MAS NMR experiments of corynebacterial cell walls: Complementary 1H- and CPMAS CryoProbe-enhanced 13C-detected experiments

Journal of Magnetic Resonance, Journal Year: 2024, Volume and Issue: 364, P. 107708 - 107708

Published: June 6, 2024

Bacterial cell walls are gigadalton-large cross-linked polymers with a wide range of motional amplitudes, including rather rigid as well highly flexible parts. Magic-angle spinning NMR is powerful method to obtain atomic-level information about intact walls. Here we investigate sensitivity and content different homonuclear

Language: Английский

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The biofilm matrix protects Bacillus subtilis against hydrogen peroxide

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 12, 2025

Abstract Biofilms formed by Bacillus subtilis confer protection against environmental stressors through extracellular polysaccharides (EPS) and sporulation. This study investigates the roles of these biofilm components in resistance to hydrogen peroxide, a common reactive oxygen species source disinfectant. Using wild-type mutant strains deficient EPS or sporulation, colonies were cultivated at various maturation stages exposed peroxide. EPS-deficient biofilms exhibited reduced resilience, particularly early stages, highlighting structural protective importance matrix. Mature demonstrated additional mechanisms, potentially involving TasA protein fibers. In contrast, sporulation showed limited contribution peroxide resistance, as survival was primarily matrix-dependent. These findings underscore necessity targeting other matrix anti-biofilm strategies, suggesting that peroxide-based disinfection could be enhanced combining it with complementary sporicidal treatments. advances our understanding contributing development more effective sterilization protocols.

Language: Английский

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Time-resolved compositional and dynamics analysis of biofilm maturation and dispersal via solid-state NMR spectroscopy

npj Biofilms and Microbiomes, Journal Year: 2025, Volume and Issue: 11(1)

Published: Jan. 29, 2025

Dispersal plays a crucial role in the development and ecology of biofilms. While extensive studies focused on elucidating molecular mechanisms governing this process, few have characterized associated temporal changes composition structure. Here, we employed solid-state nuclear magnetic resonance (NMR) techniques to achieve time-resolved characterization Bacillus subtilis biofilms over 5-day period. The mature biofilm, established within 48 h, undergoes significant degradation following 72 h. steepest decline proteins precedes that exopolysaccharides, likely reflecting their distinct spatial distribution. Exopolysaccharide sugar units display clustered patterns, suggesting presence polysaccharide types. A sharp rise aliphatic carbon signals day 4 probably corresponds surge biosurfactant production. Different dynamic regimes respond differently dispersal: mobile domain exhibits increased rigidity, while rigid remains stable. These findings provide novel insights perspectives complex process biofilm dispersal.

Language: Английский

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The biofilm matrix protects Bacillus subtilis against hydrogen peroxide

Biofilm, Journal Year: 2025, Volume and Issue: unknown, P. 100274 - 100274

Published: March 1, 2025

Biofilms formed by Bacillus subtilis confer protection against environmental stressors through extracellular polysaccharides (EPS) and sporulation. This study investigates the roles of these biofilm components in resistance to hydrogen peroxide, a common reactive oxygen species source disinfectant. Using wild-type mutant strains deficient EPS or sporulation, colonies were cultivated at various maturation stages exposed peroxide. EPS-deficient biofilms exhibited reduced resilience, particularly early stages, highlighting structural protective importance matrix. Mature demonstrated additional mechanisms, potentially involving TasA protein fibers and/or surface layer (BslA). In contrast, sporulation showed limited contribution peroxide resistance, as survival was primarily matrix-dependent. These findings underscore necessity targeting other matrix anti-biofilm strategies, suggesting that peroxide-based disinfection could be enhanced combining it with complementary sporicidal treatments. advances our understanding contributing development more effective sterilization protocols.

Language: Английский

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Solid-state NMR Observation of Chitin in Whole Cells by Indirect 15N Detection with NC, NCC, CNC and CNCC polarization transfers

Solid State Nuclear Magnetic Resonance, Journal Year: 2025, Volume and Issue: unknown, P. 102002 - 102002

Published: March 1, 2025

Language: Английский

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Proton-Detected Solid-State NMR for Deciphering Structural Polymorphism and Dynamic Heterogeneity of Cellular Carbohydrates in Pathogenic Fungi

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Carbohydrate polymers in their cellular context display highly polymorphic structures and dynamics essential to diverse functions, yet they are challenging analyze biochemically. Proton-detection solid-state NMR spectroscopy offers high isotopic abundance sensitivity, enabling rapid high-resolution structural characterization of biomolecules. Here, an array 2D/3D 1 H-detection techniques tailored investigate polysaccharides fully protonated or partially deuterated cells three prevalent pathogenic fungi: Rhizopus delemar , Aspergillus fumigatus Candida albicans representing filamentous species yeast forms. Selective detection acetylated carbohydrates reveals fifteen forms N-acetylglucosamine units R. chitin, which coexists with chitosan as separate domains associates proteins only at limited sites. This is supported by distinct order parameters effective correlation times motions, analyzed through relaxation measurements model-free analysis. Five α-1,3-glucan origins were identified A. important for this buffering polysaccharide perform roles supporting wall mechanics regenerating soft matrix under antifungal stress. Eight α-1,2-mannan sidechain variants C. resolved, highlighting the crucial role mannan sidechains maintaining interactions other cell preserve integrity. These methodologies provide novel insights into functional key fungal create new opportunities exploring carbohydrate biosynthesis modifications across organisms.

Language: Английский

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The evolving role of solid state nuclear magnetic resonance methods in studies of amyloid fibrils

Current Opinion in Structural Biology, Journal Year: 2025, Volume and Issue: 92, P. 103043 - 103043

Published: April 8, 2025

Language: Английский

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Mass transfer in heterogeneous biofilms: Key issues in biofilm reactors and AI-driven performance prediction

Environmental Science and Ecotechnology, Journal Year: 2024, Volume and Issue: 22, P. 100480 - 100480

Published: Aug. 29, 2024

Biofilm reactors, known for utilizing biofilm formation cell immobilization, offer enhanced biomass concentration and operational stability over traditional planktonic systems. However, the dense nature of biofilms poses challenges substrate accessibility to cells efficient release products, making mass transfer efficiency a critical issue in these Recent advancements have unveiled intricate, heterogeneous architecture biofilms, contradicting earlier view them as uniform, porous structures with consistent properties. In this review, we explore six reactor configurations their potential combinations, emphasizing how spatial arrangement within reactors influences overall performance. Furthermore, discuss apply artificial intelligence processing measurement data predicting This review highlights role environmental energy sectors, paving way future innovations biofilm-based technologies broader applications.

Language: Английский

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Cyanobacterial biofilms: from natural systems to applications

Trends in biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 1, 2024

Cyanobacteria are the ancestors of oxygenic photosynthesis. Fueled by light and water, their ability to reduce CO

Language: Английский

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