sensitivity. In this work, a strategy of tandem targeting dual aggregation an AIEgen proposed achieve these two purposes. An AIEgen, β-tBu-Ala-Cys(StBu)-Lys(Biotin)-Pra(QMT)-CBT (

Language: Английский

Citations

5
Stimulus-activated ribonuclease targeting chimeras for tumor microenvironment activated cancer therapy DOI Creative Commons
Yuqi Zhang,

Jinfeng Zhu,

Ling Qiu

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 3, 2025

RNA degradation using ribonuclease targeting chimeras (RiboTACs) is a promising approach for cancer therapy. However, potential off-target serious issue. Here, RiboTAC designed tumor microenvironment triggered activation. The activated (TaRiboTAC) incorporates two pre-miR-21 binders, near-infrared fluorophore IR780, an RGD peptide and phenylboronic acid caged recruiter. recruiter embedded in the molecule exposed acidic pH, cage removed by H2O2 making TaRiboTAC responsive to high levels microenvironment. It shown targets tissue degrades pre-miR-21. of TaRiboTACs significantly increases radiotherapeutic susceptibility cells achieving efficient suppression human lung adenocarcinoma A549 tumors living mice.

Language: Английский

Citations

0
Architecture design of improved near-infrared hemicyanine dyes for biosensing and bioimaging DOI

Guohui Deng,

Gangwei Jiang, Lin Yuan

et al.

Chinese Science Bulletin (Chinese Version), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Biotin Receptor-Targeting PtIV Oxygen Carrying Prodrug Amphiphile for Alleviating Tumor Hypoxia Induced Immune Chemotherapy Suppression DOI

Kaichuang Sun,

Xiaodan Wei, Shangcong Han

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Platinum (Pt)-based chemotherapeutic agents, known for their potent cytotoxicity, are extensively used in clinical oncology. However, therapeutic efficacy is severely limited by a variety of factors, particularly the hypoxic tumor microenvironment (TME), which not only impedes effective drug delivery but also triggers immune suppression, further diminishing antitumor effects Pt drugs. In response to these challenges, we have developed biotin receptor (BR)-targeting oxaliplatin (OXA)-based PtIV prodrug, named Lipo-OPtIV-BT, could encapsulate hemoglobin (Hb) as an oxygen carrier, forming PtIV-loaded lipid nanoparticles (Hb@BTOPtIV). The design Hb@BTOPtIV aims address dual issues poor and suppression effectively increasing local tension TME. Notably, our findings demonstrate that cytotoxic BR-targeting prodrug increased levels synergistically reverse microenvironment, leading improved efficacy. We observed significantly biodistribution drug, enabling it preferentially accumulate regions. Importantly, enhanced oxygenation within TME plays critical role reshaping landscape tumor, promoting more favorable environment chemotherapy. This reversal evidenced infiltration T cells reduced regulatory (Tregs) tumor. These highlight promising potential using amphiphiles improve accumulation at sites counteract immunosuppression induced hypoxia.

Language: Английский

Citations

0
In situ self-assembled peptide nanoparticles improves anti-hepatic fibrosis effect DOI

Wenbin Liu,

Beibei Wang,

Mengxing Wei

et al.

Journal of Materials Chemistry B, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Antagonistic peptide Leu-Ser-Lys-Leu (LSKL) is capable of blocking the transforming growth factor-β1 (TGF-β1) signaling pathway and exhibits anti-fibrotic effects. Herein, we constructed LSKL nanoparticles (NPs) in situ based on an alkaline phosphatase (ALP)-instructed self-assembly strategy for improving its specific therapeutic effect against liver fibrosis.

Language: Английский

Citations

0
Self‐Assembly of Noncanonical Peptides: A New Frontier in Cancer Therapeutics and Beyond DOI Creative Commons
Lin Bai, Meihui Yi, Bing Xu

et al.

Macromolecular Bioscience, Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

Abstract In addition to the 20 standard amino acids that form building blocks of proteins, nature employs alternative create specialized “noncanonical peptides.” These unique peptides, found in organisms from bacteria humans, often exhibit unconventional structures and functionalities, playing critical roles modulating cellular processes, particularly as antibiotics. Their potential has attracted significant interest for designing novel functional materials based on noncanonical peptides. This review highlights recent advances generation application peptide assemblies. It begins with a definition including classic examples showcase their distinct useful biological activities. Then applications assemblies developing anticancer therapeutics are discussed, focusing representative studies demonstrate efficacy versatility targeting tumor cells. Beyond oncology, it is explored how have been utilized biomaterials, regenerative medicine, molecular imaging catalysis. Finally, perspectives offered future directions this rapidly evolving field, emphasizing exciting opportunities remaining challenges will drive continued innovation applying peptide‐based

Language: Английский

Citations

0
Tandem Reaction-Powered Near-Infrared Fluorescent Molecular Reporter for Real-Time Imaging of Lung Diseases DOI Creative Commons
Yan Hu, Hongshuai Zhang,

Yiteng Ding

et al.

Chemical Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Diabetes and its complications have drawn growing research attention due to their detrimental effects on human health. Although optical probes been used help understand many aspects of diabetes, the lung diseases caused by diabetes remain unclear rarely explored. Herein, a tandem-reaction (TR) strategy is proposed based adjacent diol esterification-crosslinking reaction nicotinamide reduction adenine dinucleotide (NADH) design lung-targeting near-infrared (NIR) small molecule probe (NBON) for accurate imaging diabetic diseases. NBON was designed coupling phenylboronic acid analog that can form borate ester bonds reversibly binding with NADH via an reaction. Streptozotocin (STZ)-induced mice metformin (MET)/epalrestat (EPS)-repaired model studies demonstrated allowed sensitive disease diagnosis therapeutic monitoring. The antioxidant mechanism which EPS alleviates studied first time in living cells vivo. Furthermore, successfully applied detection tumors metastases. Overall, this work provides general platform NIR design, advances development mechanistic

Language: Английский

Citations

0
Conversion Therapy Strategy: A Novel GPC3-Targeted Multimodal Organic Phototheranostics Platform for Mid-late-stage Hepatocellular Carcinoma DOI Creative Commons
Fan Wu, Xin Kuang,

Shaocong Deng

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: 30, P. 101442 - 101442

Published: Jan. 2, 2025

Hepatocellular carcinoma (HCC) is typically diagnosed at intermediate to advanced stage, making surgical treatment unfeasible. Conversion therapy aims reduce tumor improve hepatic resection feasibility, and lower recurrence rates. Since traditional therapies are often accompanied by uncertainty of efficacy, there an urgent need explore new strategies. Near-infrared phototheranostics technology provides a way for HCC diagnosis its excellent optical properties. However, complex preparation poor biocompatibility probes limit clinical application. In this study, we developed fluorescence/magnetic resonance dual-modality imaging (FLI/MRI) as well photothermal/photodynamic (PTT/PDT) GPC3-targeted multifunctional probe, IR820-GPC3-Gd NPs (IGD NPs), the efficiency conversion HCC. The IGD were simply prepared with IR820 core. Conjugating HCC-specific targeting molecule GPC3 peptide MRI agent DOTA-Gd through click chemistry. target cells GPC3, releasing heat reactive oxygen species (ROS) under noninvasive 808 nm laser irradiation size achieve downstaging. High-sensitivity FLI/MRI precisely delineates boundaries, providing real-time navigation prognosis assessment. This novel probe offers feasible effective option

Language: Английский

Citations

0