ChemBioChem,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 10, 2024
The
integration
of
nanomaterials
with
DNA-based
systems
has
emerged
as
a
transformative
approach
in
biosensing
and
therapeutic
applications.
Unique
features
DNA,
like
its
programmability
specificity,
complement
the
diverse
functions
nanomaterials,
leading
to
creation
advanced
for
detecting
biomarkers
delivering
treatments.
Here,
we
review
developments
DNA-nanomaterial
conjugates,
emphasizing
their
enhanced
functionalities
potential
across
various
biomedical
We
first
discuss
methodologies
synthesizing
these
distinguishing
between
covalent
non-covalent
interactions.
then
categorize
DNA-nanomaterials
conjugates
based
on
properties
DNA
involved,
respectively.
probes
are
classified
by
application
into
or
uses,
and,
several
highlighted
recent
progress
living
biological.
Finally,
current
challenges
future
prospects
this
field,
anticipating
that
significant
will
greatly
enhance
precision
medicine.
ACS Nano,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 21, 2025
Nucleic
acid
therapeutics
represent
a
highly
promising
treatment
approach
in
modern
medicine,
treating
diseases
at
the
genetic
level.
However,
these
face
numerous
challenges
practical
applications,
particularly
regarding
their
stability,
effectiveness,
cellular
uptake
efficiency,
and
limitations
delivering
them
specifically
to
target
tissues.
To
overcome
obstacles,
researchers
have
developed
various
innovative
delivery
systems,
including
viral
vectors,
lipid
nanoparticles,
polymer
inorganic
protein
carriers,
exosomes,
antibody
oligonucleotide
conjugates,
DNA
nanostructure-based
systems.
These
systems
enhance
therapeutic
efficacy
of
nucleic
drugs
by
improving
targeting
specificity,
half-life
vivo.
In
this
review,
we
systematically
discuss
different
types
drugs,
analyze
major
barriers
encountered
delivery,
summarize
current
research
progress
emerging
We
also
highlight
latest
advancements
application
for
diseases,
infectious
cancer,
brain
wound
healing.
This
review
aims
provide
comprehensive
overview
drug
systems'
status
future
directions
integrating
nanotechnology,
biomaterials
science,
gene
editing
technologies,
emphasizing
transformative
potential
precision
medicine.
Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 13, 2025
An
entropy-driven
catalysis
(EDC)
strategy
is
appealing
for
amplified
bioimaging
of
microRNAs
in
living
cells;
yet,
complex
operation
procedures,
lacking
cell
selectivity,
and
insufficient
accuracy
hamper
its
further
applications.
Here,
we
introduce
an
ingenious
all-in-one
DNA
nanomachine
(termed
as
AIO-EDN),
which
can
be
triggered
by
endogenous
apurinic/apyrimidinic
endonuclease
1
(APE1)
to
achieve
tumor
cell-selective
dual-mode
imaging
microRNA.
Compared
with
the
traditional
EDC
strategy,
integrated
design
AIO-EDN
achieves
autocatalytic
signal
amplification
without
extra
fuel
strands.
Moreover,
leverages
APE1
overexpressed
cancer
cells
activate
reaction,
which,
however,
exerts
no
target
sensing
activity
normal
cells.
Combining
fluorescence-
surface-enhanced
Raman
scattering
(FL/SERS)
techniques,
this
exhibits
significantly
improved
selectivity
microRNA
This
study
provides
a
new
paradigm
develop
EDC-based
platform
shows
great
potential
in-depth
diagnosis
high
precision.
Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 17, 2025
A
sensitive
fluorescence
biosensor
was
developed
for
microcystin-LR
(MC-LR)
detection
using
H1,
H2,
and
H3
DNA
probes
as
sensing
elements.
The
aptamer
in
H1
can
recognize
the
target.
H2
labeled
with
FAM
BHQ.
MC-LR
binding
will
activate
self-assemblies
through
toehold-mediated
strand
displacement.
In
formed
products
(MC-LR/H1/nH2/nH3),
BHQ
be
separated
a
high
signal
observed
assay.
is
limit
of
53
fM.
We
further
constructed
several
logic
circuits
(AND-AND
cascaded
circuit,
feedforward
resource
allocation
circuit)
MC-LR,
MC-LA,
MC-YR
three
inputs.
numbers
0
1
are
used
to
code
input
output
signals.
AND-AND
cascade
circuit
produce
only
(111)
combination.
MC-LA
signals,
inhibit
self-assembly
execute
negative
operation.
Through
rational
design
probe
hybridizations
on
four
different
magnetic
beads
(MBs),
achieve
an
intelligent
information.
Our
proposed
not
provide
platform
microcystin
but
also
serve
smart
system
sensing.
Advanced Functional Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 23, 2025
Abstract
Synthetic
catalytic
DNAzyme
circuits
have
gained
great
attention
as
versatile
theranostic
toolboxes,
but
their
accuracy
and
efficiency
is
restricted
by
uncontrolled
signal
leakage
inefficient
circuitry
activation.
Here,
a
trinity
circuit
(TriDC)
constructed
using
unique
logic
gate
bioswitchable
DNA
tetrahedral
walker@MnO
2
nanosheets
structure.
The
AND
walker,
composed
of
dual
allosteric
(termed
“commander”
“soldier”)
scaffold,
used
to
execute
imaging‐guided
gene
therapy
avoiding
undesired
cascade
“walking”
in
confined
space.
Glutathione‐mediated
reduction
MnO
provides
secondary
confinement
stack
the
walker
situ,
offering
abundant
Mn
2+
cofactor
achieve
self‐sufficiency
induce
chemo‐dynamic
therapy.
structure
increases
local
reaction
concentrations
≈100‐fold
according
collision
frequency
model,
enabling
detection
microRNA‐10b
microRNA‐155
at
limit
98.97
p
m
,
thereby
facilitating
precise
imaging
breast
cancer
cells.
combined
gene/chemo‐dynamic
achieves
66.2%
tumor
growth
inhibition
efficiency.
TriDC
offers
novel
insights
into
theranostics
also
presents
paradigm
for
situ
implementation
higher‐order
circuits.
Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 16, 2025
Precise
and
highly
sensitive
identification
of
cancer
cells
plays
a
pivotal
role
in
early
detection,
diagnosis,
effective
treatment.
While
DNA
logic
circuits
have
shown
great
promise
as
diagnostic
tools,
their
practical
application
has
been
hindered
by
inadequate
sensitivity
arising
from
limited
signal
amplification
capabilities
complex
biological
matrices.
To
address
this
issue,
we
constructed
localized
circuit
(LDC)
equipped
with
cascaded
amplifiers
introducing
Y-shaped
AND-gate
module
three
hairpin
amplifier
modules
into
tetrahedron.
The
gate
is
activated
only
the
simultaneous
presence
two
cancer-specific
biomarkers:
intracellular
microRNA-21
(miR-21)
flap
endonuclease
1
(FEN1).
Upon
activation,
releases
output
strands
that
trigger
assembly
amplifiers,
initiating
strand
displacement
cascade
generates
significantly
enhanced
fluorescent
signal.
LDC
exhibits
remarkable
detection
limits
82.5
pM
for
miR-21
0.015
U/mL
FEN1.
Fluorescence
assays
demonstrate
achieves
15.5-fold
improvement
over
without
5.2-fold
compared
to
nonlocalized
circuits.
enables
dual
biomarkers,
generating
amplified
signals
exclusively
tumor
expressing
both
FEN1,
thus
allowing
precise
discrimination
between
cancerous
healthy
cells.
Furthermore,
demonstrated
system
vivo
imaging,
effectively
differentiating
normal
tissues.
This
work
highlights
potential
proposed
cascade-amplification
strategy
tumor-specific
paving
way
diagnosis
Advanced Science,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 21, 2025
Photodynamic
therapy
(PDT)
is
a
promising
strategy
for
the
treatment
of
skin-related
tumors
including
cutaneous
squamous
cells
carcinoma
(cSCC).
However,
it
hard
to
balance
dosage
off-target
phototoxicity
while
maintaining
satisfactory
therapeutic
effect.
In
addition,
oxygen-dependent
photosensitizers
(PSs)
are
affected
by
tumor
hypoxic
environment,
which
further
causes
inefficient
photocatalysis
and
reduces
Herein,
an
intelligent
DNA
nanomachine
based
on
tetrahedral
framework
proposed,
incorporated
with
tumor-targeted
aptamer
specific
miRNA-responsive
hairpin
catalytic
assembly
(HCA),
precise
high-efficient
cSCC.
After
aptamer-mediated
targeted
delivery,
cSCC-specific
miRNA
selected
tissue
sequencing
analysis
used
activateHCA,
amplifying
PSs
controllably
releasing
chemotherapeutic
drugs.
Sequential
recognition
can
greatly
improve
tumor-specific
accumulation
high-dose
activation.
Moreover,
hemin
into
catalytically
produce
oxygen.
vitro
in
vivo
experiments
demonstrated
that
this
improved
anti-tumor
effect
realized
effective
ablation
cSCC
mice,
barely
systemic
toxicity
inflammation.
It
anticipated
will
promote
biomedical
applications
provide
option
non-invasive
skin-associated
tumors.
Small,
Journal Year:
2025,
Volume and Issue:
unknown
Published: May 12, 2025
Abstract
Inspired
by
the
advantages
of
biological
cascade
catalytic
systems,
it
has
been
devoted
to
discovery
novel
oxidase‐based
systems
for
disease
monitoring.
However,
low
stability,
easy
inactivation,
and
poor
reproducibility
oxidase
significantly
limit
their
practical
applications.
Immobilization
can
be
enabled
protect
them
from
external
mediators
improve
efficiency
reproducibility.
Notably,
substrate
channels
spatial
confinement
play
an
essential
role
in
construction
immobilized
nanoreactors
enhance
overall
activity.
Moreover,
nanozymes,
a
class
enzyme
mimics,
have
not
only
enzyme−like
activity
but
also
high
stability
tunable
properties,
which
bolster
development
nanoreactors.
Herein,
recent
advances
assembly
reactors
involving
enzymes/nanozymes
are
described.
The
importance
channeling
distribution
regulating
nanoreactor
is
highlighted.
Then,
along
with
in−depth
discussion
biosensors
monitoring,
design
application
innovative
devices
based
on
these
sensing
principles
summarized,
including
microfluidic
hydrogel−based
platforms,
test
paper
technologies.
Finally,
challenges
prospects
briefly
discussed
prospected.
